Biotechnology Journal

Journal: Biotechnology Journal

Abbreviation

Biotechnol. J.

Publisher

Wiley-VCH

ISSN

1860-7314
1860-6768

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Publications 1 - 10 of 17

Sequential multivariate cell culture modeling at multiple scales supports systematic shaping of a monoclonal antibody towards a quality target
Sokolov, Michael; Morbidelli, Massimo; Butté, Alessandro; et al. (2018)
Biotechnology Journal
Novel calibration design improves knowledge transfer across products for the characterization of pharmaceutical bioprocesses
Helleckes, Laura M.; Wirnsperger, Claus; Polak, Jakub; et al. (2024)
Biotechnology Journal
Modern machine learning has the potential to fundamentally change the way bioprocesses are developed. In particular, horizontal knowledge transfer methods, which seek to exploit data from historical processes to facilitate process development for a new product, provide an opportunity to rethink current workflows. In this work, we first assess the potential of two knowledge transfer approaches, meta learning and one-hot encoding, in combination with Gaussian process (GP) models. We compare their performance with GPs trained only on data of the new process, that is, local models. Using simulated mammalian cell culture data, we observe that both knowledge transfer approaches exhibit test set errors that are approximately halved compared to those of the local models when two, four, or eight experiments of the new product are used for training. Subsequently, we address the question whether experiments for a new product could be designed more effectively by exploiting existing knowledge. In particular, we suggest to specifically design a few runs for the novel product to calibrate knowledge transfer models, a task that we coin calibration design. We propose a customized objective function to identify a set of calibration design runs, which exploits differences in the process evolution of historical products. In two simulated case studies, we observed that training with calibration designs yields similar test set errors compared to common design of experiments approaches. However, the former requires approximately four times fewer experiments. Overall, the results suggest that process development could be significantly streamlined when systematically carrying knowledge from one product to the next.
Resistance to agricultural biotechnology: The importance of distinguishing between weak and strong public attitudes
Aerne, Philipp (2013)
Biotechnology Journal
Improved Performance in Mammalian Cell Perfusion Cultures by Growth Inhibition
Wolf, Moritz K.F.; Closet, Aurélie; Bzowska, Monika; et al. (2019)
Biotechnology Journal
Continuous counter-current chromatography for capture and polishing steps in biopharmaceutical production
Steinebach, Fabian; Müller-Späth, Thomas; Morbidelli, Massimo (2016)
Biotechnology Journal
Mammalian designer cells: Engineering principles and biomedical applications
Xie, Mingqi; Fussenegger, Martin (2015)
Biotechnology Journal
Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses
Paganini, Carolina; Capasso Palmiero, Umberto; Pocsfalvi, Gabriella; et al. (2019)
Biotechnology Journal
Comparison of batch and continuous multi-column protein A capture processes by optimal design
Baur, Daniel; Angarita, Monica; Müller-Späth, Thomas; et al. (2016)
Biotechnology Journal
Simultaneous Repression of GLUCAN WATER DIKINASE 1 and STARCH BRANCHING ENZYME 1 in Potato Tubers Leads to Starch With Increased Amylose and Novel Industrial Properties
Adegbaju, Muyiwa S.; Gouws, Nina; van der Vyver, Christell; et al. (2025)
Biotechnology Journal
This study examines how post-transcriptional gene silencing of STARCH BRANCHING ENZYME 1 (SBE1) and GLUCAN WATER DIKINASE 1 (GWD1) affects the structure and properties of potato tuber starch. Silencing of either gene individually or simultaneously altered starch chemistry physical properties. Repression of StGWD1 reduced phosphate content, while repression of StSBE1 increased it. The phosphate content of starch isolated from plants where both genes were repressed was increased compared to StGWD1 repressed lines, but lower than both the SBE1 repressed lines and the untransformed control. Constituent chain lengths of starches from all lines were altered, and amylose content was increased in the gwd1 and sbe1/gwd1 double repressed lines, which also accumulated small numbers of lobed starch granules. Pasting properties were also affected, with starch from StSBE1-repressed lines demonstrating increased peak and trough viscosities and gwd1 lines showing decreased peak and trough viscosities, compared with the control. Peak and trough viscosities were lowest in the sbe1/gwd1 repressed lines. We believe that these data demonstrate that alterations in starch phosphate influence the degree of branching within starch and offer a novel in planta strategy for optimizing the industrial properties of potato storage starch.
Growth and accumulation dynamics of poly(3-hydroxyalkanoate) (PHA) in Pseudomonas putida GPo1 cultivated in continuous culture under transient feed conditions
Zinn, Manfred; Durner, Roland; Zinn, Hanspeter; et al. (2011)
Biotechnology Journal

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Journal: Biotechnology Journal

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