Journal: Genesis

Abbreviation

Genesis (N.Y.N.Y., 2000, Print)

Publisher

Wiley-Blackwell

Journal Volumes

ISSN

1526-954X
1526-968X

Description

Filters

2005 - 200912010 - 20163
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Publications1 - 4 of 4
  • Inducible and neuron-specific gene expression in the adult mouse brain with the rtTA2S-M2 system
    Item type: Journal Article
    Michalon, Aubin; Koshibu, Kyoko; Baumgärtel, Karsten; et al. (2005)
    Genesis
    To achieve inducible and reversible gene expression in the adult mouse brain, we exploited an improved version of the tetracycline‐controlled transactivator‐based system (rtTA2S‐M2, rtTA2 hereafter) and combined it with the forebrain‐specific CaMKIIα promoter. Several independent lines of transgenic mice carrying the CaMKIIα promoter‐rtTA2 gene were generated and examined for anatomical profile, doxycycline (dox)‐dependence, time course, and reversibility of gene expression using several lacZ reporter lines. In two independent rtTA2‐expressing lines, dox‐treatment in the diet induced lacZ reporter expression in neurons of several forebrain structures including cortex, striatum, hippocampus, amygdala, and olfactory bulb. Gene expression was dose‐dependent and was fully reversible. Further, a similar pattern of expression was obtained in three independent reporter lines, indicating the consistency of gene expression. Transgene expression could also be activated in the developing brain (P0) by dox‐treatment of gestating females. These new rtTA2‐expressing mice allowing inducible and reversible gene expression in the adult or developing forebrain represent useful models for future genetic studies of brain functions. genesis 43:205–212, 2005. © 2005 Wiley‐Liss, Inc.
  • Hoxb8-Cre mice
    Item type: Journal Article
    Witschi, Robert; Johansson, Torbjoern; Morscher, Giannina; et al. (2010)
    Genesis ~ Genesis
    The spinal cord is the first site of temporal and spatial integration of nociceptive signals in the pain pathway. Neuroplastic changes occurring at this site contribute critically to various chronic pain syndromes. Gene targeting in mice has generated important insights into these processes. However, the analysis of constitutive (global) gene‐deficient mice is often hampered by confounding effects arising from supraspinal sites. Here, we describe a novel Cre mouse line that expresses the Cre recombinase under the transcriptional control of the Hoxb8 gene. Within the neural axis of these mice, Hoxb8‐Cre expression is found in spinal cord neurons and glial cells, and in virtually all neurons of the dorsal root ganglia, but spares the brain apart from a few cells in the spinal trigeminal nucleus. The Hoxb8‐Cre mouse line should be a valuable new tool for the in vivo analysis of peripheral and spinal gene functions in pain pathways. genesis 48:596–602, 2010. © 2010 Wiley‐Liss, Inc.
  • Building a zoo of mice for genetic analyses
    Item type: Journal Article
    Johansson, Torbjörn; Broll, Ilja; Frenz, Theresa; et al. (2010)
    Genesis
  • Analysis of novel alleles of brother of tout-velu, the drosophila ortholog of human EXTL3 using a newly developed FRT42D ovoD chromosome
    Item type: Journal Article
    Lujan, Ernesto; Bornemann, Douglas J.; Rottig, Carmen; et al. (2016)
    Genesis
Publications1 - 4 of 4