Current Opinion in Biotechnology

Journal: Current Opinion in Biotechnology

Abbreviation

Curr. opin. biotechnol.

Publisher

Elsevier

ISSN

0958-1669
1879-0429

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Publications 1 - 10 of 95

Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins
Dunne, Matthew; Prokhorov, Nikolai S.; Loessner, Martin J.; et al. (2021)
Current Opinion in Biotechnology
Bacteriophages (phages) use specialized tail machinery to deliver proteins and genetic material into a bacterial cell during infection. Attached at the distal ends of their tails are receptor binding proteins (RBPs) that recognize specific molecules exposed on host bacteria surfaces. Since the therapeutic capacity of naturally occurring phages is often limited by narrow host ranges, there is significant interest in expanding their host range via directed evolution or structure-guided engineering of their RBPs. Here, we describe the design principles of different RBP engineering platforms and draw attention to the mechanisms linking RBP binding and the correct spatial and temporal attachment of the phage to the bacterial surface. A deeper understanding of these mechanisms will directly benefit future engineering of more effective phage-based therapeutics. © 2021 Elsevier
The engineering of spatially linked microbial consortia – potential and perspectives
Ben Said, Sami; Tecon, Robin; Borer, Benedict; et al. (2020)
Current Opinion in Biotechnology
Traditional biotechnological applications of microorganisms employ mono-cultivation or co-cultivation in well-mixed vessels disregarding the potential of spatially organized cultures. Metabolic specialization and guided species interactions facilitated through spatial isolation would enable consortia of microbes to accomplish more complex functions than currently possible, for bioproduction as well as biodegradation processes. Here, we review concepts of spatially linked microbial consortia in which spatial arrangement is optimized to increase control and facilitate new species combinations. We highlight that genome-scale metabolic network models can inform the design and tuning of synthetic microbial consortia and suggest that a standardized assembly of such systems allows the combination of ‘incompatibles’, potentially leading to countless novel applications.
Advancing metabolic models with kinetic information
Link, Hannes; Christodoulou, Dimitris; Sauer, Uwe (2014)
Current Opinion in Biotechnology
Expanding the alkane oxygenase toolbox
Beilen, Jan B. van; Funhoff, Enrico G. (2005)
Current Opinion in Biotechnology
Combinatorial pathway optimization for streamlined metabolic engineering
Jeschek, Markus; Gerngross, Daniel; Panke, Sven (2017)
Current Opinion in Biotechnology
Elimination of metabolic flux imbalances in microbial cell factories is an important part in the establishment of viable biotechnological production processes. However, due to the high complexity of cellular metabolism, the limited a priori knowledge about the majority of production pathways and a lack of forward design standards, metabolic engineers strongly rely on empirical screening methodologies to achieve the required improvement of cell behavior. Combinatorial pathway engineering provides an interesting tool to identify global solutions for intricate pathways, but methods for the reduction of combinatorial library size are inevitably required to restrict the experimental effort to an affordable size. Here we review recent advances from this field by scrutinizing commonly applied diversification methods and highlighting crucial strategies for the minimization of experimental effort.
Computational design tools for synthetic biology
Marchisio, Mario A.; Stelling, Jörg (2009)
Current Opinion in Biotechnology
At the crossroads of biology and electronics
Unal, Gokberk; Fussenegger, Martin (2025)
Current Opinion in Biotechnology
All cells are innately equipped with systems to detect and respond to electrical inputs in the form of reactive oxygen species, redox signaling, or membrane depolarization through ion exchange. Electrogenetics aims to leverage these cellular systems to create interfaces between biology and electronics, in order to achieve levels of precision in spatiotemporal control of gene and protein expression that are not possible with chemo-, opto-, or thermogenetics. In this review, we discuss the impact, challenges, and prospects of electrogenetics in the context of recent cutting-edge applications.
Design principles for adaptive and evolving engineered living materials
Cui , Yifan; Tibbitt, Mark W.; Lu , Timothy K.; et al. (2026)
Current Opinion in Biotechnology
Engineered living materials (ELMs) combine living cells, typically microorganisms, such as bacteria, yeasts, or filamentous fungi, with structural carrier matrices to form systems capable of sensing, growth, and self-repair. Most current designs emphasize programming the microbes to render otherwise static materials functional. A less explored dimension is leveraging reciprocal microbial–material interactions themselves to engineer adaptive and evolving living materials as integrated systems. Achieving such dynamic behavior requires understanding how support matrices influence microbial behavior and how cells, in turn, reshape material properties over time. This review outlines key modes of cell–material interactions as a framework for expanding the functional toolbox of ELMs and for creating sustainable and programmable materials that respond to their environments and evolve.
Challenges in synthetically designing mammalian circadian clocks
Susaki, Etsuo A.; Stelling, Jörg; Ueda, Hiroki R. (2010)
Current Opinion in Biotechnology
Strong cation exchange (SCX) based analytical methods for the targeted analysis of protein post-translational modifications
Mohammed, Shabaz; Heck, Albert J.R. (2011)
Current Opinion in Biotechnology

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Journal: Current Opinion in Biotechnology

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