Sebastian Wolf

Last Name

Wolf

First Name

Sebastian

ORCID

0000-0001-7717-6993

Organisational unit

09732 - Carminati, Andrea / Carminati, Andrea

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Publications1 - 10 of 42

Epigenetic control over the cell-intrinsic immune response antagonizes self-renewal in acute myeloid leukemia
Felipe Fumero, Eloísa; Walter, Carolin; Frenz, Joris Maximillian; et al. (2024)
Blood
Epigenetic modulation of the cell-intrinsic immune response holds promise as a therapeutic approach for leukemia. However, current strategies designed for transcriptional activation of endogenous transposons and subsequent interferon type-I (IFN-I) response, show limited clinical efficacy. Histone lysine methylation is an epigenetic signature in IFN-I response associated with suppression of IFN-I and IFN-stimulated genes, suggesting histone demethylation as key mechanism of reactivation. In this study, we unveil the histone demethylase PHF8 as a direct initiator and regulator of cell-intrinsic immune response in acute myeloid leukemia (AML). Site-specific phosphorylation of PHF8 orchestrates epigenetic changes that upregulate cytosolic RNA sensors, particularly the TRIM25-RIG-I-IFIT5 axis, thereby triggering the cellular IFN-I response-differentiation-apoptosis network. This signaling cascade largely counteracts differentiation block and growth of human AML cells across various disease subtypes in vitro and in vivo. Through proteome analysis of over 200 primary AML bone marrow samples, we identify a distinct PHF8/IFN-I signature in half of the patient population, without significant associations with known clinically or genetically defined AML subgroups. This profile was absent in healthy CD34+ hematopoietic progenitor cells, suggesting therapeutic applicability in a large fraction of patients with AML. Pharmacological support of PHF8 phosphorylation significantly impairs the growth in samples from patients with primary AML. These findings provide novel opportunities for harnessing the cell-intrinsic immune response in the development of immunotherapeutic strategies against AML.
Author Correction: Global influence of soil texture on ecosystem water limitation
Wankmüller, Fabian; Delval, Louis; Lehmann Grunder, Peter Ulrich; et al. (2025)
Nature
In the version of this article initially published, incorrect values were shown in the “hb/[cm]” column of Supplementary Table 1, and the last sentence of the legend for Extended Data Fig. 3 had an error where “pale colours” now replaces “dotted area, AI > 1” in the description. The colours for loamy sand and clay in Fig. 1 have been updated to match usage in other figures, while the full names of the authors have now been spelled out. The changes have been made in the Supplementary information and HTML and PDF versions of the article.
The FLUXNET2015 dataset and the ONEFlux processing pipeline for eddy covariance data
Pastorello, Gilberto Z.; Trotta, Carlo; Canfora, Eleonora; et al. (2020)
Scientific Data
The FLUXNET2015 dataset provides ecosystem-scale data on CO2, water, and energy exchange between the biosphere and the atmosphere, and other meteorological and biological measurements, from 212 sites around the globe (over 1500 site-years, up to and including year 2014). These sites, independently managed and operated, voluntarily contributed their data to create global datasets. Data were quality controlled and processed using uniform methods, to improve consistency and intercomparability across sites. The dataset is already being used in a number of applications, including ecophysiology studies, remote sensing studies, and development of ecosystem and Earth system models. FLUXNET2015 includes derived-data products, such as gap-filled time series, ecosystem respiration and photosynthetic uptake estimates, estimation of uncertainties, and metadata about the measurements, presented for the first time in this paper. In addition, 206 of these sites are for the first time distributed under a Creative Commons (CC-BY 4.0) license. This paper details this enhanced dataset and the processing methods, now made available as open-source codes, making the dataset more accessible, transparent, and reproducible.
Partitioning evapotranspiration with concurrent eddy covariance measurements in a mixed forest
Paul-Limoges, Eugénie; Wolf, Sebastian; Haghighi, Erfan; et al. (2019)
Geophysical Research Abstracts
Plants have an important effect on our climate: by assimilating CO2 through photosynthesis, plants mitigate increases in atmospheric CO2 concentrations, transfer water to the atmosphere and reduce surface temperatures. As atmospheric CO2 concentration increases, plants can reduce the opening of their stomata and still enhance photosynthesis. This CO2 fertilization effect can lead to increased growth but also to reduced transpiration rates, thereby contributing to warmer surface temperatures. There is ongoing debate on how these contrasting effects will influence our climate, in particular the contribution of forest ecosystems. It is however difficult to measure transpiration to understand these changes. Direct eddy covariance (EC) measurements are currently the best available approach to directly observe interactions linked to biosphere-atmosphere CO2 and water vapor exchange. While there are well-established methods to partition CO2 fluxes into the component fluxes of photosynthesis and respiration, there is still no standardized method to partition water vapor fluxes (evapotranspiration) into the component fluxes of evaporation and transpiration. In this study, we are using two years of concurrent below and above canopy EC measurements in a mixed deciduous forest in Switzerland to partition water vapor fluxes into the biological transpiration and physical evaporation. We compare our results with evaporation modeled with the Haghighi & Or (HO) model, and transpiration obtained from two models, namely the ecosystem demographic (ED2) and the Haghighi & Kirchner (HK) transpiration models, as well as derived from plot-level sap flow measurements. We found that transpiration accounted on average for 80% of evapotranspiration, emphasizing a considerably lower contribution from evaporation. Mid-afternoon reductions in transpiration were found during periods of high vapor pressure deficit in summer. Despite pronounced differences in environmental conditions during the observed years, evapotranspiration and its transpiration component had a low inter-annual variability in this mixed forest. These results suggest a reliable access of the trees to water resources and stomatal regulation in response to enhanced atmospheric evaporative demand. Our results show the potential of concurrent below and above canopy EC measurements to partition evapotranspiration in forested areas.
Spatio-Temporal Convergence of Maximum Daily Light-Use Efficiency Based on Radiation Absorption by Canopy Chlorophyll
Zhang, Yao; Xiao, Xiangming; Wolf, Sebastian; et al. (2018)
Geophysical Research Letters
Increased photosynthesis during spring drought in energy-limited ecosystems
Miller, David L.; Wolf, Sebastian; Fisher, Joshua B.; et al. (2023)
Nature Communications
Drought is often thought to reduce ecosystem photosynthesis. However, theory suggests there is potential for increased photosynthesis during meteorological drought, especially in energy-limited ecosystems. Here, we examine the response of photosynthesis (gross primary productivity, GPP) to meteorological drought across the water-energy limitation spectrum. We find a consistent increase in eddy covariance GPP during spring drought in energy-limited ecosystems (83% of the energy-limited sites). Half of spring GPP sensitivity to precipitation was predicted solely from the wetness index (R2 = 0.47, p < 0.001), with weaker relationships in summer and fall. Our results suggest GPP increases during spring drought for 55% of vegetated Northern Hemisphere lands ( >30° N). We then compare these results to terrestrial biosphere model outputs and remote sensing products. In contrast to trends detected in eddy covariance data, model mean GPP always declined under spring precipitation deficits after controlling for air temperature and light availability. While remote sensing products captured the observed negative spring GPP sensitivity in energy-limited ecosystems, terrestrial biosphere models proved insufficiently sensitive to spring precipitation deficits.
Understanding the controls over forest carbon use efficiency on small spatial scales: Effects of forest disturbance and tree diversity
Kunert, Norbert; El-Madany, Tarek S.; Aparecido, Luiza M.T.; et al. (2019)
Agricultural and Forest Meteorology
Dynamics of Evapotranspiration Partitioning with Concurrent Subcanopy Flux Measurements in a Montane Sierra Nevada Forest
Wolf, Sebastian; Kirchner, James W. (2019)
Geophysical Research Abstracts
The component fluxes of Evapotranspiration (ET) cannot be directly measured at the ecosystem scale, and standardized approaches to partition the components fluxes of eddy-covariance (EC) measured ET are currently not available. Concurrent measurements of below and above canopy EC in forests provide a promising approach. However, our understanding of the performance of such measurements is still very limited as questions remain regarding the spatial variability, canopy (de-)coupling, and the temporal dynamics of subcanopy EC measurements. To address these challenges, we measured and partitioned ET with two subcanopy and concurrent above canopy EC towers in a montane forest at Sagehen Creek (California), having a pronounced Mediterranean climate. We analysed these paired measurements for 15 months and will present the spatial and temporal dynamics of this flux partitioning approach. Based on these results, we will discuss the relevant process controls, methodological limitations and implications for a wider application of such concurrent measurements.
Temperature thresholds of ecosystem respiration at a global scale
Johnston, Alice S.A.; Wolf, Sebastian; et al. (2021)
Nature Ecology & Evolution
Ecosystem respiration is a major component of the global terrestrial carbon cycle and is strongly influenced by temperature. The global extent of the temperature–ecosystem respiration relationship, however, has not been fully explored. Here, we test linear and threshold models of ecosystem respiration across 210 globally distributed eddy covariance sites over an extensive temperature range. We find thresholds to the global temperature–ecosystem respiration relationship at high and low air temperatures and mid soil temperatures, which represent transitions in the temperature dependence and sensitivity of ecosystem respiration. Annual ecosystem respiration rates show a markedly reduced temperature dependence and sensitivity compared to half-hourly rates, and a single mid-temperature threshold for both air and soil temperature. Our study indicates a distinction in the influence of environmental factors, including temperature, on ecosystem respiration between latitudinal and climate gradients at short (half-hourly) and long (annual) timescales. Such climatological differences in the temperature sensitivity of ecosystem respiration have important consequences for the terrestrial net carbon sink under ongoing climate change.
Dynamics of evapotranspiration from concurrent above- and below-canopy flux measurements in a montane Sierra Nevada forest
Wolf, Sebastian; Paul-Limoges, Eugénie; Sayler, Dan; et al. (2024)
Agricultural and Forest Meteorology
Evapotranspiration (ET) from the land surface to the atmosphere is a major component of Earth's water cycle, and comprises both transpiration (T) of xylem water from plants and evaporation (E) of water from soils and vegetation surfaces. These two component fluxes respond differently to changes in temperature, water availability and atmospheric CO2 concentrations. Concurrent eddy covariance (EC) measurements above and below forest canopies provide a promising approach to partition ET into E and T. However, below-canopy EC measurements are rare, and questions remain regarding their spatial variability, canopy coupling, and temporal dynamics. To address these challenges, we measured and partitioned ET over more than three years, using concurrent above- and below-canopy EC towers in a montane forest at Sagehen Creek in California's Sierra Nevada mountains. This is the establishing study for the AmeriFlux site US-SHC. The main environmental control for ET was available energy; other important controls were canopy & soil temperature, soil moisture, vapor pressure deficit, and wind speed. Below-canopy measurements at two locations within the above-canopy footprint were similar to one another, suggesting low spatial heterogeneity in understory ET near the creek at our Sagehen site. We observed a total forest ET of 606 ± 50 mm yr−1 with 275 ± 17 mm yr−1 measured in the understory (all mean ± SD) during the water years 2018–2020. Interannual variability in ET and T was small despite large variability in precipitation totals; thus the P–ET water balance was mainly driven by variations in water supply. Partitioning the components of total forest ET at Sagehen with concurrent EC measurements showed that on average, 67–74% of ET originated from T (47% from trees and 20–27% from understory vegetation), while 26–33% were from E (mostly from the understory). Our results demonstrate the potential of concurrent above- and below-canopy EC measurements for ET partitioning.

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Sebastian Wolf

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