Glycobiology

Journal: Glycobiology

Publisher

Oxford University Press

ISSN

0959-6658

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Publications 1 - 10 of 51

Dimerization of the fungal defense lectin CCL2 is essential for its toxicity against nematodes
Bleuler-Martinez, Silvia; Stutz, Katrin; Sieber, Ramon; et al. (2017)
Glycobiology
Structure-function relationship of a novel fucoside-binding fruiting body lectin from Coprinopsis cinerea exhibiting nematotoxic activity
Bleuler-Martinez, Silvia; Varrot, Annabelle; Olieric, Vincent; et al. (2022)
Glycobiology
Lectins are non-immunoglobulin-type proteins that bind to specific carbohydrate epitopes and play important roles in intra- and inter-organismic interactions. Here, we describe a novel fucose-specific lectin, termed CML1, which we identified from fruiting body extracts of Coprinopsis cinerea. For further characterization, the coding sequence for CML1 was cloned and heterologously expressed in Escherichia coli. Feeding of CML1-producing bacteria inhibited larval development of the bacterivorous nematode Caenorhabditis tropicalis, but not of C. elegans. The crystal structure of the recombinant protein in its apo-form and in complex with H type I or Lewis A blood group antigens was determined by X-ray crystallography. The protein folds as a sandwich of 2 antiparallel β-sheets and forms hexamers resulting from a trimer of dimers. The hexameric arrangement was confirmed by small-angle X-ray scattering (SAXS). One carbohydrate-binding site per protomer was found at the dimer interface with both protomers contributing to ligand binding, resulting in a hexavalent lectin. In terms of lectin activity of recombinant CML1, substitution of the carbohydrate-interacting residues His54, Asn55, Trp94, and Arg114 by Ala abolished carbohydrate-binding and nematotoxicity. Although no similarities to any characterized lectin were found, sequence alignments identified many non-characterized agaricomycete proteins. These results suggest that CML1 is the founding member of a novel family of fucoside-binding lectins involved in the defense of agaricomycete fruiting bodies against predation by fungivorous nematodes.
P-selectin mediates metastatic progression through binding to sulfatides on tumor cells
Garcia, Josep; Callewaert, Nico; Borsig, Lubor (2007)
Glycobiology
Double Mutants as Tools for Elucidating N-glycosylation Pathways in Caenorhabditis elegans
Yan, Shi; Paschinger, Katharina; Bleuler-Martinez, Silvia; et al. (2011)
Glycobiology
Expression and characterization of a human cDNA that complements the temperature-sensitive defect in dolichol kinase activity in the yeast sec59-1 mutant: the enzymatic phosphorylation of dolichol and diacylglycerol are catalyzed by separate CTP-mediated kinase activities in Saccharomyces cerevisiae
Fernandez, Fabiana; Shridas, Preetha; Jiang, Songmin; et al. (2002)
Glycobiology
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis
Frank, Christian G.; Aebi, Markus (2005)
Glycobiology
The N-X-S/T consensus sequence is required but not sufficient for bacterial N-linked protein glycosylation
Nita-Lazar, M.; Wacker, M.; Schegg, B.; et al. (2005)
Glycobiology
Characterization of a 21 amino acid peptide sequence of the laminin G2 domain that is involved in HNK-1 carbohydrate binding and cell adhesion
Hall, Heike; Vorherr, Thomas; Schachner, Melitta (1995)
Glycobiology
Dynamic tracing of sugar metabolism reveals the mechanisms of action of synthetic sugar analogs
van Scherpenzeel, Monique; Conte, Federica; Büll, Christian; et al. (2022)
Glycobiology
Synthetic sugar analogs are widely applied in metabolic oligosaccharide engineering (MOE) and as novel drugs to interfere with glycoconjugate biosynthesis. However, mechanistic insights on their exact cellular metabolism over time are mostly lacking. We combined ion-pair UHPLC-QqQ mass spectrometry using tributyl- and triethylamine buffers for sensitive analysis of sugar metabolites in cells and organisms and identified low abundant nucleotide sugars, such as UDP-arabinose in human cell lines and CMP-sialic acid (CMP-NeuNAc) in Drosophila. Furthermore, MOE revealed that propargyloxycarbonyl (Poc) labeled ManNPoc was metabolized to both CMP-NeuNPoc and UDP-GlcNPoc. Finally, time-course analysis of the effect of antitumor compound 3Fax-NeuNAc by incubation of B16-F10 melanoma cells with N-acetyl-D-[UL-13C6]glucosamine revealed full depletion of endogenous ManNAc 6-phosphate and CMP-NeuNAc within 24 hour. Thus, dynamic tracing of sugar metabolic pathways provides a general approach to reveal time-dependent insights into the metabolism of synthetic sugars, which is important for the rational design of analogs with optimized effects.
Structural characterization of the N-linked pentasaccharide decorating glycoproteins of the halophilic archaeon Haloferax volcanii
Kandiba, Lina; Lin, Chia-wei; Aebi, Markus; et al. (2016)
Glycobiology

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Journal: Glycobiology

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