The Journal of Gene Medicine

Journal: The Journal of Gene Medicine

Abbreviation

J. gene med.

Publisher

Wiley

ISSN

1099-498X
1521-2254

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Publications 1 - 10 of 11

A novel binary adenovirus-based dual-regulated expression system for independent transcription control of two different transgenes
Gonzalez-Nicolini, Valeria; Fussenegger, Martin (2005)
The Journal of Gene Medicine
Adenoviral vector platform for transduction of constitutive and regulated tricistronic or triple-transcript transgene expression in mammalian cells and microtissues
Gonzalez-Nicolini, Valeria; Sanchez-Bustamante, Carlota D.; Hartenbach, Shizuka; et al. (2006)
The Journal of Gene Medicine
Contribution of the lysosomal cysteine proteinase cathepsin B to extracellular matrix remodelling during keratinocyte migration
Bueth, Heiko; Fusenig, Norbert; Werner, Sabine; et al. (2003)
The Journal of Gene Medicine
Engineered Streptomyces quorum-sensing components enable inducible siRNA-mediated translation control in mammalian cells and adjustable transcription control in mice
Weber, Wilfried; Malphettes, Laetitia; de Jesus, Maria; et al. (2005)
The Journal of Gene Medicine
Novel macrolide-adjustable bidirectional expression modules for coordinated expression of two different transgenes in mice
Baessler, Cornelia; Weber, Wilfried; Baba, Marie Daoud-El; et al. (2003)
The Journal of Gene Medicine
Generation and characterization of tTS-H4: a novel transcriptional repressor that is compatible with the reverse tetracycline-controlled TET-ON system
Bockamp, Ernesto; Christel, Cerstin; Hameyer, Dorothe; et al. (2007)
The Journal of Gene Medicine
Background Conditional gene regulatory systems ensuring tight and adjustable expression of therapeutic genes are central for developing future gene therapy strategies. Among various regulatory systems, tetracyclinecontrolled gene expression has emerged as a safe and reliable option. Moreover, the tightness of tetracycline-regulated gene switches can be substantially improved by complementing transcriptional activators with antagonizing repressors. Methods To develop novel tetracycline-responsive transcriptional repressors, we fused various transcriptional silencing domains to the TetR (B/E) DNA-binding and dimerization domain of the Tn10-encoded tetracycline resistance operon (TetR (B/E)). The resulting fusion proteins were individually tested for their ability to repress transcription of the constitutively active hypoxanthine phosphoribosyltransferase (HPRT) promoter. In addition, compatibility with the commonly used reverse tetracycline-controlled transactivator system (rtTA-system) and responsiveness to the pharmacological effector doxycycline (DOX) were evaluated. Finally, inducibility, effector-dependent promoter activity and the modification of histone H3 and H4 of the active versus the repressed target promoter were determined. Results Fusion of the human deacetylase 4 (HDAC4) carboxy-terminal silencing domain to TetR (B/E) resulted in a functional transcriptional repressor. This novel repressor, termed tTS-H4, efficiently reduced the activity of the murine HPRT promoter and a constitutively active human cytomegalovirus (hCMV) minimal promoter. Furthermore, combining tTS-H4 with the rtTA transcriptional activator allowed for grading, turning off and resuming target gene expression over several orders of magnitude without background. Conclusions The tTS-H4 repressor is compatible with the commonly used rtTA transcriptional activation system and is a versatile new tool for tightly and adjustably regulating conditional gene expression.
Pharmacologic transgene control systems for gene therapy
Weber, Wilfried; Fussenegger, Martin (2006)
The Journal of Gene Medicine
In vivo transduction of HIV-1-derived lentiviral particles engineered for macrolide-adjustable transgene expression
Mitta, Barbara; Weber, Cornelia C.; Fussenegger, Martin (2005)
The Journal of Gene Medicine
Optimisation of a multipartite human immunodeficiency virus based vector system; control of virus infectivity and large-scale production
Koldej, R.; Cmielewski, P.; Stocker, A.; et al. (2005)
The Journal of Gene Medicine
Engineered human mesenchymal stem cells
Turgeman, Gadi; Pittman, Debbie D.; Müller, Ralph; et al. (2001)
The Journal of Gene Medicine

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