Patrick Misun


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Misun

First Name

Patrick

ORCID

0000-0003-1050-5742

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2013 - 2019202020 - 202412
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Publications1 - 10 of 32
  • Biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks
    Item type: Other Conference Item
    Misun, Patrick; Rothe, Jörg; Hierlemann, Andreas; et al. (2016)
    3D Cell Culture 2016: How close to "in vivo" can we get?: Models, applications & translation: Programme and book of abstracts: 19-21 April 2016, Konzerthaus Freiburg, Germany
  • Investigating 3D microtissue metabolism using hanging drops with integrated biosensors
    Item type: Other Conference Item
    Rousset, Nassim; Hierlemann, Andreas; Misun, Patrick (2020)
  • Microfluidic hanging-drop platforms for culture, interaction and analysis of 3D microtissues
    Item type: Other Conference Item
    Misun, Patrick; Boos, Julia A.; Rousset, Nassim; et al. (2019)
  • Microphysiological Fluidic Hanging Drop Network for Multi-Tissue Interaction and Analysis
    Item type: Conference Paper
    Misun, Patrick; Rismani Yazdi, Saeed; Bürgel, Sebastian; et al. (2016)
  • Seamless combination of FACS and hanging-drop networks for individual handling and culturing of stem cells and microtissue spheroids
    Item type: Journal Article
    Birchler, Axel; Berger, Mischa; Jäggin, Verena; et al. (2016)
    Analytical Chemistry
  • Spontaneous neuronal activity in developing mouse cerebral organoids
    Item type: Other Conference Item
    Schröter, Manuel; Girr, Monika; Boos, Julia A.; et al. (2018)
    Neuroscience 2018 Abstracts
  • Microfluidic multi-tissue platform for systemic embryotoxicity testing along the maternal-placental-embryonic axis
    Item type: Conference Paper
    Boos, Julia A.; Misun, Patrick; Brunoldi, Giulia; et al. (2022)
    25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
    A microfluidic co-culture platform for systemic embryotoxicity testing along the maternal-placental-embryonic axis is presented. We advanced the hanging-drop network (HDN) technology by combining a 2D placental barrier with 3D embryoid bodies (EBs) in immediate vicinity in a single microfluidic network. In contrast to previous approaches, the newly developed platform does not only allow for studying effects of direct toxicity on the individual tissue models but also enables the investigation of indirect embryotoxicity effects, mediated by factors secreted by the placental tissue. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
  • An Immunocompetent Microphysiological System to Simultaneously Investigate Effects of Anti-Tumor Natural Killer Cells on Tumor and Cardiac Microtissues
    Item type: Journal Article
    Nguyen, Thi Phuong Oanh; Misun, Patrick; Lohasz, Christian; et al. (2021)
    Frontiers in Immunology
    Existing first-line cancer therapies often fail to cope with the heterogeneity and complexity of cancers, so that new therapeutic approaches are urgently needed. Among novel alternative therapies, adoptive cell therapy (ACT) has emerged as a promising cancer treatment in recent years. The limited clinical applications of ACT, despite its advantages over standard-of-care therapies, can be attributed to (i) time-consuming and cost-intensive procedures to screen for potent anti-tumor immune cells and the corresponding targets, (ii) difficulties to translate in-vitro and animal-derived in-vivo efficacies to clinical efficacy in humans, and (iii) the lack of systemic methods for the safety assessment of ACT. Suitable experimental models and testing platforms have the potential to accelerate the development of ACT. Immunocompetent microphysiological systems (iMPS) are microfluidic platforms that enable complex interactions of advanced tissue models with different immune cell types, bridging the gap between in-vitro and in-vivo studies. Here, we present a proof-of-concept iMPS that supports a triple culture of three-dimensional (3D) colorectal tumor microtissues, 3D cardiac microtissues, and human-derived natural killer (NK) cells in the same microfluidic network. Different aspects of tumor-NK cell interactions were characterized using this iMPS including: (i) direct interaction and NK cell-mediated tumor killing, (ii) the development of an inflammatory milieu through enrichment of soluble pro-inflammatory chemokines and cytokines, and (iii) secondary effects on healthy cardiac microtissues. We found a specific NK cell-mediated tumor-killing activity and elevated levels of tumor- and NK cell-derived chemokines and cytokines, indicating crosstalk and development of an inflammatory milieu. While viability and morphological integrity of cardiac microtissues remained mostly unaffected, we were able to detect alterations in their beating behavior, which shows the potential of iMPS for both, efficacy and early safety testing of new candidate ACTs.
  • Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks
    Item type: Journal Article
    Misun, Patrick; Rothe, Jörg; Schmid, Yannick R.F.; et al. (2016)
    Microsystems & Nanoengineering
    Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out functionalities. Here we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for ‘body-on-a-chip’ applications. The microfluidic platform is based on the technology of hanging-drop networks, which is designed for the formation, cultivation, and analysis of fluidically interconnected organotypic spherical three-dimensional (3D) microtissues of multiple cell types. The sensor modules were designed as small glass plug-ins featuring four platinum working electrodes, a platinum counter electrode, and an Ag/AgCl reference electrode. They were placed directly into the ceiling substrate from which the hanging drops that host the spheroid cultures are suspended. The electrodes were functionalized with oxidase enzymes to enable continuous monitoring of lactate and glucose through amperometry. The biosensors featured high sensitivities of 322±41 nA mM−1 mm−2 for glucose and 443±37 nA mM−1 mm−2 for lactate; the corresponding limits of detection were below 10 μM. The proposed technology enabled tissue-size-dependent, real-time detection of lactate secretion from single human colon cancer microtissues cultured in the hanging drops. Furthermore, glucose consumption and lactate secretion were monitored in parallel, and the impact of different culture conditions on the metabolism of cancer microtissues was recorded in real-time.
  • Investigating the intercellular interaction between 3D gut epithelial microtissues and circulating MAIT cells using a microfluidic tilting platform
    Item type: Other Conference Item
    Nguyen, Thi Phuong Oanh; Misun, Patrick; Lohasz, Christian; et al. (2019)
    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
    Microphysiological systems constitute new, versatile tools to investigate the pathogenesis of different immunemediated inflammatory diseases – among them inflammatory bowel disease (IBD). To recapitulate more faithfully the interaction between the gut model and relevant immune cells, we adapted a commercialized microfluidic platform to co-culture three-dimensional (3D) gut epithelial microtissues (MTs) with circulating mucosalassociated invariant T (MAIT) cells in vitro in a dynamic microenvironment. This platform enables i) on-chip optical readout of specific physical interaction between 3D gut epithelial MTs and MAIT cells, and ii) supernatantbased readout of soluble cytokine signals in both, healthy and infection scenarios.
Publications1 - 10 of 32