Journal: Transgenic Research

Abbreviation

Transgenic Res

Publisher

Springer

Journal Volumes

ISSN

0962-8819
1573-9368

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Publications 1 - 8 of 8
  • The environment exerts a greater influence than the transgene on the transcriptome of field-grown wheat expressing the Pm3b allele
    Item type: Journal Article
    Diaz Quijano, Carolina; Brunner, Susanne; Keller, Beat; et al. (2015)
    Transgenic Research
    Wheat provides 20 % of the calories consumed worldwide. Powdery mildew infections of wheat can result in more than 30 % yield loss but it has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile study using GeneChip Wheat Genome Array and Fluidigm 96.96 Dynamic Arrays was performed to obtain insights into the mode of action of Pm3b and to elucidate the molecular basis of pleiotropic effects observed in three out of four independent transgenic events under field conditions. A cluster analysis of the microarray data and a principal component analysis of the Fluidigm 96.96 Dynamic Arrays data showed that transgenic lines and null segregants grouped together. The microarray analysis of samples from fungicide-treated plants revealed that significantly fewer genes were differentially expressed in Pm3b#1 than in Pm3b#2, which had a pleiotropic phenotype in the field, compared to their null segregants. Together, our data provide evidence that the environment influenced gene expression in the Pm3b lines more than the transgene itself.
  • Novel tools to discern the pluripotency gene network establishment
    Item type: Other Conference Item
    Chantzoura, E.; Skylaki, S.; Johnsson, A.; et al. (2014)
    Transgenic Research
  • RNAi mediated knockdown of gene activities in transgenic rats
    Item type: Other Conference Item
    Schönig, Kai; Tews, Björn; Berger, Stefan; et al. (2010)
    Transgenic Research
  • Comparison between volatile emissions from transgenic apples and from two representative classically bred apple cultivars
    Item type: Journal Article
    Vogler, Ute; Rott, Anja S.; Gessler, Cesare; et al. (2010)
    Transgenic Research
  • Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1
    Item type: Journal Article
    Dumeau, Charles-Etienne; Monfort, Asun; Kissling, Lucas; et al. (2019)
    Transgenic Research
    CRISPR-associated (Cas) nucleases are established tools for engineering of animal genomes. These programmable RNA-guided nucleases have been introduced into zygotes using expression vectors, mRNA, or directly as ribonucleoprotein (RNP) complexes by different delivery methods. Whereas microinjection techniques are well established, more recently developed electroporation methods simplify RNP delivery but can provide less consistent efficiency. Previously, we have designed Cas12a-crRNA pairs to introduce large genomic deletions in the Ubn1, Ubn2, and Rbm12 genes in mouse embryonic stem cells (ESC). Here, we have optimized the conditions for electroporation of the same Cas12a RNP pairs into mouse zygotes. Using our protocol, large genomic deletions can be generated efficiently by electroporation of zygotes with or without an intact zona pellucida. Electroporation of as few as ten zygotes is sufficient to obtain a gene deletion in mice suggesting potential applicability of this method for species with limited availability of zygotes.
  • TT2023 meeting report on the 18th Transgenic Technology meeting in Houston, United States
    Item type: Journal Article
    Savić, Nataša; Ristić, Zorica; Selbert, Stefan (2024)
    Transgenic Research
    The 18th Transgenic Technology Meeting, held in Houston, Texas from November 12–15, 2023, was a vibrant international forum. It brought together nearly 400 delegates to discuss advances in transgenic technologies and the science these technologies support. Among them were 329 in-person and 70 remote delegates, representing 26 countries from 5 continents. The event, hosted by the International Society for Transgenic Technologies (ISTT), was set against the backdrop of the Hyatt Regency’s panoramic views, reflecting the innovative spirit of the conference. A notable precursor to the main conference was the Allele Design Pre-conference Workshop, which fostered in-depth discussions on state-of-the-art methodologies. The main conference encompassed ten sessions, delving into diverse topics from Precision Animal Models of Human Disease to the use of transgenic animals in Space Biology. Eighty posters provided for a lively exchange of ideas, while the ISTT Prize and other awards highlighted the event’s commitment to excellence. Beyond the conference halls, attendees had the opportunity to venture into Houston’s Museum District, home to 19 museums in the downtown area, or indulge in unique dining experiences.
  • Government regulation and public opposition create high additional costs for field trials with GM crops in Switzerland
    Item type: Journal Article
    Bernauer, Thomas; Tribaldos, Theresa; Luginbühl, Carolin; et al. (2011)
    Transgenic Research
  • Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses
    Item type: Journal Article
    Subhankar, Roy-Barman; Sautter, Christof; Chattoo, Bharat B. (2006)
    Transgenic Research
Publications 1 - 8 of 8