Nature Protocols

Journal: Nature Protocols

Abbreviation

Nat Protoc

Publisher

Nature

ISSN

1750-2799
1754-2189

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Publications 1 - 10 of 68

Quantification of starch in plant tissues
Smith, Alison M.; Zeeman, Samuel C. (2006)
Nature Protocols
Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy
Friedrichs, Jens; Helenius, Jonne; Muller, Daniel J. (2010)
Nature Protocols
Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast
Kaplan, Charlotte; Ewers, Helge (2015)
Nature Protocols
Measuring protein structural changes on a proteome-wide scale using limited proteolysis-coupled mass spectrometry
Schopper, Simone; Kahraman, Abdullah; Leuenberger, Pascal; et al. (2017)
Nature Protocols
Reversible DNA encapsulation in silica to produce ROS-resistant and heat-resistant synthetic DNA 'fossils'
Paunescu, Daniela; Puddu, Michela; Soellner, Justus O. B.; et al. (2013)
Nature Protocols
A complete pupillometry toolbox for real-time monitoring of locus coeruleus activity in rodents
Privitera, Mattia; Ferrari, Kim D.; von Ziegler, Lukas; et al. (2020)
Nature Protocols
The locus coeruleus (LC) is a region in the brainstem that produces noradrenaline and is involved in both normal and pathological brain function. Pupillometry, the measurement of pupil diameter, provides a powerful readout of LC activity in rodents, primates and humans. The protocol detailed here describes a miniaturized setup that can screen LC activity in rodents in real-time and can be established within 1–2 d. Using low-cost Raspberry Pi computers and cameras, the complete custom-built system costs only ~300 euros, is compatible with stereotaxic surgery frames and seamlessly integrates into complex experimental setups. Tools for pupil tracking and a user-friendly Pupillometry App allow quantification, analysis and visualization of pupil size. Pupillometry can discriminate between different, physiologically relevant firing patterns of the LC and can accurately report LC activation as measured by noradrenaline turnover. Pupillometry provides a rapid, non-invasive readout that can be used to verify accurate placement of electrodes/fibers in vivo, thus allowing decisions about the inclusion/exclusion of individual animals before experiments begin.
Quantitative proteome analysis using isotope-coded affinity tags and mass spectrometry
Shiio, Yuzuru; Aebersold, Ruedi (2006)
Nature Protocols
Solid-phase synthesis, cyclization, and site-specific functionalization of aziridine-containing tetrapeptides
Chung, Benjamin K. W.; White, Christopher J.; Yudin, Andrei K. (2017)
Nature Protocols
Automated selected reaction monitoring data analysis workflow for large-scale targeted proteomic studies
Surinova, Silvia; Hüttenhain, Ruth; Chang, Ching-Yun; et al. (2013)
Nature Protocols
Solid-phase extraction of N-linked glycopeptides
Tian, Yuan; Zhou, Yong; Elliott, Sarah; et al. (2007)
Nature Protocols

Publications 1 - 10 of 68

Journal: Nature Protocols

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