Journal: ACS Sensors

Abbreviation

ACS Sens

Publisher

American Chemical Society

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ISSN

2379-3694

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2016 - 2019102020 - 202525
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Publications1 - 10 of 35
  • Real-Time Respiration Changes as a Viability Indicator for Rapid Antibiotic Susceptibility Testing in a Microfluidic Chamber Array
    Item type: Journal Article
    Jusková, Petra; Schmitt, Steven; Kling, André; et al. (2021)
    ACS Sensors
    Rapid identification of a pathogen and the measurement of its antibiotic susceptibility are key elements in the diagnostic process of bacterial infections. Microfluidic technologies offer great control over handling and manipulation of low sample volumes with the possibility to study microbial cultures on the single-cell level. Downscaling the dimensions of cultivation systems directly results in a lower number of bacteria required for antibiotic susceptibility testing (AST) and thus in a reduction of the time to result. The developed platform presented in this work allows the reading of pathogen resistance profiles within 2–3 h based on the changes of dissolved oxygen levels during bacterial cultivation. The platform contains hundreds of individual growth chambers prefilled with a hydrogel containing oxygen-sensing nanoprobes and different concentrations of antibiotic compounds. The performance of the developed platform is tested using quality control Escherichia coli strains (ATCC 25922 and ATCC 35218) in response to clinically relevant antibiotics. The results are in agreement with values given in reference guidelines and independent measurements using a clinical AST protocol. Finally, the platform is successfully used for the AST of an E. coli clinical isolate obtained from a patient blood culture.
  • An Approach for the Real-Time Quantification of Cytosolic Protein–Protein Interactions in Living Cells
    Item type: Journal Article
    Incaviglia, Ilaria; Frutiger, Andreas; Blickenstorfer, Yves; et al. (2021)
    ACS Sensors
    In recent years, cell-based assays have been frequently used in molecular interaction analysis. Cell-based assays complement traditional biochemical and biophysical methods, as they allow for molecular interaction analysis, mode of action studies, and even drug screening processes to be performed under physiologically relevant conditions. In most cellular assays, biomolecules are usually labeled to achieve specificity. In order to overcome some of the drawbacks associated with label-based assays, we have recently introduced “cell-based molography” as a biosensor for the analysis of specific molecular interactions involving native membrane receptors in living cells. Here, we expand this assay to cytosolic protein–protein interactions. First, we created a biomimetic membrane receptor by tethering one cytosolic interaction partner to the plasma membrane. The artificial construct is then coherently arranged into a two-dimensional pattern within the cytosol of living cells. Thanks to the molographic sensor, the specific interactions between the coherently arranged protein and its endogenous interaction partners become visible in real time without the use of a fluorescent label. This method turns out to be an important extension of cell-based molography because it expands the range of interactions that can be analyzed by molography to those in the cytosol of living cells.
  • Wearable Sensors
    Item type: Other Journal Item
    deMello, Andrew J.; Bakker, Eric (2023)
    ACS Sensors
  • Electrical Impedance Spectroscopy for Microtissue Spheroid Analysis in Hanging-Drop Networks
    Item type: Journal Article
    Schmid, Yannick R.F.; Bürgel, Sebastian C.; Misun, Patrick M.; et al. (2016)
    ACS Sensors
  • Investigating Complex Samples with Molograms of Low-Affinity Binders
    Item type: Journal Article
    Reichmuth, Andreas M.; Kübrich, Katharina; Blickenstorfer, Yves; et al. (2021)
    ACS Sensors
    In vitro diagnostics relies on the quantification of minute amounts of a specific biomolecule, called biomarker, from a biological sample. The majority of clinically relevant biomarkers for conditions beyond infectious diseases are detected by means of binding assays, where target biomarkers bind to a solid phase and are detected by biochemical or physical means. Nonspecifically bound biomolecules, the main source of variation in such assays, need to be washed away in a laborious process, restricting the development of widespread point-of-care diagnostics. Here, we show that a diffractometric assay provides a new, label-free possibility to investigate complex samples, such as blood plasma. A coherently arranged sub-micron pattern, that is, a peptide mologram, is created to demonstrate the insensitivity of this diffractometric assay to the unwanted masking effect of nonspecific interactions. In addition, using an array of low-affinity binders, we also demonstrate the feasibility of molecular profiling of blood plasma in real time and show that individual patients can be differentiated based on the binding kinetics of circulating proteins.
  • Highly Selective and Rapid Breath Isoprene Sensing Enabled by Activated Alumina Filter
    Item type: Journal Article
    van den Broek, Jan; Güntner, Andreas; Pratsinis, Sotiris E. (2018)
    ACS Sensors
  • Parallelized Wireless Sensing System for Continuous Monitoring of Microtissue Spheroids
    Item type: Journal Article
    Dong, Lei; Ravaynia, Paolo S.; Huang, Qing-An; et al. (2020)
    ACS Sensors
    Currently, the use of electrical readout methods for the investigation of microtissue spheroids in combination with lab automation tools is hindered by the cable connections that are required to interrogate the on-chip-integrated electrodes. To overcome this limitation, we developed a wireless sensor scheme, which can detect the size variation of microtissues during long-term culturing and drug exposure assays. The sensor system includes an interrogation board, which is composed of an inductor-capacitor (LC) readout circuit, and the tissue culture platform with integrated split-ring sensors. The magnetic coupling between the LC circuit and the sensors enables the interrogation of the on-chip sensors without any wire connection to the culture platform. By optimizing the sensor dimensions and the LC resonance frequencies, we were able to avoid cross talk between neighboring sensors. We integrated 12 tissue compartments on a standard microscopy slide with a sensor-to-sensor pitch of 9 mm, which is in accordance with standard 96-well plate dimensions. As a proof-of-concept experiment for the developed system, we monitored continuously and during more than four days the growth inhibition of colon cancer microtissue spheroids that had been exposed to different concentrations of doxorubicin, a chemotherapeutic compound. The stability of the measurements during long-term culturing and the compatibility of the sensor scheme with standard lab equipment offer great potential for automated electrical microtissue spheroid characterization. © 2020 American Chemical Society.
  • 2021: A Year Starting Full of Hope
    Item type: Other Journal Item
    Gooding, J. Justin; Camasso, Nicole; Bakker, Eric; et al. (2021)
    ACS Sensors
  • Characterization of Single Yeast Cell Phenotypes Using Microfluidic Impedance Cytometry and Optical Imaging
    Item type: Journal Article
    Haandbaek, Niels; Burgel, Sebastian C.; Rudolf, Fabian; et al. (2016)
    ACS Sensors
  • Dark-Field Microwells toward High-Throughput Direct miRNA Sensing with Gold Nanoparticles
    Item type: Journal Article
    Hwu, Stephanie; Blickenstorfer, Yves; Tiefenauer, Raphael F.; et al. (2019)
    ACS Sensors
Publications1 - 10 of 35