Tobias Bruderer


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Bruderer

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Tobias

ORCID

0000-0003-2399-2332

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2018 - 201952020 - 20213
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Publications 1 - 8 of 8
  • On-Line Analysis of Exhaled Breath
    Item type: Review Article
    Bruderer, Tobias; Gaisl, Thomas; Gaugg, Martin T.; et al. (2019)
    Chemical Reviews
  • Detection of volatile organic compounds with SESI and PTR high-resolution mass spectrometry: a feature comparison
    Item type: Journal Article
    Bruderer, Tobias; Gaugg, Martin T.; Cappellin, Luca; et al. (2020)
    Journal of the American Society for Mass Spectrometry
    The analysis of volatiles is of high relevance for a wide range of applications from environmental air sampling, security screening to potential medical applications. High-resolution mass spectrometry methods offer a particular wide compound coverage, sensitivity and selectivity. On-line approaches allow direct analysis in real time without the need for sample preparation. For the first time, we systematically compared the analysis of volatile organic compounds with Secondary Electrospray Ionization (SESI) and Proton Transfer Reaction (PTR) high-resolution mass spectrometers. The selected instruments had comparable mass resolving power with 15’000 m/Δm or higher, which is particularly suitable for non-targeted analysis, for example, of exhaled breath. Exhalations from 14 healthy adults were analyzed simultaneously on both instruments. In addition, 97 reference standards from nine chemical classes were analyzed with a liquid evaporation system. Surprisingly, in breath, we reported more complementary than overlapping features. A clear mass dependence was observed for each method with the highest number of detected m/z features for SESI in the high mass region (m/z = 150 – 250) and for PTR in the low mass region (m/z = 50 – 150). SESI yielded a significantly higher numbers of peaks (828) compared to PTR (491) among a total of 1304 unique breath m/z features. The number of signals observed by both methods was lower than expected (134 features) with 796 unique SESI features and 374 unique PTR features. Hypotheses to explain the observed mass-dependent differences are proposed. © 2020 American Chemical Society
  • Real-Time Breath Analysis Reveals Specific Metabolic Signatures of COPD Exacerbations
    Item type: Journal Article
    Gaugg, Martin T.; Nussbaumer-Ochsner, Yvonne; Bregy, Lukas; et al. (2019)
    Chest
  • Real-time exhaled breath analysis in patients with cystic fibrosis and controls
    Item type: Journal Article
    Gaisl, Thomas; Bregy, Lukas; Stebler, Nina; et al. (2018)
    Journal of Breath Research
  • Monitoring peppermint washout in the breath metabolome by secondary electrospray ionization-high resolution mass spectrometry
    Item type: Journal Article
    Lan, Jiayi; Gisler, Amanda; Bruderer, Tobias; et al. (2021)
    Journal of Breath Research
    In this study, a secondary electrospray ionization-high resolution mass spectrometer (SESI-HRMS) system was employed to profile the real-time exhaled metabolome of ten subjects who had ingested a peppermint oil capsule. In total, six time points were sampled during the experiment. Using an untargeted way of profiling breath metabolome, 2333 m/z unique metabolite features were determined in positive mode, and 1322 in negative mode. To benchmark the performance of the SESI-HRMS setup, several additional checks were done, including determination of the technical variation, the biological variation of one subject within three days, the variation within a time point, and the variation across all samples, taking all m/z features into account. Reproducibility was good, with the median technical variation being 18% and the median variation within biological replicates being 34%. Both variations were lower than the variation across individuals. Washout profiles of compounds from the peppermint oil, including menthone, limonene, pulegone, menthol and menthofuran were determined in all subjects. Metabolites of the peppermint oil were also determined in breath, for example, cis/trans-carveol, perillic acid and menthol glucuronide. Butyric acid was found to be the major metabolite that reduce the uptake rate of limonene. Pathways related to limonene metabolism were examined, and meaningful pathways were identified from breath metabolomics data acquired by SESI using an untargeted analysis. © IOP Publishing Ltd. 2020
  • Molecular breath analysis supports altered amino acid metabolism in idiopathic pulmonary fibrosis
    Item type: Journal Article
    Gaugg, Martin T.; Engler, Anna; Bregy, Lukas; et al. (2019)
    Respirology
  • Metabolic changes during periodontitis therapy assessed by real-time ambient mass spectrometry
    Item type: Journal Article
    Bregy, Lukas; Hirsiger, Constanze; Gartenmann, Stefanie; et al. (2019)
    Clinical Mass Spectrometry
  • Volatile organic compound breath signatures of children with cystic fibrosis by real-time SESI-HRMS
    Item type: Journal Article
    Weber, Ronja; Haas, Naemi; Baghdasaryan, Astghik; et al. (2020)
    ERJ Open Research
    Early pulmonary infection and inflammation result in irreversible lung damage and are major contributors to cystic fibrosis (CF)-related morbidity. An easy to apply and noninvasive assessment for the timely detection of disease-associated complications would be of high value. We aimed to detect volatile organic compound (VOC) breath signatures of children with CF by real-time secondary electrospray ionisation high-resolution mass spectrometry (SESI-HRMS). A total of 101 children, aged 4–18 years (CF=52; healthy controls=49) and comparable for sex, body mass index and lung function were included in this prospective cross-sectional study. Exhaled air was analysed by a SESI-source linked to a high-resolution time-of-flight mass spectrometer. Mass spectra ranging from m/z 50 to 500 were recorded. Out of 3468 m/z features, 171 were significantly different in children with CF (false discovery rate adjusted p-value of 0.05). The predictive ability (CF versus healthy) was assessed by using a support-vector machine classifier and showed an average accuracy (repeated cross-validation) of 72.1% (sensitivity of 77.2% and specificity of 67.7%). This is the first study to assess entire breath profiles of children with SESI-HRMS and to extract sets of VOCs that are associated with CF. We have detected a large set of exhaled molecules that are potentially related to CF, indicating that the molecular breath of children with CF is diverse and informative.
Publications 1 - 8 of 8