Analytical Biochemistry

Journal: Analytical Biochemistry

Abbreviation

Anal Biochem

Publisher

Elsevier

ISSN

0003-2697
1096-0309

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Publications 1 - 10 of 23

Quantitative metabolome analysis using liquid chromatography-high-resolution mass spectrometry
Kiefer, Patrick; Portais, Jean-Charles; Vorholt, Julia A. (2008)
Analytical Biochemistry
Spectrophotometric quantification of horseradish peroxidase with o-phenylenediamine
Fornera, Sara; Walde, Peter (2010)
Analytical Biochemistry
Rapid estimation of the energy charge from cell lysates using matrix-assisted laser desorption/ionization mass spectrometry
Steinhoff, Robert F.; Krismer, Jasmin; Eyer, Klaus; et al. (2014)
Analytical Biochemistry
Interaction kinetics of salmeterol with egg phosphatidylcholine liposomes by surface plasmon resonance
Lombardi, Dario; Cuenoud, Bernard; Wunderli-Allenspach, Heidi; et al. (2009)
Analytical Biochemistry
Role of arginine in chemical cross-linking with N-hydroxysuccinimide esters
Mädler, Stefanie; Gschwind, Sabrina; Zenobi, Renato (2010)
Analytical Biochemistry
Detection of protein complex interactions via a Blue Native-PAGE retardation assay
Swamy, Mahima; Molnar, Eszter; Bock, Thomas; et al. (2009)
Analytical Biochemistry
A continuous fluorescent method for measuring Na+ transport
von Ballmoos, Christoph; Dimroth, Peter (2004)
Analytical Biochemistry
Screening for potential interaction partners with surface plasmon resonance imaging coupled to MALDI mass spectrometry
Anders, Ulrike; Gulotti-Georgieva, Maya; Zelger-Paulus, Susann; et al. (2021)
Analytical Biochemistry
We coupled SPR imaging (SPRi) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) to identify new potential RNA binders. Here, we improve this powerful method, especially by optimizing the proteolytic digestion (type of reducing agent, its concentration, and incubation time), to work with complex mixtures, specifically a lysate of the rough mitochondrial fraction from yeast. The advantages of this hyphenated method compared to column-based or separate analyses are (i) rapid and direct visual readout from the SPRi array, (ii) possibility of high-throughput analysis of different interactions in parallel, (iii) high sensitivity, and (iv) no sample loss or contamination due to elution or micro-recovery procedures. The model system used is a catalytically active RNA (group IIB intron from Saccharomyces cerevisiae, Sc.ai5γ) and its cofactor Mss116. The protein supports the RNA folding process and thereby the subsequent excision of the intronic RNA from the coding part. Using the novel approach of coupling SPR with MALDI MS, we report the identification of potential RNA-binding proteins from a crude yeast mitochondrial lysate in a non-targeted approach. Our results show that proteins other than the well-known cofactor Mss116 interact with Sc.ai5γ (Dbp8, Prp8, Mrp13, and Cullin-3), suggesting that the intron folding and splicing are regulated by more than one cofactor in vivo.
Analysis of polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis alongside in vitro-generated reference peptides
Lemberg, Marius K.; Martoglio, Bruno (2003)
Analytical Biochemistry
Determination of active enzyme concentration using activity-based probes and direct mass spectrometric readout
Bovet, Cédric; Zenobi, Renato (2008)
Analytical Biochemistry

Publications 1 - 10 of 23

Journal: Analytical Biochemistry

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