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Journal: Advanced Biology

Abbreviation

Adv. Biology

Publisher

Wiley-VCH

Journal Volumes

ISSN

2701-0198

Description

Filters

2021 - 20234
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Publications 1 - 4 of 4
  • Synthetic Biological Approaches for Optogenetics and Tools for Transcriptional Light‐Control in Bacteria
    Item type: Review Article
    Baumschlager, Armin; Khammash, Mustafa Hani (2021)
    Advanced Biology
  • Microfluidic Co-Culture Platform to Recapitulate the Maternal–Placental–Embryonic Axis
    Item type: Journal Article
    Boos, Julia A.; Misun, Patrick; Brunoldi, Giulia; et al. (2021)
    Advanced Biology
    Safety assessment of the effects of developmental toxicants on pregnant women is challenging, and systemic effects in embryo–maternal interactions are largely unknown. However, most developmental toxicity studies rely on animal trials, while in vitro platforms that recapitulate the maternal–placental–embryonic axis are missing. Here, the development of a dedicated microfluidic device for co-cultivation of a placental barrier and 3D embryoid bodies to enable systemic toxicity testing at the embryo–maternal interface is reported. The microfluidic platform features simple handling and recuperation of both tissue models, which facilitates post-hoc in-depth analysis at the tissue and single-cell level. Gravity-driven flow enables inter-tissue communication through the liquid phase as well as simple and robust operation and renders the platform parallelizable. As a proof of concept and to demonstrate platform use for systemic embryotoxicity testing in vitro, maternal exposure to plastic microparticles is emulated, and microparticle effects on the embryo–placental co-culture are investigated.
  • Metal–Organic Framework Mediated Radio-Enhancement Assessed in High-Throughput-Compatible 3D Tumor Spheroid Co-Cultures
    Item type: Journal Article
    Neuer, Anna Lena; Vogel, Alexandra; Gogos, Alexander; et al. (2023)
    Advanced Biology
    Inorganic nanomaterials have gained increasing attention in radiation oncology, owing to their radiation therapy enhancing properties. To accelerate candidate material selection and overcome the disconnect between conventional 2D cell culture and in vivo findings, screening platforms unifying high-throughput with physiologically relevant endpoint analysis based on 3D in vitro models are promising. Here, a 3D tumor spheroid co-culture model based on cancerous and healthy human cells is presented for the concurrent assessment of radio-enhancement efficacy, toxicity, and intratissural biodistribution with full ultrastructural context of radioenhancer candidate materials. Its potential for rapid candidate materials screening is showcased based on the example of nano-sized metal–organic frameworks (nMOFs) and direct benchmarking against gold nanoparticles (the current “gold standard”). Dose enhancement factors (DEFs) ranging between 1.4 and 1.8 are measured for Hf-, Ti-, TiZr-, and Au-based materials in 3D tissues and are overall lower than in 2D cell cultures, where DEF values exceeding 2 are found. In summary, the presented co-cultured tumor spheroid—healthy fibroblast model with tissue-like characteristics may serve as high-throughput platform enabling rapid, cell line-specific endpoint analysis for therapeutic efficacy and toxicity assessment, as well as accelerated radio-enhancer candidate screening.
  • Single-Cell Profiling Reveals Functional Heterogeneity and Serial Killing in Human Peripheral and Ex Vivo-Generated CD34+Progenitor-Derived Natural Killer Cells
    Item type: Journal Article
    Subedi, Nikita; Verhagen, Liesbeth Petronella; de Jonge, Paul; et al. (2023)
    Advanced Biology
    Increasing evidence suggests that natural killer (NK) cells are composed of distinct functional subsets. This multifunctional role has made them an attractive choice for anticancer immunotherapy. A functional NK cell repertoire is generated through cellular education, resulting in a heterogeneous NK cell population with distinct capabilities responding to different stimuli. The application of a high-throughput droplet-based microfluidic platform allows monitoring of NK cell-target cell interactions at the single-cell level and in real-time. A variable response of single NK cells toward different target cells is observed, and a distinct population of NK cells (serial killers) capable of inducing multiple target lysis is identified. By assessing the cytotoxic dynamics, it is shown that single umbilical cord blood-derived CD34+ hematopoietic progenitor (HPC)-NK cells display superior antitumor cytotoxicity. With an integrated analysis of cytotoxicity and cytokine secretion, it is shown that target cell interactions augment cytotoxic as well as secretory behavior of NK cells. By providing an integrated assessment of NK cell functions by microfluidics, this study paves the way to further functionally characterize NK cells ultimately aimed to improve cancer immunotherapy.
Publications 1 - 4 of 4