Journal: Cellular Microbiology

Abbreviation

Cell Microbiol

Publisher

Wiley

Journal Volumes

ISSN

1462-5814
1462-5822

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2013 - 201952020 - 20213
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Publications1 - 8 of 8
  • The Salmonella Typhimurium effector protein SopE transiently localizes to the early SCV and contributes to intracellular replication.
    Item type: Journal Article
    Vonaesch, Pascale; Sellin, Mikael E.; Cardini, Steven; et al. (2014)
    Cellular Microbiology
  • Intestinal inflammation alters mucosal carbohydrate foraging and monosaccharide incorporation into microbial glycans
    Item type: Journal Article
    Weiss, Gisela A.; Grabinger, Thomas; Glaus Garzon, Jesus; et al. (2021)
    Cellular Microbiology
    Endogenous carbohydrates released from the intestinal mucus represent a constant source of nutrients to the intestinal microbiota. Mucus-derived carbohydrates can also be used as building blocks in the biosynthesis of bacterial cell wall components, thereby influencing host mucosal immunity. To assess the uptake of endogenous carbohydrates by gut microbes in healthy mice and during intestinal inflammation, we applied azido-monosaccharides that can be tracked on bacterial cell walls after conjugation with fluorophores. In interleukin-10 deficient mice, changes in the gut microbiota were accompanied by decreased carbohydrate hydrolase activities and increased lumenal concentrations of host glycan-derived monosaccharides. Tracking of the monosaccharideN-azidoacetylglucosamine (GlcNAz) in caecum bacteria revealed a preferential incorporation of this carbohydrate byXanthomonadaceaein healthy mice and byBacteroidaceaein interleukin-10 deficient mice. These GlcNAz-positiveBacteroidaceaefractions mainly belonged to the speciesB. acidifaciensandB. vulgatus. Growth ofBacteroidesspecies in the presence of specific monosaccharides changed their stimulatory activity toward CD11c(+)dendritic cells. Expression of activation markers and cytokine production was highest after stimulation of dendritic cells withB. vulgatus. The variable incorporation of monosaccharides by relatedBacteroidesspecies underline the necessity to investigate intestinal bacteria down to the species level when addressing microbiota-host interactions.
  • A P4-ATPase subunit of the Cdc50 family plays a role in iron acquisition and virulence in Cryptococcus neoformans
    Item type: Journal Article
    Hu, Guanggan; Caza, Mélissa; Bakkeren, Erik; et al. (2017)
    Cellular Microbiology
  • Low-oxygen tensions found in Salmonella-infected gut tissue boost Salmonella replication in macrophages by impairing antimicrobial activity and augmenting Salmonella virulence
    Item type: Journal Article
    Jennewein, Jonas; Matuszak, Jasmin; Walter, Steffi; et al. (2015)
    Cellular Microbiology
  • The infectious propagules of Aspergillus fumigatus are coated with antimicrobial peptides
    Item type: Journal Article
    Dümig, Michaela; Binder, Jasmin; Gaculenko, Anastasia; et al. (2021)
    Cellular Microbiology
    Fungal spores are unique cells that mediate dispersal and survival in the environment. For pathogenic fungi encountering a susceptible host, these specialised structures may serve as infectious particles. The main causative agent of the opportunistic disease aspergillosis, Aspergillus fumigatus, produces asexual spores, the conidia, that become dissipated by air flows or water currents but also serve as propagules to infect a susceptible host. We demonstrate that the defX gene of this mould encodes putative antimicrobial peptides resembling cysteine‐stabilised (CS)αβ defensins that are expressed in a specific spatial and temporal manner in the course of asexual spore formation. Localisation studies on strains expressing a fluorescent proxy or tagged defX alleles expose that these antimicrobial peptides are secreted to coat the conidial surface. Deletion mutants reveal that the spore‐associated defX gene products delay the growth of Gram‐positive Staphylococcus aureus and demonstrate that the defX gene and presumably its encoded spore‐associated defensins confer a growth advantage to the fungal opponent over bacterial competitors. These findings have implications with respect to the ecological niche of A. fumigatus that serves as a ‘virulence school’ for this human pathogenic mould; further relevance is given for the infectious process resulting in aspergillosis, considering competition with the host microbiome or co‐infecting microorganisms to break colonisation resistance at host surfaces.
  • Actin ADP-ribosylation at Threonine148 by Photorhabdus luminescens toxin TccC3 induces aggregation of intracellular F-actin
    Item type: Journal Article
    Lang, Alexander E.; Qu, Zheng; Schwan, Carsten; et al. (2017)
    Cellular Microbiology
  • Quantitative insights into actin rearrangements and bacterial target site selection from Salmonella Typhimurium infection of micropatterned cells
    Item type: Journal Article
    Vonaesch, Pascale; Cardini, Steven; Sellin, Mikael E.; et al. (2013)
    Cellular Microbiology
  • Germ‐free and microbiota‐associated mice yield small intestinal epithelial organoids with equivalent and robust transcriptome/proteome expression phenotypes
    Item type: Journal Article
    Hausmann, Annika; Russo, Giancarlo; Grossmann, Jonas; et al. (2020)
    Cellular Microbiology
    Intestinal epithelial organoids established from gut tissue have become a widely used research tool. However, it remains unclear how environmental cues, divergent microbiota composition and other sources of variation before, during and after establishment confound organoid properties, and how these properties relate to the original tissue. While environmental influences cannot be easily addressed in human organoids, mice offer a controlled assay‐system. Here, we probed the effect of donor microbiota differences, previously identified as a confounding factor in murine in vivo studies, on organoids. We analysed the proteomes and transcriptomes of primary organoid cultures established from two colonised and one germ‐free mouse colony of C57BL/6J genetic background, and compared them to their tissue of origin and commonly used cell lines. While an imprint of microbiota‐exposure was observed on the proteome of epithelial samples, the long‐term global impact of donor microbiota on organoid expression patterns was negligible. Instead, stochastic culture‐to‐culture differences accounted for a moderate variability between independently established organoids. Integration of transcriptome and proteome datasets revealed an organoid‐typic expression signature comprising 14 transcripts and 10 proteins that distinguished organoids across all donors from murine epithelial cell lines and fibroblasts and closely mimicked expression patterns in the gut epithelium. This included the inflammasome components ASC, Naip1‐6, Nlrc4 and Caspase‐1, which were highly expressed in all organoids compared to the reference cell line m‐ICc12 or mouse embryonic fibroblasts. Taken together, these results reveal that the donor microbiota has little effect on the organoid phenotype and suggest that organoids represent a more suitable culture model than immortalised cell lines, in particular for studies of intestinal epithelial inflammasomes.
Publications1 - 8 of 8