Miriam Ries


Last Name

Ries

First Name

Miriam

ORCID

0000-0002-3917-322X

Organisational unit

03695 - Hoffmann, Volker / Hoffmann, Volker

Filters

2019 - 201922020 - 20232
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Publications 1 - 4 of 4
  • Differential effects of anaesthesia on the contractility of lymphatic vessels in vivo
    Item type: Journal Article
    Bachmann, Samia B.; Proulx, Steven T.; He, Yuliang; et al. (2019)
    The Journal of Physiology
  • Differential effects of anaesthesia on the contractility of lymphatic vessels in vivo: authors’ reply
    Item type: Other Journal Item
    Bachmann, Samia B.; Proulx, Steven T.; He, Yuliang; et al. (2020)
    The Journal of Physiology
  • Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain
    Item type: Journal Article
    Ma, Qiaoli; Ries, Miriam; Decker, Yann; et al. (2019)
    Acta Neuropathologica
    The relationships between cerebrospinal fluid (CSF) and brain interstitial fluid are still being elucidated. It has been proposed that CSF within the subarachnoid space will enter paravascular spaces along arteries to flush through the parenchyma of the brain. However, CSF also directly exits the subarachnoid space through the cribriform plate and other perineural routes to reach the lymphatic system. In this study, we aimed to elucidate the functional relationship between CSF efflux through lymphatics and the potential influx into the brain by assessment of the distribution of CSF-infused tracers in awake and anesthetized mice. Using near-infrared fluorescence imaging, we showed that tracers quickly exited the subarachnoid space by transport through the lymphatic system to the systemic circulation in awake mice, significantly limiting their spread to the paravascular spaces of the brain. Magnetic resonance imaging and fluorescence microscopy through the skull under anesthetized conditions indicated that tracers remained confined to paravascular spaces on the surface of the brain. Immediately after death, a substantial influx of tracers occurred along paravascular spaces extending into the brain parenchyma. We conclude that under normal conditions a rapid CSF turnover through lymphatics precludes significant bulk flow into the brain.
  • Open pathways for cerebrospinal fluid outflow at the cribriform plate along the olfactory nerves
    Item type: Journal Article
    Spera, Irene; Cousin, Nikola; Ries, Miriam; et al. (2023)
    eBioMedicine
    Background: Routes along the olfactory nerves crossing the cribriform plate that extend to lymphatic vessels within the nasal cavity have been identified as a critical cerebrospinal fluid (CSF) outflow pathway. However, it is still unclear how the efflux pathways along the nerves connect to lymphatic vessels or if any functional barriers are present at this site. The aim of this study was to anatomically define the connections between the subarachnoid space and the lymphatic system at the cribriform plate in mice. Methods: PEGylated fluorescent microbeads were infused into the CSF space in Prox1-GFP reporter mice and decalcification histology was utilized to investigate the anatomical connections between the subarachnoid space and the lymphatic vessels in the nasal submucosa. A fluorescently-labelled antibody marking vascular endothelium was injected into the cisterna magna to demonstrate the functionality of the lymphatic vessels in the olfactory region. Finally, we performed immunostaining to study the distribution of the arachnoid barrier at the cribriform plate region. Findings: We identified that there are open and direct connections from the subarachnoid space to lymphatic vessels enwrapping the olfactory nerves as they cross the cribriform plate towards the nasal submucosa. Furthermore, lymphatic vessels adjacent to the olfactory bulbs form a continuous network that is functionally connected to lym- phatics in the nasal submucosa. Immunostainings revealed a discontinuous distribution of the arachnoid barrier at the olfactory region of the mouse. Interpretation: Our data supports a direct bulk flow mechanism through the cribriform plate allowing CSF drainage into nasal submucosal lymphatics in mice.
Publications 1 - 4 of 4