Nicola Zamboni

Last Name

Zamboni

First Name

Nicola

ORCID

0000-0003-1271-1021

Organisational unit

03713 - Sauer, Uwe / Sauer, Uwe

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Publications1 - 10 of 180

Metabolic control of adult neural stem cell activity by Fasn-dependent lipogenesis
Knobloch, Marlen; Braun, Simon M. G.; Zurkirchen, Luis; et al. (2013)
Nature
Alternate-day fasting elicits larger changes in fat mass than time-restricted eating in adults without obesity – A randomized clinical trial
Derron, Nina; Güntner, Andreas; Weber, Ines; et al. (2025)
Clinical Nutrition
Background & aims Intermittent fasting (IF) is a popular nutritional strategy for weight control and improved metabolic health, however it is unclear which type of intermittent fasting is most effective. This randomized trial directly compared short-term alternate-day fasting (ADF) and time-restricted eating (TRE) with controls in adults with overweight or a high normal weight. The aim was to compare the effects of ADF and TRE versus controls regarding whole-body fat mass loss, weight control and cardiometabolic health. Methods In this 4-week, parallel-arm, randomized clinical trial (February 2021–May 2022), participants aged 18–40 years with a body mass index between 23 and 30 kg/m2 were assigned to ADF (alternating fasting and ad libitum eating days), to TRE (eating only between 12:00–20:00), or control (no change in eating times). The primary outcome was change in total fat volume (assessed by whole-body magnetic resonance imaging). Secondary outcomes were subcutaneous and visceral fat mass, body weight, resting metabolic rate, biochemical markers, energy intake, activity energy expenditure and health-related quality of life. Results Seventy-six participants (mean [standard deviation (SD)] age, 29.6 [5.6] years; body mass index, 25.8 [2.2] kg/m2; 34 [44 %] female) were randomized to ADF (n = 26), TRE (n = 26), or control (n = 24). Seventy-five participants completed the trial (25 in ADF, 26 in TRE, 24 in control). ADF led to a greater reduction in total fat volume than control (mean difference −1059.8 cm3, 95 % CI: −1380.0 cm3 to −739.6 cm3, p < 0.001) and TRE (−695.7 cm3, 95 % CI: −1013.9 to −377.6 cm3, p < 0.001). TRE also reduced total fat volume compared to control (−364.0 cm3, 95 % CI: −621.3 cm3 to −106.7 cm3, p = 0.007). Energy intake was reduced by 34 % [18 %] in ADF, 15 % [21 %] in TRE and 3 % [22 %] in control. ADF, but not TRE, reduced visceral fat mass, resting metabolic rate, triiodothyronine and non-HDL cholesterol compared to controls. Only ADF increased activity energy expenditure and health-related quality of life. No serious adverse events occurred. Conclusions In this randomized clinical trial, ADF was more effective in reducing energy intake than TRE which has subsequent effects on fat mass and body weight. Only ADF improved several cardiometabolic risk factors.
Paraburkholderia phymatum Homocitrate Synthase NifV Plays a Key Role for Nitrogenase Activity during Symbiosis with Papilionoids and in Free-Living Growth Conditions
Bellés-Sancho, Paula; Lardi, Martina; Liu, Yilei; et al. (2021)
Cells
Homocitrate is an essential component of the iron-molybdenum cofactor of nitrogenase, the bacterial enzyme that catalyzes the reduction of dinitrogen (N2) to ammonia. In nitrogen-fixing and nodulating alpha-rhizobia, homocitrate is usually provided to bacteroids in root nodules by their plant host. In contrast, non-nodulating free-living diazotrophs encode the homocitrate synthase (NifV) and reduce N2 in nitrogen-limiting free-living conditions. Paraburkholderia phymatum STM815 is a beta-rhizobial strain, which can enter symbiosis with a broad range of legumes, including papilionoids and mimosoids. In contrast to most alpha-rhizobia, which lack nifV, P. phymatum harbors a copy of nifV on its symbiotic plasmid. We show here that P. phymatum nifV is essential for nitrogenase activity both in root nodules of papilionoid plants and in free-living growth conditions. Notably, nifV was dispensable in nodules of Mimosa pudica despite the fact that the gene was highly expressed during symbiosis with all tested papilionoid and mimosoid plants. A metabolome analysis of papilionoid and mimosoid root nodules infected with the P. phymatum wild-type strain revealed that among the approximately 400 measured metabolites, homocitrate and other metabolites involved in lysine biosynthesis and degradation have accumulated in all plant nodules compared to uninfected roots, suggesting an important role of these metabolites during symbiosis.
Cross-platform comparison of methods for quantitative metabolomics of primary metabolism
Büscher, Jörg Martin; Czernik, Dominika; Ewald, Jennifer Christina; et al. (2009)
Analytical Chemistry
Regularized adversarial learning for normalization of multi-batch untargeted metabolomics data
Dmitrenko, Andrei; Reid, Michelle; Zamboni, Nicola (2023)
Bioinformatics
Motivation: Untargeted metabolomics by mass spectrometry is the method of choice for unbiased analysis of molecules in complex samples of biological, clinical or environmental relevance. The exceptional versatility and sensitivity of modern high-resolution instruments allows profiling of thousands of known and unknown molecules in parallel. Inter-batch differences constitute a common and unresolved problem in untargeted metabolomics, and hinder the analysis of multi-batch studies or the intercomparison of experiments. Results: We present a new method, Regularized Adversarial Learning Preserving Similarity (RALPS), for the normal- ization of multi-batch untargeted metabolomics data. RALPS builds on deep adversarial learning with a three-term loss function that mitigates batch effects while preserving biological identity, spectral properties and coefficients of variation. Using two large metabolomics datasets, we showcase the superior performance of RALPS as compared with six state-of-the-art methods for batch correction. Further, we demonstrate that RALPS scales well, is robust, deals with missing values and can handle different experimental designs. Availability and implementation: https://github.com/zamboni-lab/RALPS. Contact: nzamboni@ethz.ch Supplementary information: Supplementary data are available at Bioinformatics online.
Genetics
Sauer, Uwe; Heinemann, Matthias; Zamboni, Nicola (2007)
Science
High-throughput discovery metabolomics
Fuhrer, Tobias; Zamboni, Nicola (2015)
Current Opinion in Biotechnology
Dietary excess regulates absorption and surface of gutepithelium through intestinal PPARα
Stojanović, Ozren; Altirriba, Jordi; Rigo, Dorothée; et al. (2021)
Nature Communications
Intestinal surface changes in size and function, but what propels these alterations and what are their metabolic consequences is unknown. Here we report that the food amount is a positive determinant of the gut surface area contributing to an increased absorptive function, reversible by reducing daily food. While several upregulated intestinal energetic pathways are dispensable, the intestinal PPAR alpha is instead necessary for the genetic and environment overeating-induced increase of the gut absorptive capacity. In presence of dietary lipids, intestinal PPAR alpha knock-out or its pharmacological antagonism suppress intestinal crypt expansion and shorten villi in mice and in human intestinal biopsies, diminishing the postprandial triglyceride transport and nutrient uptake. Intestinal PPAR alpha ablation limits systemic lipid absorption and restricts lipid droplet expansion and PLIN2 levels, critical for droplet formation. This improves the lipid metabolism, and reduces body adiposity and liver steatosis, suggesting an alternative target for treating obesity.
L-Arginine Modulates T Cell Metabolism and Enhances Survival and Anti-tumor Activity
Geiger, Roger; Rieckmann, Jan C.; Wolf, Tobias; et al. (2016)
Cell
Metabolic activity is intimately linked to T cell fate and function. Using high-resolution mass spectrometry, we generated dynamic metabolome and proteome profiles of human primary naive T cells following activation. We discovered critical changes in the arginine metabolism that led to a drop in intracellular L-arginine concentration. Elevating L-arginine levels induced global metabolic changes including a shift from glycolysis to oxidative phosphorylation in activated T cells and promoted the generation of central memory-like cells endowed with higher survival capacity and, in a mouse model, anti-tumor activity. Proteome-wide probing of structural alterations, validated by the analysis of knockout T cell clones, identified three transcriptional regulators (BAZ1B, PSIP1, and TSN) that sensed L-arginine levels and promoted T cell survival. Thus, intracellular L-arginine concentrations directly impact the metabolic fitness and survival capacity of T cells that are crucial for anti-tumor responses.
D-Glucosamine supplementation extends life span of nematodes and of ageing mice
Weimer, Sandra; Priebs, Josephine; Kuhlow, Doreen; et al. (2014)
Nature Communications
D-Glucosamine (GlcN) is a freely available and commonly used dietary supplement potentially promoting cartilage health in humans, which also acts as an inhibitor of glycolysis. Here we show that GlcN, independent of the hexosamine pathway, extends Caenorhabditis elegans life span by impairing glucose metabolism that activates AMP-activated protein kinase (AMPK/AAK-2) and increases mitochondrial biogenesis. Consistent with the concept of mitohormesis, GlcN promotes increased formation of mitochondrial reactive oxygen species (ROS) culminating in increased expression of the nematodal amino acid-transporter 1 (aat-1) gene. Ameliorating mitochondrial ROS formation or impairment of aat-1-expression abolishes GlcN-mediated life span extension in an NRF2/SKN-1-dependent fashion. Unlike other calorie restriction mimetics, such as 2-deoxyglucose, GlcN extends life span of ageing C57BL/6 mice, which show an induction of mitochondrial biogenesis, lowered blood glucose levels, enhanced expression of several murine amino-acid transporters, as well as increased amino-acid catabolism. Taken together, we provide evidence that GlcN extends life span in evolutionary distinct species by mimicking a low-carbohydrate diet.

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Nicola Zamboni

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