ACS Chemical Biology

Journal: ACS Chemical Biology

Publisher

American Chemical Society

ISSN

1554-8929
1554-8937

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Publications1 - 10 of 51

A Peptidomimetic Antibiotic Interacts with the Periplasmic Domain of LptD from Pseudomonas aeruginosa
Andolina, Gloria; Bencze, László-Csaba; Zerbe, Katja; et al. (2018)
ACS Chemical Biology
Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems
Modena, Mario M.; Chawla, Ketki; Misun, Patrick M.; et al. (2018)
ACS Chemical Biology
The Base Pairing Partner Modulates Alkylguanine Alkyltransferase
McKeague, Maureen; Otto, Claudia; Räz, Michael H.; et al. (2018)
ACS Chemical Biology
Live-Cell Fluorescence Lifetime Multiplexing Using Synthetic Fluorescent Probes
Frei, Michelle; Koch, Birgit; Hiblot, Julien; et al. (2022)
ACS Chemical Biology
Fluorescence lifetime multiplexing requires fluorescent probes with distinct fluorescence lifetimes but similar spectral properties. Even though synthetic probes for many cellular targets are available for multicolor live-cell fluorescence microscopy, few of them have been characterized for their use in fluorescence lifetime multiplexing. Here, we demonstrate that, from a panel of 18 synthetic probes, eight pairwise combinations are suitable for fluorescence lifetime multiplexing in living mammalian cell lines. Moreover, combining multiple pairs in different spectral channels enables us to image four and with the help of self-labeling protein tags up to eight different biological targets, effectively doubling the number of observable targets. The combination of synthetic probes with fluorescence lifetime multiplexing is thus a powerful approach for live-cell imaging.
Structure and Function of the β-Asp-Arg Polymerase Cyanophycin Synthetase 2
Sharon, Itai; Grogg, Marcel; Hilvert, Donald; et al. (2022)
ACS Chemical Biology
Cyanophycin is a biopolymer composed of long chains of β-Asp-Arg. It is widespread in nature, being synthesized by many clades of bacteria, which use it as a cellular reservoir of nitrogen, carbon, and energy. Two enzymes are known to produce cyanophycin: cyanophycin synthetase 1 (CphA1), which builds cyanophycin from the amino acids Asp and Arg by alternating between two separate reactions for backbone extension and side chain modification, and cyanophycin synthetase 2 (CphA2), which polymerizes β-Asp-Arg dipeptides. CphA2 is evolutionarily related to CphA1, but questions about CphA2's altered structure and function remain unresolved. Cyanophycin and related molecules have drawn interest as green biopolymers. Because it only has a single active site, CphA2 could be more useful than CphA1 for biotechnological applications seeking to produce modified cyanophycin. In this study, we report biochemical assays on nine cyanobacterial CphA2 enzymes and report the crystal structure of CphA2 from Gloeothece citriformis at 3.0 Å resolution. The structure reveals a homodimeric, three-domain architecture. One domain harbors the polymerization active site and the two other domains have structural roles. The structure and biochemical assays explain how CphA2 binds and polymerizes β-Asp-Arg and highlights differences in in vitro oligomerization and activity between CphA2 enzymes. Using the structure and distinct activity profile as a guide, we introduced a single point mutation that converted Gloeothece citriformis CphA2 from a primer-dependent enzyme into a primer-independent enzyme.
Exhaustive proteome mining for functional MHC-I ligands
Koch, Christian P.; Perna, Anna M.; Weissmüller, Sabrina; et al. (2013)
ACS Chemical Biology
A Small-Molecule Inhibitor of Lin28
Roos, Martina; Pradère, Ugo; Ngondo, Richard P.; et al. (2016)
ACS Chemical Biology
Launching Spiking Ligands into a Protein Protein Interface: A Promising Strategy To Destabilize and Break Interface Formation in a tRNA Modifying Enzyme
Immekus, Florian; Barandun, Luzi Jakob; Betz, Michael; et al. (2013)
ACS Chemical Biology
Selection of Carbonic Anhydrase IX Inhibitors from One Million DNA-Encoded Compounds
Buller, Fabian; Steiner, Martina; Frey, Katharina; et al. (2011)
ACS Chemical Biology
Critical evaluation and rate constants of chemoselective ligation reactions for stoichiometric conjugations in water
Saito, Fumito; Noda, Hidetoshi; Bode, Jeffrey W. (2015)
ACS Chemical Biology

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Journal: ACS Chemical Biology

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