Biomicrofluidics

Journal: Biomicrofluidics

Publisher

American Institute of Physics

ISSN

1932-1058

Show all metadata

Search Results

Publications 1 - 10 of 17

Droplet confinement and leakage: Causes, underlying effects, and amelioration strategies
Debon, Aaron P.; Wootton, Robert C.R.; Elvira, Katherine S. (2015)
Biomicrofluidics
The applicability of droplet-based microfluidic systems to many research fields stems from the fact that droplets are generally considered individual and self-contained reaction vessels. This study demonstrates that, more often than not, the integrity of droplets is not complete, and depends on a range of factors including surfactant type and concentration, the micro-channel surface, droplet storage conditions, and the flow rates used to form and process droplets. Herein, a model microfluidic device is used for droplet generation and storage to allow the comparative study of forty-four different oil/surfactant conditions. Assessment of droplet stability under these conditions suggests a diversity of different droplet failure modes. These failure modes have been classified into families depending on the underlying effect, with both numerical and qualitative models being used to describe the causative effect and to provide practical solutions for droplet failure amelioration in microfluidic systems.
Simple microfluidic stagnation point flow geometries
Dockx, Greet; Verwijlen, Tom; Sempels, Wouter; et al. (2016)
Biomicrofluidics
“Learning on a chip:” Microfluidics for formal and informal science education
Rackus, Darius George; Riedel-Kruse, Ingmar H.; Pamme, Nicole (2019)
Biomicrofluidics
Harnessing the power of Microscale AcoustoFluidics: A perspective based on BAW cancer diagnostics
Harshbarger, Cooper (2024)
Biomicrofluidics
Cancer directly affects one in every three people, and mortality rates strongly correlate with the stage at which diagnosis occurs. Each of the multitude of methods used in cancer diagnostics has its own set of advantages and disadvantages. Two common drawbacks are a limited information value of image based diagnostic methods and high invasiveness when opting for methods that provide greater insight. Microfluidics offers a promising avenue for isolating circulating tumor cells from blood samples, offering high informational value at predetermined time intervals while being minimally invasive. Microscale AcoustoFluidics, an active method capable of manipulating objects within a fluid, has shown its potential use for the isolation and measurement of circulating tumor cells, but its full potential has yet to be harnessed. Extensive research has focused on isolating single cells, although the significance of clusters should not be overlooked and requires attention within the field. Moreover, there is room for improvement by designing smaller and automated devices to enhance user-friendliness and efficiency as illustrated by the use of bulk acoustic wave devices in cancer diagnostics. This next generation of setups and devices could minimize streaming forces and thereby enable the manipulation of smaller objects, thus aiding in the implementation of personalized oncology for the next generation of cancer treatments.
Parallel study of transient dosing of antibiotics in a microfluidic device
Rackus, Darius George; Jusková, Petra; Yokoyama, Fumiaki; et al. (2022)
Biomicrofluidics
Microfluidic tools are well suited for studying bacteria as they enable the analysis of small colonies or single cells. However, current techniques for studying bacterial response to antibiotics are largely limited to static dosing. Here, we describe a microfluidic device and a method for entrapping and cultivating bacteria in hydrogel plugs. Ring-shaped isolation valves are used to define the shape of the plugs and also to control exposure of the plugs to the surrounding medium. We demonstrate bacterial cultivation, determination of the minimum inhibitory concentration of an antibiotic, and transient dosing of an antibiotic at sub-1-h doses. The transient dosing experiments reveal that at dose durations on the order of minutes, ampicillin's bactericidal effect has both a time and concentration dependency. Published under an exclusive license by AIP Publishing.
Enhanced single-cell printing by acoustophoretic cell focusing
Leibacher, Ivo; Schoendube, Jonas; Dual, Jürg; et al. (2015)
Biomicrofluidics
Artificial helical microswimmers with mastigoneme-inspired appendages
Tottori, Soichiro; Nelson, Bradley J. (2013)
Biomicrofluidics
Microfluidic-assisted fiber production: Potentials, limitations, and prospects
Abrishamkar, Afshin; Nilghaz, Azadeh; Saadatmand, Maryam; et al. (2022)
Biomicrofluidics
Besides the conventional fiber production methods, microfluidics has emerged as a promising approach for the engineered spinning of fibrous materials and offers excellent potential for fiber manufacturing in a controlled and straightforward manner. This method facilitates low-speed prototype synthesis of fibers for diverse applications while providing superior control over reaction conditions, efficient use of precursor solutions, reagent mixing, and process parameters. This article reviews recent advances in microfluidic technology for the fabrication of fibrous materials with different morphologies and a variety of properties aimed at various applications. First, the basic principles, as well as the latest developments and achievements of microfluidic-based techniques for fiber production, are introduced. Specifically, microfluidic platforms made of glass, polymers, and/or metals, including but not limited to microfluidic chips, capillary-based devices, and three-dimensional printed devices are summarized. Then, fiber production from various materials, such as alginate, gelatin, silk, collagen, and chitosan, using different microfluidic platforms with a broad range of cross-linking agents and mechanisms is described. Therefore, microfluidic spun fibers with diverse diameters ranging from submicrometer scales to hundreds of micrometers and structures, such as cylindrical, hollow, grooved, flat, core–shell, heterogeneous, helical, and peapod-like morphologies, with tunable sizes and mechanical properties are discussed in detail. Subsequently, the practical applications of microfluidic spun fibers are highlighted in sensors for biomedical or optical purposes, scaffolds for culture or encapsulation of cells in tissue engineering, and drug delivery. Finally, different limitations and challenges of the current microfluidic technologies, as well as the future perspectives and concluding remarks, are presented.
A fast and efficient size separation method for haploid embryonic stem cells
Freimann, Remo; Wutz, Anton (2017)
Biomicrofluidics
Hemizygous mutations introduced in haploid genomes can directly expose a phenotype, thus facilitating gene function analysis and forward genetic screening. Recently, mammalian haploid cells could be derived from mouse, rat, monkey, and human embryos and have been applied to screens of cellular mechanisms including cell signaling, pathogen host factors, and developmental pathways. Notably, haploid cell cultures have an intrinsic tendency for diploidization and, thus, require periodic cell sorting. Here, we report a method for rapid purification of haploid mouse embryonic stem cells from mixed cell populations with high viability and yield. Our method uses membranes with micrometer pores for force-free separation and facilitates enrichment of haploid cells without flow cytometry. The separation method simplifies maintaining haploid cell cultures and has further applications in establishing haploid cell lines from embryos and isolating cell cycle phases of mammalian cells.
Microfluidic generation of PEG-b-PLA polymersomes containing alginate-based core hydrogel
Martino, Chiara; Lee, Taeyong; Kim, Shinhyun; et al. (2015)
Biomicrofluidics

Publications 1 - 10 of 17

Journal: Biomicrofluidics

Abbreviation

Publisher

Journal Volumes

ISSN

Description

Filters

Search Results