Biointerphases

Journal: Biointerphases

Publisher

AVS

ISSN

1559-4106
1934-8630

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Publications 1 - 10 of 10

On cell separation with topographically engineered surfaces
Franco, Davide; Klingauf, Mirko; Cecchini, Marco; et al. (2013)
Biointerphases
Background Topographical modifications of the surface influence several cell functions and can be exploited to modulate cellular activities such as adhesion, migration and proliferation. These complex interactions are cell-type specific, therefore engineered substrates featuring patterns of two or more different topographies may be used to obtain the selective separation of different cell lineages. This process has the potential to enhance the performance of biomedical devices promoting, for example, the local coverage with functional tissues while demoting the onset of inflammatory reactions. Findings & Conclusions Here we present a computational tool, based on Monte Carlo simulation, which decouples the contribution of cell proliferation and migration and predicts the cell-separation performance of topographically engineered substrates. Additionally, we propose an optimization procedure to shape the topographically engineered areas of a substrate and obtain maximal cell separation.
Poly(L-lysine)-grafted-poly(ethylene glycol)-based surface-chemical gradients
Morgenthaler, Sara; Zink, Christian; Staedler, Brigitte; et al. (2006)
Biointerphases
Phagocytosis of poly(L-lysine)-graft-poly(ethylene glycol) coated microspheres by antigen presenting cells
Wattendorf, U.; Koch, M.C.; Walter, E.; et al. (2006)
Biointerphases
Fluorescence-based in situ assay to probe the viability and growth kinetics of surface-adhering and suspended recombinant bacteria
Avalos Vizcarra, Ima; Emge, Philippe; Miermeister, Philipp; et al. (2013)
Biointerphases
Bacterial adhesion and biofilm growth can cause severe biomaterial-related infections and failure of medical implants. To assess the antifouling properties of engineered coatings, advanced approaches are needed for in situ monitoring of bacterial viability and growth kinetics as the bacteria colonize a surface. Here, we present an optimized protocol for optical real-time quantification of bacterial viability. To stain living bacteria, we replaced the commonly used fluorescent dye SYTO® 9 with endogenously expressed eGFP, as SYTO® 9 inhibited bacterial growth. With the addition of nontoxic concentrations of propidium iodide (PI) to the culture medium, the fraction of live and dead bacteria could be continuously monitored by fluorescence microscopy as demonstrated here using GFP expressing Escherichia coli as model organism. The viability of bacteria was thereby monitored on untreated and bioactive dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAC)-coated glass substrates over several hours. Pre-adsorption of the antimicrobial surfaces with serum proteins, which mimics typical protein adsorption to biomaterial surfaces upon contact with host body fluids, completely blocked the antimicrobial activity of the DMOAC surfaces as we observed the recovery of bacterial growth. Hence, this optimized eGFP/PI viability assay provides a protocol for unperturbed in situ monitoring of bacterial viability and colonization on engineered biomaterial surfaces with single-bacteria sensitivity under physiologically relevant conditions.
Creation of a functional heterogeneous vesicle array via DNA controlled surface sorting onto a spotted microarray
Städler, Brigitte; Bally, Marta; Grieshaber, Dorothee; et al. (2006)
Biointerphases
Measuring cell adhesion forces during the cell cycle by force spectroscopy
Weder, Gilles; Vörös, Janos; Giazzon, Marta; et al. (2009)
Biointerphases
Surface plasmon optical detection of beta-lactamase binding to different interfacial matrices combined with fiber optic absorbance spectroscopy for enzymatic activity assays
Xu, Fei; Zhen, Guoliang; Textor, Marcus; et al. (2006)
Biointerphases
Characterization of supported lipid bilayers incorporating the phosphoinositides phosphatidylinositol 4,5-biphosphate and phosphoinositol-3,4,5-triphosphate by complementary techniques
Baumann, Martina K.; Amstad, Esther; Mashagi, Alireza; et al. (2010)
Biointerphases
Monitoring kinetics of surface initiated atom transfer radical polymerization by quartz crystal microbalance with dissipation
Ma, Hongwei; Textor, Marcus; Clark, Robert L.; et al. (2006)
Biointerphases
Microfabricated three-dimensional environments for single cell studies
Dusseiller, Marc R.; Smith, Michael L.; Vogel, Viola; et al. (2006)
Biointerphases

Publications 1 - 10 of 10

Journal: Biointerphases

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