Journal: Development

Abbreviation

Development

Publisher

Company of Biologists

Journal Volumes

ISSN

0950-1991
1477-9129

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Publications 1 - 10 of 75
  • DeXtrusion: automatic recognition of epithelial cell extrusion through machine learning in vivo
    Item type: Journal Article
    Villars, Alexis; Letort, Gaelle; Valon, Leo; et al. (2023)
    Development
    Accurately counting and localising cellular events from movies is an important bottleneck of high-content tissue/embryo liv e imaging. Here, we propose a new methodology based on deep learning that allows automatic detection of cellular events and their precise xyt localisation on liv e fluorescent imaging movies without segmentation. We focused on the detection of cell extrusion, the expulsion of dying cells from the epithelial layer, and devised DeXtrusion: a pipeline based on recurrent neural networks for automatic detection of cell extrusion/cell death events in large movies of epithelia mar k e d with cell contour. The pipeline, initially trained on movies of the Drosophila pupal notum marked with fluorescent E-cadherin, is easily trainable, provides fast and accurate extrusion predictions in a large range of imaging conditions, and can also detect other cellular events, such as cell division or cell differentiation. It also performs well on other epithelial tissues with reasonable re-training. Our methodology could easily be applied for other cellular events detected by liv e fluorescent microscopy and could help to democratise the use of deep learning for automatic event detections in developing tissues.
  • Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to promote epidermal regeneration after injury
    Item type: Journal Article
    Mardaryev, Andrei N.; Meier, Natalia; Poterlowicz, Krzysztof; et al. (2011)
    Development
  • The biomechanical basis of biased epithelial tube elongation in lung and kidney development
    Item type: Journal Article
    Conrad, Lisa; Runser, Steve; Gómez, Harold F.; et al. (2021)
    Development
    During lung development, epithelial branches expand preferentially in a longitudinal direction. This bias in outgrowth has been linked to a bias in cell shape and in the cell division plane. How this bias arises is unknown. Here, we show that biased epithelial outgrowth occurs independent of the surrounding mesenchyme, of preferential turnover of the extracellular matrix at the bud tips and of FGF signalling. There is also no evidence for actin-rich filopodia at the bud tips. Rather, we find epithelial tubes to be collapsed during early lung and kidney development, and we observe fluid flow in the narrow tubes. By simulating the measured fluid flow inside segmented narrow epithelial tubes, we show that the shear stress levels on the apical surface are sufficient to explain the reported bias in cell shape and outgrowth. We use a cell-based vertex model to confirm that apical shear forces, unlike constricting forces, can give rise to both the observed bias in cell shapes and tube elongation. We conclude that shear stress may be a more general driver of biased tube elongation beyond its established role in angiogenesis.
  • RETINOBLASTOMA-RELATED PROTEIN controls the transition to autotrophic plant development
    Item type: Journal Article
    Gutzat, Ruben; Borghi, Lorenzo; Fütterer, Johannes; et al. (2011)
    Development
  • The impact of cell size on morphogen gradient precision
    Item type: Journal Article
    Adelmann, Jan A.; Vetter, Roman; Iber, Dagmar (2023)
    Development
    Tissue patterning during embryonic development is remarkably precise. Here, we numerically determine the impact of the cell diameter, gradient length and the morphogen source on the variability of morphogen gradients. We show that the positional error increases with the gradient length relative to the size of the morphogen source, and with the square root of the cell diameter and the readout position. We provide theoretical explanations for these relationships, and show that they enable high patterning precision over developmental time for readouts that scale with expanding tissue domains, as observed in the Drosophila wing disc. Our analysis suggests that epithelial tissues generally achieve higher patterning precision with small cross-sectional cell areas. An extensive survey of measured apical cell areas shows that they are indeed small in developing tissues that are patterned by morphogen gradients. Enhanced precision may thus have led to the emergence of pseudostratification in epithelia, a phenomenon for which the evolutionary benefit had so far remained elusive.
  • PI(4,5)P2 forms dynamic cortical structures and directs actin distribution as well as polarity in Caenorhabditis elegans embryos.
    Item type: Journal Article
    Scholze, Melina J.; Babieux, Kévin S.; De Simone, Alessandro; et al. (2018)
    Development
  • Planar cell polarity signalling regulates cell adhesion properties in progenitors of the zebrafish laterality organ
    Item type: Journal Article
    Oteiza, Pablo; Köppen, Mathias; Krieg, Michael; et al. (2010)
    Development
  • Female gametophytic cell specification and seed development require the function of the putative Arabidopsis INCENP ortholog WYRD
    Item type: Journal Article
    Kirioukhova, Olga; Johnston, Amal J.; Kleen, Daniela; et al. (2011)
    Development
  • Fibroblast growth factors: key players in regeneration and tissue repair
    Item type: Review Article
    Maddaluno, Luigi; Urwyler, Corinne; Werner, Sabine (2017)
    Development
  • SHH propagates distal limb bud development by enhancing CYP26B1-mediated retinoic acid clearance via AER-FGF signalling
    Item type: Journal Article
    Probst, Simone; Kraemer, Conradin; Demougin, Philippe; et al. (2011)
    Development
Publications 1 - 10 of 75