Journal: Liver International

Abbreviation

Publisher

Wiley-Blackwell

Journal Volumes

ISSN

1478-3223
0106-9543
1478-3231
1600-0676

Description

Filters

2015 - 201912020 - 20251
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Publications1 - 2 of 2
  • Reduced hepatic lipid content in Pten-haplodeficient mice because of enhanced AKT2/PKBβ Activation in skeletal muscle
    Item type: Journal Article
    Schultze, Simon M.; Dietrich, Maren; Hynx, Debby; et al. (2015)
    Liver International
  • Analysis of Hyperexpanded T Cell Clones in SARS-CoV-2 Vaccine-Associated Liver Injury by Spatial Proteomics and Transcriptomics
    Item type: Journal Article
    Uzun, Sarp; Pant, Asmita; Bartoszek, Ewelina; et al. (2025)
    Liver International
    Background and Aims: SARS-CoV-2 vaccine-associated liver injury (SVALI) is a rare event and its pathophysiology remains unclear. Previous studies have found an oligoclonal CD8+ T cell infiltrate and SARS-CoV-2 spike antigen-specific T cells in the liver of patients with SVALI. Therefore, we aimed to characterise the immune infiltrate in a liver explant from a patient with severe SVALI. Methods: T cell receptor sequencing, a novel combined multiplex immunofluorescence (mIF)-RNA in situ hybridisation (RISH) approach, and single cell spatial transcriptomics with the Xenium in situ platform were used to identify, track and characterise specific T cell clones in this liver sample. Results: T cell repertoire analysis revealed hyperexpanded clones with CDR3 sequences similar to previously identified SARS-CoV-2 spike antigen-specific T cells. The hyperexpanded clones were localised throughout the whole liver, but the concentration was higher at the portal interface. Many hyperexpanded T cells expressed cytotoxic granzymes A, B and K, but also tissue-resident markers such as CXCR6, CD69 and KLRB1. Conclusions: Spatial proteomics and spatial transcriptomics techniques allowed the localisation and characterisation of hyperexpanded CD8+ T cell clones at single cell level. They exhibited cytotoxic and tissue-resident memory properties, suggesting their involvement in the pathogenesis of SVALI.
Publications1 - 2 of 2