Journal of Molecular Biology

Journal: Journal of Molecular Biology

Abbreviation

J. Mol. Biol.

Publisher

Elsevier

ISSN

0022-2836
1089-8638

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Publications1 - 10 of 183

Crystallization and preliminary X-ray analysis of two different forms of mitochondrial creatine kinase from chicken cardiac muscle
Schnyder, Thomas; Sargent, David F.; Richmond, Timothy J.; et al. (1990)
Journal of Molecular Biology
HURP wraps microtubule ends with an additional tubulin sheet that has a novel conformation of tubulin
Santarella, Rachel A.; Koffa, Maria D.; Tittmann, Peter; et al. (2007)
Journal of Molecular Biology
Salt Anions Promote the Conversion of HypF-N into Amyloid-Like Oligomers and Modulate the Structure of the Oligomers and the Monomeric Precursor State
Campioni, Silvia; Mannini, Benedetta; López-Alonso, Jorge P.; et al. (2012)
Journal of Molecular Biology
Quantitative Selection Analysis of Bacteriophage φcbK Susceptibility in Caulobacter crescentus
Christen, Matthias; Beusch, Christian; Bösch, Yvonne; et al. (2016)
Journal of Molecular Biology
Classical molecular genetics uses stringent selective conditions to identify mutants with distinct phenotypic responses. Mutations giving rise to less pronounced phenotypes are often missed. However, to gain systems-level insights into complex genetic interaction networks requires genome-wide assignment of quantitative phenotypic traits. In this paper, we present a quantitative selection approach coupled with transposon sequencing (QS-TnSeq) to globally identify the cellular components that orchestrate susceptibility of the cell cycle model bacterium Caulobacter crescentus toward bacteriophage φCbK infection. We found that 135 genes representing 3.30% of the Caulobacter genome exhibit significant accumulation of transposon insertions upon φCbK selection. More than 85% thereof consist of new factors not previously associated with phage φCbK susceptibility. Using hierarchical clustering of dose-dependent TnSeq datasets, we grouped these genes into functional modules that correlate with different stages of the φCbK infection process. We assign φCbK susceptibility to eight new genes that represent novel components of the pilus secretion machinery. Further, we demonstrate that, from 86 motility genes, only seven genes encoding structural and regulatory components of the flagellar hook increase phage resistance when disrupted by transposons, suggesting a link between flagellar hook assembly and pili biogenesis. In addition, we observe high recovery of Tn5 insertions within regulatory sequences of the genes encoding the essential NADH:ubiquinone oxidoreductase complex indicating that intact proton motive force is crucial for effective phage propagation. In sum, QS-TnSeq is broadly applicable to perform quantitative and genome-wide systems-genetics analysis of complex phenotypic traits.
Random circular permutation of DsbA reveals segments that are essential for protein folding and stability
Hennecke, Jens; Sebbel, Peter; Glockshuber, Rudi (1999)
Journal of Molecular Biology
Prion Protein mPrP F1751A (121-231)
Christen, Barbara; Hornemann, Simone; Damberger, Fred; et al. (2012)
Journal of Molecular Biology
An Intrinsic Degradation Tag on the ClpA C-Terminus Regulates the Balance of ClpAP Complexes with Different Substrate Specificity
Maglica, Zeljka; Striebel, Frank; Weber-Ban, Eilika (2008)
Journal of Molecular Biology
Cardiolipin clusters and membrane domain formation induced by mitochondrial proteins
Epand, Raquel F.; Tokarska-Schlattner, Malgorzata; Schlattner, Uwe; et al. (2007)
Journal of Molecular Biology
Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions
de Picciotto, Seymor; Dickson, Paige M.; Traxlmayr, Michael W.; et al. (2016)
Journal of Molecular Biology
Dlx5 Homeodomain:DNA Complex: Structure, Binding and Effect of Mutations Related to Split Hand and Foot Malformation Syndrome
Proudfoot, Andrew; Axelrod, Herbert L.; Geralt, Michael; et al. (2016)
Journal of Molecular Biology
The Dlx5 homeodomain is a transcription factor related to the Drosophila distal-less gene that is associated with breast and lung cancer, lymphoma, Rett syndrome and osteoporosis in humans. Mutations in the DLX5 gene have been linked to deficiencies in craniofacial and limb development in higher eukaryotes, including split hand and foot malformation 1 in humans. Our characterization of a Dlx5 homeodomain:(CGACTAATTAGTCG)2 complex by NMR spectroscopy paved the way for determination of its crystal structure at 1.85 Å resolution that enabled rationalization of the effects of disease-related mutations on the protein function. A Q186H mutation linked to split hand and foot malformation 1 likely affects affinity of DNA binding by disrupting water-mediated interactions with the DNA major groove. A more subtle effect is implicated for the Q178P mutation, which is not in direct contact with the DNA. Our data indicate that these mutations diminish the ability of the Dlx5 homeodomain to recognize and bind target DNAs, and they likely destabilize the formation of functional complexes.

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Journal: Journal of Molecular Biology

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