Journal: Applied Microbiology and Biotechnology

Abbreviation

Appl. microbiol. biotechnol.

Publisher

Springer

Journal Volumes

ISSN

0175-7598

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Publications 1 - 10 of 46
  • Single-gene knockout of a novel regulatory element confers ethionine resistance and elevates methionine production in Corynebacterium glutamicum
    Item type: Journal Article
    Mampel, Jörg; Schröder, Hartwig; Haefner, Stefan; et al. (2005)
    Applied Microbiology and Biotechnology
  • Methylotrophy in the thermophilic Bacillus methanolicus, basic insights and application for commodity production from methanol
    Item type: Journal Article
    Müller, Jonas E.N.; Heggeset, Tonje M.B.; Wendisch, Volker F.; et al. (2015)
    Applied Microbiology and Biotechnology
  • Selection and characterization of a SpaCBA pilus-secreting food-grade derivative of Lacticaseibacillus rhamnosus GG
    Item type: Journal Article
    Tytgat, Hanne L.P.; Rasinkangas, Pia; Ritari, Jarmo; et al. (2021)
    Applied Microbiology and Biotechnology
    Many studies have established the functional properties of Lacticaseibacillus rhamnosus GG, previously known as Lactobacillus rhamnosus GG, marketed worldwide as a probiotic. The extraordinary capacity of L. rhamnosus GG to bind to human mucus and influence the immune system especially stand out. Earlier, we have shown the key role of its SpaCBA sortase-dependent pili encoded by the spaCBA-srtC1 gene cluster herein. These heterotrimeric pili consist of a shaft pilin SpaA, a basal pilin SpaB, and tip pilin SpaC that contains a mucus-binding domain. Here, we set out to characterize a food-grade non-GMO mutant of L. rhamnosus GG, strain PA11, which secretes its pilins, rather than coupling them to the cell surface, due to a defect in the housekeeping sortase A. The sortase-negative strain PA11 was extensively characterized using functional genomics and biochemical approaches and found to secrete the SpaCBA pili into the supernatant. Given the functional importance and uniqueness of the mucus-binding pili of L. rhamnosus GG, strain PA11 offers novel opportunities towards the characterization and further therapeutic application of SpaCBA pili and their low-cost, large-scale production.
  • Discovery of novel fungal RiPP biosynthetic pathways and their application for the development of peptide therapeutics
    Item type: Review Article
    Vogt, Eva; Künzler, Markus (2019)
    Applied Microbiology and Biotechnology
    Bioactive peptide natural products are an important source of therapeutics. Prominent examples are the antibiotic penicillin and the immunosuppressant cyclosporine which are both produced by fungi and have revolutionized modern medicine. Peptide biosynthesis can occur either non-ribosomally via large enzymes referred to as non-ribosomal peptide synthetases (NRPS) or ribosomally. Ribosomal peptides are synthesized as part of a larger precursor peptide where they are posttranslationally modified and subsequently proteolytically released. Such peptide natural products are referred to as ribosomally synthesized and posttranslationally modified peptides (RiPPs). Their biosynthetic pathways have recently received a lot of attention, both from a basic and applied research point of view, due to the discoveries of several novel posttranslational modifications of the peptide backbone. Some of these modifications were so far only known from NRPSs and significantly increase the chemical space covered by this class of peptide natural products. Latter feature, in combination with the promiscuity of the modifying enzymes and the genetic encoding of the peptide sequence, makes RiPP biosynthetic pathways attractive for synthetic biology approaches to identify novel peptide therapeutics via screening of de novo generated peptide libraries and, thus, exploit bioactive peptide natural products beyond their direct use as therapeutics. This review focuses on the recent discovery and characterization of novel RiPP biosynthetic pathways in fungi and their possible application for the development of novel peptide therapeutics.
  • Backwash intensity and frequency impact the microbial community structure and function in a fixed-bed biofilm reactor
    Item type: Journal Article
    Li, Xu; Yuen, Wangki; Morgenroth, Eberhard; et al. (2012)
    Applied Microbiology and Biotechnology
  • Acetate scavenging activity in Escherichia coli
    Item type: Journal Article
    Renilla, Sergio; Bernal, Vicente; Fuhrer, Tobias; et al. (2012)
    Applied Microbiology and Biotechnology
  • Biotransformation of limonene by bacteria, fungi, yeasts, and plants
    Item type: Review Article
    Duetz, W.A.; Bouwmeester, H.; Beilen, J.B. van; et al. (2003)
    Applied Microbiology and Biotechnology
  • Production of 3-hydroxypropionaldehyde using a two-step process with Lactobacillus reuteri
    Item type: Journal Article
    Doleyres, Y.; Beck, P.; Vollenweider, S.; et al. (2005)
    Applied Microbiology and Biotechnology
  • How the interaction of Listeria monocytogenes and Acanthamoeba spp. affects growth and distribution of the food borne pathogen
    Item type: Journal Article
    Schuppler, Markus (2014)
    Applied Microbiology and Biotechnology
    Listeria monocytogenes is a foodborne opportunistic pathogen capable to switch from an environmental saprophyte to a potentially fatal human pathogen. The fact that the pathogen maintains the genes suitable for an elaborate infectious process indicates that these genes are required to survive in the environment. However, no environmental host reservoir for L. monocytogenes has been identified so far. The similarity of free-living, bacteria-scavenging amoebae to macrophages led to the hypothesis that protozoa may represent the missing link in the ecology and pathology of L. monocytogenes. Consequently, numerous studies have been published reporting on the potential of Acanthamoeba spp. to serve as host for a variety of pathogenic bacteria. However, the data on the interaction of L. monocytogenes with Acanthamoeba spp. are inconsistent and relatively little information on the impact of this interaction on growth and distribution of the foodborne pathogen is currently available. Hence, this review focuses on the interaction of L. monocytogenes and Acanthamoeba spp. affecting survival and growth of the foodborne pathogen in natural and man-made environments, in order to highlight the potential impact of this interplay on food safety and human health.
  • Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
    Item type: Journal Article
    Tao, Yile; Yue, Yang; Qiu, Guangyu; et al. (2022)
    Applied Microbiology and Biotechnology
    The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the next wave. Therefore, 14 primer sets recommended by the World Health Organization (WHO) was evaluated on testing both clinical patient and environmental samples with the gold standard diagnosis method, TaqMan-based RT-qPCR, and a cheaper alternative method, SYBR Green-based RT-qPCR. Using suitable primer sets, such as ORF1ab, 2019_nCoV_N1 and 2019_nCoV_N3, the performance of the SYBR Green approach was comparable or better than the TaqMan approach, even when considering the newly dominating or emerging variants, including Delta, Eta, Kappa, Lambda, Mu, and Omicron. ORF1ab and 2019_nCoV_N3 were the best combination for sensitive and reliable SARS-CoV-2 molecular diagnostics due to their high sensitivity, specificity, and broad accessibility.
Publications 1 - 10 of 46