Parth Chansoria

Last Name

Chansoria

First Name

Parth

ORCID

0000-0002-6107-6848

Organisational unit

09626 - Bar-Nur, Ori / Bar-Nur, Ori

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Publications 1 - 10 of 15

Untethered: using remote magnetic fields for regenerative medicine
Chansoria, Parth; Liu, Hao; Christiansen, Michael; et al. (2023)
Trends in Biotechnology
Magnetic fields are increasingly being used for the remote, noncontact manipulation of cells and biomaterials for a wide range of regenerative medical (RM) applications. They have been deployed for their direct effects on biological systems or in conjunction with magnetic materials or magnetically tagged cells for a targeted therapeutic effect. In this work, we highlight the recent trends on the broad use of magnetic fields for the homing of therapeutic cells and particles at targeted tissue sites, biomimetic tissue fabrication, and control of cell fate and proliferation. We also survey the design and control principles of magnetic manipulation systems, including their capabilities and limitations, which can guide future research into developing more effective magnetic field-based regenerative strategies.
Additive Manufacturing of Nanocellulose Aerogels with Structure-Oriented Thermal, Mechanical, and Biological Properties
Sivaraman, Deeptanshu; Nagel, Yannick; Siqueira, Gilberto; et al. (2024)
Advanced Science
Additive manufacturing (AM) is widely recognized as a versatile tool for achieving complex geometries and customized functionalities in designed materials. However, the challenge lies in selecting an appropriate AM method that simultaneously realizes desired microstructures and macroscopic geometrical designs in a single sample. This study presents a direct ink writing method for 3D printing intricate, high-fidelity macroscopic cellulose aerogel forms. The resulting aerogels exhibit tunable anisotropic mechanical and thermal characteristics by incorporating fibers of different length scales into the hydrogel inks. The alignment of nanofibers significantly enhances mechanical strength and thermal resistance, leading to higher thermal conductivities in the longitudinal direction (65 mW m⁻¹ K⁻¹) compared to the transverse direction (24 mW m⁻¹ K⁻¹). Moreover, the rehydration of printed cellulose aerogels for biomedical applications preserves their high surface area (≈ 300 m² g⁻¹) while significantly improving mechanical properties in the transverse direction. These printed cellulose aerogels demonstrate excellent cellular viability (>90% for NIH/3T3 fibroblasts) and exhibit robust antibacterial activity through in situ-grown silver nanoparticles.
Macroporous Aligned Hydrogel Microstrands for 3D Cell Guidance
Rizzo, Riccardo; Bonato, Angela; Chansoria, Parth; et al. (2022)
ACS Biomaterials Science & Engineering
Tissue engineering strongly relies on the use of hydrogels as highly hydrated 3D matrices to support the maturation of laden cells. However, because of the lack of microarchitecture and sufficient porosity, common hydrogel systems do not provide physical cell-instructive guidance cues and efficient transport of nutrients and oxygen to the inner part of the construct. A controlled, organized cellular alignment and resulting alignment of secreted ECM are hallmarks of muscle, tendons, and nerves and play an important role in determining their functional properties. Although several strategies to induce cellular alignment have been investigated in 2D systems, the generation of cell-instructive 3D hydrogels remains a challenge. Here, we report on the development of a simple and scalable method to efficiently generate highly macroporous constructs featuring aligned guidance cues. A precross-linked bulk hydrogel is pressed through a grid with variable opening sizes, thus deconstructing it into an array of aligned, high aspect ratio microgels (microstrands) with tunable diameter that are eventually stabilized by a second photoclick cross-linking step. This method has been investigated and optimized both in silico and in vitro, thereby leading to conditions with excellent viability and organized cellular alignment. Finally, as proof of concept, the method has been shown to direct aligned muscle tissue maturation. These findings demonstrate the 3D physical guidance potential of our system, which can be used for a variety of anisotropic tissues and applications.
An Implantable Biohybrid Neural Interface Toward Synaptic Deep Brain Stimulation
Sifringer, Léo; Fratzl, Alex; Clément, Blandine F.; et al. (2025)
Advanced Functional Materials
In patients with sensory nerve loss, such as those experiencing optic nerve damage that leads to vision loss, the thalamus no longer receives the corresponding sensory input. To restore functional sensory input, it is necessary to bypass the damaged circuits, which can be achieved by directly stimulating the appropriate sensory thalamic nuclei. However, available deep brain stimulation electrodes do not provide the resolution required for effective sensory restoration. Therefore, this work develops an implantable biohybrid neural interface aimed at innervating and synaptically stimulating deep brain targets. The interface combines a stretchable stimulation array with an aligned microfluidic axon guidance system seeded with neural spheroids to facilitate the development of a 3 mm long nerve-like structure. A bioresorbable hydrogel nerve conduit is used as a bridge between the tissue and the biohybrid implant. Stimulation of the spheroids within the biohybrid structure in vitro and use of high-density CMOS microelectrode arrays show faithful activity conduction across the device. Although functional in vivo innervation and synapse formation has not yet been achieved in this study, implantation of the biohybrid nerve onto the mouse cortex shows that neural spheroids grow axons in vivo and remain functionally active for more than 22 days post-implantation.
Volumetric printed biomimetic scaffolds support in vitro lactation of human milk-derived mammary epithelial cells
Hasenauer, Amelia; Bevc, Kajetana; McCabe, Maxwell C.; et al. (2025)
Science Advances
The human breast is remarkably plastic and remodels with each birth to produce milk optimally suited for the changing demands of the newborn. This dynamic nature of lactation makes it challenging to study under controlled conditions. Given the health benefits of human milk, models of secretory mammary tissue would offer opportunities to study factors that influence this important food source. First, 3D models of the mammary duct/alveoli (D/A) were designed inspired by shapes found in vivo. Photoresins based on mammary decellularized extracellular matrix (dECM) were optimized to match the mechanical properties of native breast tissue. Next, these D/A models were printed with a volumetric printer and seeded with human milk-derived mammary epithelial cells (MECs). MECs formed stable epithelial layers on the printed surfaces and secreted the beta-casein and milk fat globules. This model offers exciting avenues to explore hormonal, nutritional, and mechanobiological factors involved in lactation, thereby improving understanding of lactation for the benefit of infants and their mothers.
Multiscale Hybrid Fabrication: Volumetric Printing Meets Two-Photon Ablation
Rizzo, Riccardo; Rütsche, Dominic; Liu, Hao; et al. (2023)
Advanced Materials Technologies
Multiscale printing of 3D perfusable geometries holds great potential for a range of applications, from microfluidic systems to organ-on-a-chip. However, the generation of freeform designs spanning from centimeter to micrometer features represents an unmet challenge for a single fabrication method and thus may require the convergence of two or more modalities. Leveraging the great advances in light-based printing, herein a hybrid strategy is introduced to tackle this challenge. By combining volumetric printing (VP) and high-resolution two-photon ablation (2PA), the possibility to create multiscale models with features ranging from mesoscale (VP) to microscale (2PA) is demonstrated. To successfully combine these two methods, micrometer-size defects generated during the VP process due to optical modulation instability and self-focusing phenomena are first eliminated. By optical tuning the refractive index of the photoresin, defect-free VP that can then be combined with 2PA is demonstrated. To facilitate the 2PA process and meet VP requirements, a purely protein-based photoclick photoresin combining gelatin-norbornene and gelatin-thiol is introduced. Finally, the possibility to generate complex organotypic 3D vasculature-like constructs with features ranging from ≈400 µm of VP to ≈2 µm of 2PA is demonstrated. This hybrid strategy opens new possibilities for multiscale printing, with particular potential for microfluidics and organ/tissue-on-a-chip technologies.
Filamented Light (FLight) Biofabrication of Highly Aligned Tissue-Engineered Constructs
Liu, Hao; Chansoria, Parth; Delrot, Paul; et al. (2022)
Advanced Materials
Cell-laden hydrogels used in tissue engineering generally lack sufficient 3D topographical guidance for cells to mature into aligned tissues. A new strategy called filamented light (FLight) biofabrication rapidly creates hydrogels composed of unidirectional microfilament networks, with diameters on the length scale of single cells. Due to optical modulation instability, a light beam is divided optically into FLight beams. Local polymerization of a photoactive resin is triggered, leading to local increase in refractive index, which itself creates self-focusing waveguides and further polymerization of photoresin into long hydrogel microfilaments. Diameter and spacing of the microfilaments can be tuned from 2 to 30 mu m by changing the coherence length of the light beam. Microfilaments show outstanding cell instructive properties with fibroblasts, tenocytes, endothelial cells, and myoblasts, influencing cell alignment, nuclear deformation, and extracellular matrix deposition. FLight is compatible with multiple types of photoresins and allows for biofabrication of centimeter-scale hydrogel constructs with excellent cell viability within seconds (<10 s per construct). Multidirectional microfilaments are achievable within a single hydrogel construct by changing the direction of FLight projection, and complex multimaterial/multicellular tissue-engineered constructs are possible by sequentially exchanging the cell-laden photoresin. FLight offers a transformational approach to developing anisotropic tissues using photo-crosslinkable biomaterials.
Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing
Chansoria, Parth; Rizzo, Riccardo; Rütsche, Dominic; et al. (2024)
Chemical Reviews
Harnessing light for cross-linking of photoresponsive materials has revolutionized the field of 3D printing. A wide variety of techniques leveraging broad-spectrum light shaping have been introduced as a way to achieve fast and high-resolution printing, with applications ranging from simple prototypes to biomimetic engineered tissues for regenerative medicine. Conventional light-based printing techniques use cross-linking of material in a layer-by-layer fashion to produce complex parts. Only recently, new techniques have emerged which deploy multidirection, tomographic, light-sheet or filamented light-based image projections deep into the volume of resin-filled vat for photoinitiation and cross-linking. These Deep Vat printing (DVP) approaches alleviate the need for layer-wise printing and enable unprecedented fabrication speeds (within a few seconds) with high resolution (>10 μm). Here, we elucidate the physics and chemistry of these processes, their commonalities and differences, as well as their emerging applications in biomedical and non-biomedical fields. Importantly, we highlight their limitations, and future scope of research that will improve the scalability and applicability of these DVP techniques in a wide variety of engineering and regenerative medicine applications.
Structured Light Projection Using Image Guide Fibers for In Situ Photo-biofabrication
Chansoria, Parth; Winkelbauer, Michael; Zhang, Shipin; et al. (2025)
Advanced Materials
Light-based biofabrication techniques have revolutionized the field of tissue engineering and regenerative medicine. Specifically, the projection of structured light, where the spatial distribution of light is controlled at both macro and microscale, has enabled precise fabrication of complex three dimensional structures with high resolution and speed. However, despite tremendous progress, biofabrication processes are mostly limited to benchtop devices which limit the flexibility in terms of where the fabrication can occur. Here, a Fiber-assisted Structured Light (FaSt-Light) projection apparatus for rapid in situ crosslinking of photoresins is demonstrated. This approach uses image-guide fiber bundles which can project bespoke images at multiple wavelengths, enabling flexibility and spatial control of different photoinitiation systems and crosslinking chemistries and also the location of fabrication. Coupling of different sizes of fibers and different lenses attached to the fibers to project small (several mm) or large (several cm) images for material crosslinking is demonstrated. FaSt-Light allows control over the cross-section of the crosslinked resins and enables the introduction of microfilaments which can further guide cellular infiltration, differentiation, and anisotropic matrix production. The proposed approach can lead to a new range of in situ biofabrication techniques which improve the translational potential of photofabricated tissues and grafts.
Filamented hydrogels as tunable conduits for guiding neurite outgrowth
Liu, Hao; Puiggalí-Jou, Anna; Chansoria, Parth; et al. (2025)
Materials Today Bio
Anisotropic scaffolds with unidirectionally aligned fibers present an optimal solution for nerve tissue engineering and graft repair. This study investigates the application of filamented light (FLight) biofabrication to create hydrogel matrices featuring highly aligned microfilaments, facilitating neurite guidance and outgrowth from encapsulated chicken dorsal root ganglion (DRG) cells. FLight employs optical modulation instability (OMI) to rapidly and safely (<5 s) fabricate hydrogel constructs with precise microfilament alignment. The tunability of FLight matrices was demonstrated by adjusting four key parameters: stiffness, porosity, growth factor release, and incorporation of biological cues. Matrix stiffness was fine-tuned by varying the projection light dose, yielding matrices with stiffness ranging from 0.6 to 5.7 kPa. Optimal neurite outgrowth occurred at a stiffness of 0.6 kPa, achieving an outgrowth of 2.5 mm over 4 days. Matrix porosity was modified using diffraction gratings in the optical setup. While significant differences in neurite outgrowth and alignment were observed between bulk and FLight gels, further increases in porosity from 40 % to 70 % enhanced cell migration and axon bundling without significantly affecting maximal outgrowth. The incorporation of protein microcrystals containing nerve growth factor (NGF) into the photoresin enabled sustained neurite outgrowth without the need for additional NGF in the media. Finally, laminin was added to the resin to enhance the bioactivity of the biomaterial, resulting in a further increase in maximum neurite outgrowth to 3.5 mm after 4 days of culture in softer matrices. Overall, the varied matrix properties achieved through FLight significantly enhance neurite outgrowth, highlighting the importance of adaptable scaffold characteristics for guiding neurite development. This demonstrates the potential of FLight as a versatile platform for creating ideal matrices for clinical applications in nerve repair and tissue engineering.

Publications 1 - 10 of 15

Parth Chansoria

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