The FEBS Journal

Journal: The FEBS Journal

Abbreviation

FEBS j.

Publisher

Wiley-Blackwell

ISSN

1742-464X
1742-4658

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Publications 1 - 10 of 80

Putative prion protein from Fugu (Takifugu rubripes)
Christen, Barbara; Wüthrich, Kurt; Hornemann, Simone (2008)
The FEBS Journal
Cell-free expression of disulfide-containing eukaryotic proteins for structural biology
Michel, Erich; Wüthrich, Kurt (2012)
The FEBS Journal
Design and preparation of the class B G protein-coupled receptors GLP-1R and GCGR for 19F-NMR studies in solution
Wang, Huixia; Hu, Wanhui; Liu, Dongsheng; et al. (2021)
The FEBS Journal
The human glucagon-like peptide-1 receptor (GLP-1R) and the glucagon receptor (GCGR) are class B G protein-coupled receptors (GPCRs) that are activated by interactions with, respectively, the glucagon-like peptide-1 (GLP-1) and glucagon (GCG). These polypeptide hormones are involved in the regulation of lipid and cholic acid metabolism, and thus play an important role in the pathogenesis of glucose metabolism and diabetes mellitus, which attracts keen interest of these GPCRs as drug targets. GLP-1R and GCGR have therefore been extensively investigated by X-ray crystallography and cryo-electron microscopy (cryo-EM), so that their structures are well known. Here, we present the groundwork for using nuclear magnetic resonance (NMR) spectroscopy in solution to complement the molecular architectures with information on intramolecular dynamics and on the thermodynamics and kinetics of interactions with physiological ligands and extrinsic drug candidates. This includes the generation of novel, near-wild-type constructs of GLP-1R and GCGR, optimization of the solution conditions for NMR studies in detergent micelles and in nanodiscs, post-translational chemical introduction of fluorine-19 NMR probes, and sequence-specific assignments of the 19F-labels attached to indigenous cysteines. Addition of the negative allosteric modulator (NAM) NNC0640 was critically important for obtaining the long-time stability needed for our NMR experiments, and we report on novel insights into the allosteric effects arising from binding of NNC0640 to the transmembrane domain of GLP-1R (GLP-1R[TMD]).
Prion protein library of recombinant constructs for structural biology
Hornemann, Simone; Christen, Barbara; von Schroetter, Christine; et al. (2009)
The FEBS Journal
Unusual modes of RNA recognitions by RNA recognition motifs
Afroz, Tariq; Cléry, Antoine; Allain, Frédéric H.-T. (2012)
The FEBS Journal
Amyloid fibrils
Johansson, Jan (2005)
The FEBS Journal
Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type ISPase
Catrein, Ina; Herrmann, Richard; Bosserhoff, Armin; et al. (2005)
The FEBS Journal
Quantitative proteomics and systems biology
Aebersold, Ruedi (2006)
The FEBS Journal
Distinct actin oligomers modulate differently the activity of actin nucleators
Qu, Zheng; Silvan, Unai; Jockusch, Brigitte M.; et al. (2015)
The FEBS Journal
Quantitative proteomics and systems biology
Aebersold, Ruedi (2005)
The FEBS Journal

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