Chromosome Research

Journal: Chromosome Research

Abbreviation

Chomosom. Res.

Publisher

Springer

ISSN

0967-3849
1573-6849

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Publications 1 - 10 of 12

Assays for mitotic chromosome condensation in live yeast and mammalian cells
Neurohr, Gabriel; Gerlich, Daniel W. (2009)
Chromosome Research
Cdk8 and Hira mutations trigger X chromosome elimination in naive female hybrid mouse embryonic stem cells
Halter, Kevin; Chen, Jingyi; Priklopil, Tadeas; et al. (2024)
Chromosome Research
Mouse embryonic stem cells (ESCs) possess a pluripotent developmental potential and a stable karyotype. An exception is the frequent loss of one X chromosome in female ESCs derived from inbred mice. In contrast, female ESCs from crosses between different Mus musculus subspecies often maintain two X chromosomes and can model X chromosome inactivation. Here we report that combined mutations of Hira and Cdk8 induce rapid loss of one X chromosome in a Mus musculus castaneus hybrid female ESC line that originally maintains two X chromosomes. We show that MEK1 inhibition, which is used for culturing naive pluripotent ESCs is sufficient to induce X chromosome loss. In conventional ESC media, Hira and Cdk8 mutant ESCs maintain both X chromosomes. Induction of X chromosome loss by switching to naive culture media allows us to perform kinetic measurements for calculating the chromosome loss rate. Our analysis shows that X chromosome loss is not explained by selection of XO cells, but likely driven by a process of chromosome elimination. We show that elimination of the X chromosome occurs with a rate of 0.3% per cell per division, which exceeds reported autosomal loss rates by 3 orders of magnitude. We show that chromosomes 8 and 11 are stably maintained. Notably, Xist expression from one of the two X chromosomes rescues X chromosomal instability in ΔHiraΔCdk8 ESCs. Our study defines mutations of Hira and Cdk8 as molecular drivers for X chromosome elimination in naive female ESCs and describes a cell system for elucidating the underlying mechanism.
Chromatin structure of ribosomal genes in Chironomus thummi (Diptera: Chironomidae)
Sanz, Cristina; Gorab, Eduardo; Ruiz, Maria Fernanda; et al. (2007)
Chromosome Research
Survey of repetitive sequences in Silene latifolia with respect to their distribution on sex chromosomes
Cermak, Tomas; Kubat, Zdenek; Hobza, Roman; et al. (2008)
Chromosome Research
Unbiased about chromosome segregation
Maiato, Helder; Barral, Yves (2013)
Chromosome Research
Expression of XIST sense and antisense in bovine fetal organs and cell cultures
Farazmand, Ali; Basrur, Parvathi K.; Stranzinger, Gerald; et al. (2004)
Chromosome Research
Spermatocyte apoptosis is not related with the number of chromosome B univalents in pachytene cells of the red fox (Vulpes vulpes) and Chinese raccoon dog (Nyctereutes procyonoides procyonoides)
Sosnowski, J.; Lukaszewicz, A.; Migalska, L.; et al. (2010)
Chromosome Research
Application of in situ fluorescent nick-translation to detection DNA methylation in B chromosomes of red fox (Vulpes vulpes) and Chinese raccoon dog (Nyctereutes procyonoides procyonoides)
Slota, E.; Wnuk, M.; Bugno, M.; et al. (2008)
Chromosome Research
Polyploidy in the Arabidopsis genus
Bomblies, Kirsten; Madlung, Andreas (2014)
Chromosome Research
Whole genome duplication (WGD), which gives rise to polyploids, is a unique type of mutation that duplicates all the genetic material in a genome. WGD provides an evolutionary opportunity by generating abundant genetic “raw material,” and has been implicated in diversification, speciation, adaptive radiation, and invasiveness, and has also played an important role in crop breeding. However, WGD at least initially challenges basic biological functions by increasing cell size, altering relationships between cell volume and DNA content, and doubling the number of homologous chromosome copies that must be sorted during cell division. Newly polyploid lineages often have extensive changes in gene regulation, genome structure, and may suffer meiotic or mitotic chromosome mis-segregation. The abundance of species that persist in nature as polyploids shows that these problems are surmountable and/or that advantages of WGD might outweigh drawbacks. The molecularly especially tractable Arabidopsis genus has several ancient polyploidy events in its history and contains several independent more recent polyploids. This genus can thus provide important insights into molecular aspects of polyploid formation, establishment, and genome evolution. The ability to integrate ecological and evolutionary questions with molecular and genetic understanding makes comparative analyses in this genus particularly attractive and holds promise for advancing our general understanding of polyploid biology. Here, we highlight some of the findings from Arabidopsis that have given us insights into the origin and evolution of polyploids.
The nuclear organization of Polycomb/Trithorax group response elements in larval tissues of Drosophila melanogaster
Fedorova, Elena; Sadoni, Nicolas; Dahlsveen, Ina K.; et al. (2008)
Chromosome Research

Publications 1 - 10 of 12

Journal: Chromosome Research

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