Journal: Developmental Cell

Abbreviation

Dev Cell

Publisher

Cell Press

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ISSN

1534-5807
1878-1551

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Publications 1 - 10 of 61
  • Waves of regulated protein expression and phosphorylation rewire the proteome to drive gametogenesis in budding yeast
    Item type: Journal Article
    Wettstein, Rahel; Hugener, Jannik; Gillet, Ludovic; et al. (2024)
    Developmental Cell
    Sexually reproducing eukaryotes employ a developmentally regulated cell division program—meiosis—to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a proteomic census encompassing the entire meiotic program of budding yeast. We found that concerted waves of protein expression and phosphorylation modify nearly all cellular pathways to support meiotic entry, meiotic progression, and gamete morphogenesis. Leveraging this comprehensive resource, we pinpointed dynamic changes in mitochondrial components and showed that phosphorylation of the FoF1-ATP synthase complex is required for efficient gametogenesis. Furthermore, using cryoET as an orthogonal approach to visualize mitochondria, we uncovered highly ordered filament arrays of Ald4ALDH2, a conserved aldehyde dehydrogenase that is highly expressed and phosphorylated during meiosis. Notably, phosphorylation-resistant mutants failed to accumulate filaments, suggesting that phosphorylation regulates context-specific Ald4ALDH2 polymerization. Overall, this proteomic census constitutes a broad resource to guide the exploration of the unique sequence of events underpinning gametogenesis.
  • Genotoxic Agents: An Unexpected Effect on Healthy Epithelia
    Item type: Journal Article
    Cangkrama, Michael; Werner, Sabine (2020)
    Developmental Cell
    DNA cross-linking agents are common chemotherapeutics for cancer treatment, but their effect on normal cells is largely unknown. In this issue of Developmental Cell, Seldin and Macara (2020) show that such compounds induce epithelial hyperplasia and stem cell fate mis-specification in a non-cell-autonomous manner via inflammasome activation in dermal fibroblasts. © 2020 Elsevier
  • EMP1-positive cells found guilty of metastatic relapse in colorectal cancer
    Item type: Journal Article
    Gvozdenovic, Ana; Aceto, Nicola (2022)
    Developmental Cell
    Metastatic recurrence develops in 30%-40% of colorectal cancer (CRC) patients in the years that follow surgical removal of the primary tumor. In a recent issue of Nature, Cañellas-Socias et al. identify a distinct population of CRC cells, marked with epithelial membrane protein 1 (EMP1), accountable for metastatic relapse.
  • GLUD1 determines murine muscle stem cell fate by controlling mitochondrial glutamate levels
    Item type: Journal Article
    Soro-Arnáiz, Inés; Fitzgerald, Gillian; Cherkaoui, Sarah; et al. (2024)
    Developmental Cell
    Muscle stem cells (MuSCs) enable muscle growth and regeneration after exercise or injury, but how metabolism controls their regenerative potential is poorly understood. We describe that primary metabolic changes can determine murine MuSC fate decisions. We found that glutamine anaplerosis into the tricarboxylic acid (TCA) cycle decreases during MuSC differentiation and coincides with decreased expression of the mitochondrial glutamate deaminase GLUD1. Deletion of Glud1 in proliferating MuSCs resulted in precocious differentiation and fusion, combined with loss of self-renewal in vitro and in vivo. Mechanistically, deleting Glud1 caused mitochondrial glutamate accumulation and inhibited the malate-aspartate shuttle (MAS). The resulting defect in transporting NADH-reducing equivalents into the mitochondria induced compartment-specific NAD+/NADH ratio shifts. MAS activity restoration or directly altering NAD+/NADH ratios normalized myogenesis. In conclusion, GLUD1 prevents deleterious mitochondrial glutamate accumulation and inactivation of the MAS in proliferating MuSCs. It thereby acts as a compartment-specific metabolic brake on MuSC differentiation.
  • A Cellular System for Spatial Signal Decoding in Chemical Gradients
    Item type: Journal Article
    Hegemann, Björn; Unger, Michael; Lee, Sung Sik; et al. (2015)
    Developmental Cell
  • Cooperation between melanoma cell states promotes metastasis through heterotypic cluster formation
    Item type: Journal Article
    Campbell, Nathaniel R.; Sznurkowska, Magdalena K.; Saini, Massimo; et al. (2021)
    Developmental Cell
    Melanomas can have multiple coexisting cell states, including proliferative (PRO) versus invasive (INV) subpopulations that represent a “go or grow” trade-off; however, how these populations interact is poorly understood. Using a combination of zebrafish modeling and analysis of patient samples, we show that INV and PRO cells form spatially structured heterotypic clusters and cooperate in the seeding of metastasis, maintaining cell state heterogeneity. INV cells adhere tightly to each other and form clusters with a rim of PRO cells. Intravital imaging demonstrated cooperation in which INV cells facilitate dissemination of less metastatic PRO cells. We identified the TFAP2 neural crest transcription factor as a master regulator of clustering and PRO/INV states. Isolation of clusters from patients with metastatic melanoma revealed a subset with heterotypic PRO-INV clusters. Our data suggest a framework for the co-existence of these two divergent cell populations, in which heterotypic clusters promote metastasis via cell-cell cooperation.
  • SrGAP2-Dependent Integration of Membrane Geometry and Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of Locomotion
    Item type: Journal Article
    Fritz, Rafael D.; Menshykau, Denis; Martin, Katrin; et al. (2015)
    Developmental Cell
  • A Hierarchical Regulatory Landscape during the Multiple Stages of EMT
    Item type: Journal Article
    Meyer-Schaller, Nathalie; Cardner, Mathias; Diepenbruck, Maren; et al. (2019)
    Developmental Cell
  • The BAR Domain Protein Arfaptin-1 Controls Secretory Granule Biogenesis at the trans-Golgi Network
    Item type: Journal Article
    Gehart, Helmuth; Goginashvili, Alexander; Beck, Rainer; et al. (2012)
    Developmental Cell
  • Control of Cell Shape, Neurite Outgrowth, and Migration by a Nogo-A/HSPG Interaction
    Item type: Journal Article
    Kempf, Anissa; Boda, Enrica; Kwok, Jessica C.F.; et al. (2017)
    Developmental Cell
Publications 1 - 10 of 61