Journal: Investigative Ophthalmology & Visual Science

Abbreviation

Invest Ophthalmol Vis Sci

Publisher

Association for Research in Vision and Ophthalmology

Journal Volumes

ISSN

0146-0404

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Publications 1 - 10 of 25
  • Syndromic choroideremia
    Item type: Journal Article
    Poloschek, Charlotte M.; Kloeckener-Gruissem, Barbara; Hansen, Lutz L.; et al. (2008)
    Investigative Ophthalmology & Visual Science
  • Identification and functional characterization of a novel rhodopsin mutation associated with autosomal dominant CSNB
    Item type: Journal Article
    Zeitz, Christina; Gross, Alecia K.; Leifert, Dorothee; et al. (2008)
    Investigative Ophthalmology & Visual Science
  • Axonal pathways and propagation speeds in the human retinal nerve fiber layer. Does speed compensate for length?
    Item type: Other Conference Item
    Franke, Felix; Büttner, Marc; Znidaric, Matej; et al. (2024)
    Investigative Ophthalmology & Visual Science
  • The Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal Disease
    Item type: Journal Article
    Schori, Christian; Trachsel, Christian; Grossmann, Jonas; et al. (2018)
    Investigative Ophthalmology & Visual Science
    Purpose: In contrast to neovascular AMD (nAMD), no treatment option exists for dry AMD. Hence, the identification of specific biomarkers is required to facilitate diagnosis and therapy of dry AMD. Methods: The proteome of 34 vitreous humor samples (dry AMD: n = 6; nAMD: n = 10; proliferative diabetic retinopathy [PDR]: n = 9; epiretinal membrane [ERM]: n = 9) was analyzed by liquid chromatography coupled mass spectrometry. Then, label-free relative quantification of dry AMD, nAMD, and PDR relative to ERM, which was defined as the reference group, was performed. Application of a bioinformatics pipeline further analyzed the vitreous proteome by cluster and gene set enrichment analysis. A selection of differentially regulated proteins was validated by ELISA. Results: A total of 677 proteins were identified in the vitreous of the four patient groups and quantified relatively to ERM. Different clusters of regulated proteins for each patient group were identified and showed characteristic enrichment of specific pathways including “oxidative stress” for dry AMD, “focal adhesion” for nAMD, and “complement and coagulation cascade” for PDR patients. We identified cholinesterase (CHLE) to be specifically upregulated in dry AMD and ribonuclease (pancreatic; RNAS1) together with serine carboxypeptidase (probable; CPVL) to be upregulated in both forms of AMD. Conclusions: The described pathways specific for the different patient groups and the identification of characteristic differentially regulated proteins provide a first step toward the definition of biomarkers for dry AMD. The presented data will facilitate the investigation of mechanistic connections of proteins to the respective disease.
  • Effect of Gabapentin/Memantine on the Infantile Nystagmus Syndrome in the Zebrafish Model: Implications for the Therapy of Ocular Motor Diseases
    Item type: Journal Article
    Bögli, Stefan Y.; Afthinos, Maresa; Huang, Melody Ying-Yu (2017)
    Investigative Ophthalmology & Visual Science
    Purpose: Infantile nystagmus syndrome (INS) is a disorder characterized by typical horizontal eye oscillations. Due to the uncertain etiology of INS, developing specific treatments remains difficult. Single reports demonstrated, on limited measures, alleviating effects of gabapentin and memantine. In the current study, we employed the zebrafish INS model belladonna (bel) to conduct an in-depth study of how gabapentin and memantine interventions alleviate INS signs, which may further restore visual conditions in affected subjects. Moreover, we described the influence of both medications on ocular motor functions in healthy zebrafish, evaluating possible iatrogenic effects. Methods: Ocular motor function and INS characteristics were assessed by eliciting optokinetic response, spontaneous nystagmus, and spontaneous saccades in light and in dark, in 5- to 6-day postfertilization bel larvae and heterozygous siblings. Single larvae were recorded before and after a 1-hour drug treatment (200 mM gabapentin/0.2 mM memantine). Results: Both interventions significantly reduced nystagmus intensity (gabapentin: 59.98%, memantine: 39.59%). However, while the application of gabapentin affected all tested ocular motor functions, memantine specifically reduced nystagmus amplitude and intensity, and thus left controls completely unaffected. Finally, both drug treatments resulted in specific changes in nystagmus waveform and velocity. Conclusions: Our study provides deeper insight into gabapentin and memantine treatment effect in the zebrafish INS model. Moreover, this study should establish zebrafish as a pharmacologic animal model for treating nystagmus and ocular motor disease, serving as a basis for future large-scale drug screenings.
  • The Anatomy of the Muscle Insertion (Scleromuscular Junction) of the Lateral and Medial Rectus Muscle in Humans
    Item type: Journal Article
    Jaggi, Gregor P.; Laeng, Hubert R.; Müntener, Markus; et al. (2005)
    Investigative Ophthalmology & Visual Science
  • Unravelling Stimulus Direction Dependency of Visual Acuity in Larval Zebrafish by Consistent Eye Displacements Upon Optokinetic Stimulation
    Item type: Journal Article
    Bögli, Stefan Y.; Afthinos, Maresa; Bertolini, Giovanni; et al. (2016)
    Investigative Ophthalmology & Visual Science
    Purpose: Impairment of visual acuity (VA) can be seen early on in various diseases and has a major impact on patients' daily activities. Zebrafish is an important model for studying visual disorders. We developed a new method in zebrafish larva to easily and precisely measure the VA, which should allow for better estimation of affected vision such as after genetic manipulation or pharmacologic intervention. Methods: We used an optokinetic reflex (OKR) paradigm with a staircase technique to estimate VA of zebrafish larva. Consistent eye displacements were used as the indicator for OKR. We measured VA and determined the dependence of VA on clockwise and counterclockwise horizontal stimulus directions. Results: Visual acuity in zebrafish larva was estimated to be 0.179 ± 0.013 cyc/deg binocularly and 0.129 ± 0.008 cyc/deg (left eye) and 0.128 ± 0.012 cyc/deg (right eye) monocularly. We found within single subjects spatial frequency thresholds that showed highly significant differences between the two horizontal stimulus directions. Average higher and lower binocular thresholds were 0.181 ± 0.026 and 0.158 ± 0.014 cyc/deg, respectively. Importantly, no correlations were found between spatial frequency thresholds and average median peak slow-phase eye velocities (SPV) of OKR in all experiments. Conclusions: Consistent eye displacements evoked by OKR stimuli can be used as an indirect measure of VA in zebrafish larva. Conversely, using SPV of OKR to determine VA does not seem to be accurate. With our method, single larva showed significantly different VA depending on stimulus directions, which might reflect asymmetric maturation of retinal and/or visual pathway structures.
  • The filling characteristics of indocyanine green and fluorescein dyes measured by simultaneous digital ICG and fluorescein angiography
    Item type: Other Conference Item
    Torok, B.; Eberhard, R.; Bischoff, P.; et al. (2003)
    Investigative Ophthalmology & Visual Science
  • Mobility Experiments With Microrobots for Minimally Invasive Intraocular Surgery
    Item type: Journal Article
    Ullrich, Franziska; Bergeles, Christos; Pokki, Juho; et al. (2013)
    Investigative Ophthalmology & Visual Science
  • New Mouse Retinal Stroke Model Reveals Direction-Selective Circuit Damage Linked to Permanent Optokinetic Response Loss
    Item type: Journal Article
    Joly, Sandrine; Guzik-Kornacka, Anna; Schwab, Martin E.; et al. (2014)
    Investigative Ophthalmology & Visual Science
Publications 1 - 10 of 25