Journal of Materials Chemistry B

Journal: Journal of Materials Chemistry B

Abbreviation

J. Mater. Chem. B

Publisher

Royal Society of Chemistry

ISSN

2050-7518
2050-750X

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Publications 1 - 10 of 23

Micelle vs. vesicle formation controlled by distal functionalization of C-60-PEG conjugates
Tiu, Elisha G.V.; Liosi, Korinne; Aroua, Safwan; et al. (2017)
Journal of Materials Chemistry B
Noncytotoxic artificial bacterial flagella fabricated from biocompatible ORMOCOMP and iron coating
Qiu, Famin; Zhang, Li; Peyer, Kathrin E.; et al. (2014)
Journal of Materials Chemistry B
DNA protection against ultraviolet irradiation by encapsulation in a multilayered SiO2/TiO2 assembly
Paunescu, Daniela; Mora, Carlos A.; Puddu, Michela; et al. (2014)
Journal of Materials Chemistry B
Selective Sensing of Isoprene by Ti-doped ZnO for Breath Diagnostics
Güntner, Andreas; Pineau, Nicolay J.; Chie, Donovan; et al. (2016)
Journal of Materials Chemistry B
Characterization of carbon-coated magnetic nanoparticles using clinical blood coagulation assays
Bircher, Lukas; Theusinger, Oliver M.; Locher, Silvan; et al. (2014)
Journal of Materials Chemistry B
Intravascular application of magnetic nanocarriers is a critical step in the development of new therapeutic strategies, including magnetic drug targeting or hyperthermia. However, injection of particulate matter bears the intrinsic risk of contact activation of the blood coagulation cascade. In this work, we use point-of-care assays to study coagulation dynamics and clotting parameters in blood samples exposed to relevant concentrations of surface-functionalized carbon-coated iron carbide nanomagnets using unmodified nanomagnets and poly(ethylene)glycol-functionalized nanomagnets with different end-groups, including –OCH3, –NH2, –COOH, –IgG, and –ProteinA-protected-IgG (–IgG-ProtA). Silica nanoparticles with a comparable surface area are used as a reference material. For magnetic nanoparticles, we observe a decrease in clotting time by 25% compared to native blood at concentrations of 1 mg mL−1, independent of the surface functionalization, and only minor differences in receptor expression on platelets (GP-IIb-IIIa, CD62, and CD63) relative to control samples were observed. Interestingly, the inter-subject variance of the clotting time is similar to the nanoparticle-induced effect in a single subject with average clotting time. Whilst the present study is based on in vitro assays and a small group of healthy blood donors, the comparison to broadly used silica nanoparticles, and the fact that experimental intergroup variability is comparable to the observed effects from the carbon-coated nanomagnets suggests continuing investigations on their potential clinical use.
Enzyme-responsive nanoparticles: enhancing the ability of endolysins to eradicate Staphylococcus aureus biofilm
Blanco Massani, Mariana; To, Dennis; Meile, Susanne; et al. (2024)
Journal of Materials Chemistry B
Stimuli-responsive nanomaterials show promise in eradicating Staphylococcus aureus biofilm from implants. Peptidoglycan hydrolases (PGHs) are cationic antimicrobials that can be bioengineered to improve the targeting of persisters and drug-resistant bacteria. However, these molecules can be degraded before reaching the target and/or present limited efficacy against biofilm. Therefore, there is an urgent need to improve their potency. Herein, PGH-polyphosphate nanoparticles (PGH-PP NPs) are formed by ionotropic gelation between cationic PGHs and anionic polyphosphate, with the aim of protecting PHGs and delivering them at the target site triggered by alkaline phosphatase (AP) from S. aureus biofilm. Optimized conditions for obtaining M23-PP NPs and GH15-PP NPs are presented. Size, zeta potential, and transmission electron microscopy imaging confirm the nanoscale size. The system demonstrates outstanding performance, as evidenced by a dramatic reduction in PGHs' minimum inhibitory concentration and minimum bactericidal concentration, together with protection against proteolytic effects, storage stability, and cytotoxicity towards the Caco-2 and HeLa cell lines. Time-kill experiments show the great potential of these negatively charged delivery systems in overcoming the staphylococcal biofilm barrier. Efficacy under conditions inhibiting AP proves the enzyme-triggered delivery of PGHs. The enzyme-responsive PGH-PP NPs significantly enhance the effectiveness of PGHs against bacteria residing in biofilm, offering a promising strategy for eradicating S. aureus biofilm.
Covalently functionalized amide cross-linked hydrogels from primary amines and polyethylene glycol acyltrifluoroborates (PEG-KATs)
Schauenburg, Dominik; Galvez, Alberto O.; Bode, Jeffrey W. (2018)
Journal of Materials Chemistry B
Targeting transdifferentiated hepatic stellate cells and monitoring the hepatic fibrogenic process by means of IGF2R-specific peptides designed in silico
Weber, Florian; Casalini, Tommaso; Valentino, Gina; et al. (2021)
Journal of Materials Chemistry B
The lack of accurate and easily applicable methods for the diagnosis of liver fibrosis, a disease characterized by an accumulation of the extracellular matrix released by activated hepatic stellate cells (HSCs), has been a major limitation for the clinical management of liver diseases. The identification of biomarkers specific to liver microstructure alterations, combined with a non-invasive optical imaging modality, could guide clinicians towards a therapeutic strategy. In this study, structural information of the insulin-like growth factor 2 receptor (IGF2R), an overexpressed protein on activated HSCs, was used for in silico screening of novel IGF2R-specific peptide ligands. Molecular dynamics simulations, followed by computational alanine scanning of the IGF2R/IGF2 complex, led to the identification of a putative peptide sequence containing the most relevant amino acids for the receptor–ligand interaction (IGF2 E12-C21). The Residue Scan tool, implemented in the MOE software, was then used to optimize the binding affinity of this sequence by amino acid mutations. The designed peptides and their associated scrambled sequences were fluorescently labelled and their binding affinity to LX-2 cells (model for activated human HSCs) was tested using flow cytometry and confocal microscopy. In vitro binding was verified for all sequences (KD ≤ 13.2 μM). With respect to the putative binding sequence, most mutations led to an increased affinity. All sequences have shown superior binding compared to their associated scrambled sequences. Using HPLC, all peptides were tested in vitro for their proteolytic resistance and showed a stability of ≥60% intact after 24 h at 37 °C in 50% v/v FBS. In view of their prospective diagnostic application, a comparison of binding affinity was performed in perpetuated and quiescent-like LX-2 cells. Furthermore, the IGF2R expression for different cell phenotypes was analysed by a quantitative mass spectrometric approach. Our peptides showed increased binding to the perpetuated cell state, indicating their good selectivity for the diagnostically relevant phenotype. In summary, the increased binding affinity of our peptides towards perpetuated LX-2 cells, as well as the satisfactory proteolytic stability, proves that the in silico designed sequences offer a new potential strategy for the targeting of hepatic fibrosis.
Co-immobilization of enzymes with the help of a dendronized polymer and mesoporous silica nanoparticles
Gustafsson, Hanna; Küchler, Andreas; Holmberg, Krister; et al. (2015)
Journal of Materials Chemistry B
Biomimetic self-assembly of recombinant marine snail egg capsule proteins into structural coiled-coil units
Fu, Tianpei; Guerette, Paul A.; Tan, Raymond Y.T.; et al. (2015)
Journal of Materials Chemistry B

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