Journal: Nature Protocols
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Abbreviation
Nat Protoc
Publisher
Nature
68 results
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Publications 1 - 10 of 68
- Quantification of starch in plant tissuesItem type: Journal Article
Nature ProtocolsSmith, Alison M.; Zeeman, Samuel C. (2006) - Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopyItem type: Journal Article
Nature ProtocolsFriedrichs, Jens; Helenius, Jonne; Muller, Daniel J. (2010) - Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeastItem type: Journal Article
Nature ProtocolsKaplan, Charlotte; Ewers, Helge (2015) - Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometryItem type: Journal Article
Nature ProtocolsSchopper, Simone; Kahraman, Abdullah; Leuenberger, Pascal; et al. (2017) - Reversible DNA encapsulation in silica to produce ROS-resistant and heat-resistant synthetic DNA 'fossils'Item type: Journal Article
Nature ProtocolsPaunescu, Daniela; Puddu, Michela; Soellner, Justus O. B.; et al. (2013) - A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodentsItem type: Journal Article
Nature ProtocolsPrivitera, Mattia; Ferrari, Kim D.; von Ziegler, Lukas; et al. (2020)The locus coeruleus (LC) is a region in the brainstem that produces noradrenaline and is involved in both normal and pathological brain function. Pupillometry, the measurement of pupil diameter, provides a powerful readout of LC activity in rodents, primates and humans. The protocol detailed here describes a miniaturized setup that can screen LC activity in rodents in real-time and can be established within 1–2 d. Using low-cost Raspberry Pi computers and cameras, the complete custom-built system costs only ~300 euros, is compatible with stereotaxic surgery frames and seamlessly integrates into complex experimental setups. Tools for pupil tracking and a user-friendly Pupillometry App allow quantification, analysis and visualization of pupil size. Pupillometry can discriminate between different, physiologically relevant firing patterns of the LC and can accurately report LC activation as measured by noradrenaline turnover. Pupillometry provides a rapid, non-invasive readout that can be used to verify accurate placement of electrodes/fibers in vivo, thus allowing decisions about the inclusion/exclusion of individual animals before experiments begin. - Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometryItem type: Journal Article
Nature ProtocolsShiio, Yuzuru; Aebersold, Ruedi (2006) - Solid-phase synthesis, cyclization, and site-specific functionalization of aziridine-containing tetrapeptidesItem type: Journal Article
Nature ProtocolsChung, Benjamin K. W.; White, Christopher J.; Yudin, Andrei K. (2017) - Automated selected reaction monitoring data analysis workflow for large-scale targeted proteomic studiesItem type: Journal Article
Nature ProtocolsSurinova, Silvia; Hüttenhain, Ruth; Chang, Ching-Yun; et al. (2013) - Solid-phase extraction of N-linked glycopeptidesItem type: Journal Article
Nature ProtocolsTian, Yuan; Zhou, Yong; Elliott, Sarah; et al. (2007)
Publications 1 - 10 of 68