Longfeng Rao


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Rao

First Name

Longfeng

ORCID

0000-0002-2139-4972

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2022 - 20256
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Publications 1 - 6 of 6
  • Towards understanding knee instability after TKA from a biomechanical perspective
    Item type: Doctoral Thesis
    Rao, Longfeng (2023)
  • Can tibio-femoral kinematic and kinetic parameters reveal poor functionality and underlying deficits after total knee replacement? A systematic review
    Item type: Review Article
    Rao, Longfeng; Taylor, William R.; Horn, Nils; et al. (2022)
    The Knee
    Background Extensive efforts have been made to understand joint kinematics and kinetics in total knee arthroplasty (TKA) in subjects with satisfactory outcomes during daily functional activities and clinical tests, but it remains unclear whether such movement characteristics hold the potential to indicate the underlying aetiology of unsatisfactory or bad TKA outcomes. Purpose To investigate which kinematic and kinetic parameters assessed during passive clinical tests and functional activities of daily living are associated with poor functionality and underlying deficits after total knee replacement. Methods We focused on studies characterizing the kinematic or kinetic parameters of the knee joint that are associated with poor clinical outcome after TKA. Seventeen articles were included for the review, and kinematic and kinetic data from 719 patients with minimal follow up of 6 months were extracted and analyzed. Results Passive posterior translation at 90° flexion exhibited good potential for differentiating stable and unstable TKAs. Anterior-posterior (A-P) translation of the medial condyle at 0–30° and 30–60° flexion, A-P translation of the lateral condyle at 60–90° during closed chain exercises, as well as knee extension moment during stair ascent and descent, knee abduction moment during stair descent, knee internal rotation moment and plantar flexion moment during walking, 2nd peak ground reaction force during stair ascent and walking showed the greatest promise as functional biomarkers for a dissatisfied/poor outcome knee after TKA. Conclusion In this study, we systematically reviewed the state-of-the-art knowledge of kinematics and kinetics associated with functional deficits, and found 11 biomechanical parameters that showed promise for supporting decision making in TKA.
  • Passive Anterior-Posterior and Rotational Laxity are Indicative of Subjective Knee Instability following TKA with Ultra-Congruent Inserts
    Item type: Other Conference Item
    Rao, Longfeng; Horn, Nils; Preiss, Stefan; et al. (2022)
  • Understanding Knee Instability after TKA by means of Dynamic Video-fluoroscopy
    Item type: Other Conference Item
    Rao, Longfeng; Meister, Nadja; Horn, Nils; et al. (2022)
    ESB 2022 Abstract Book
  • Nasal Chondrocytes Intensively Invade and Repair Pathologically Altered Cartilage Through Intrinsic Genomic Mechanisms: A Narrative Review
    Item type: Review Article
    Shestakova, Victoria A.; Smirnova, Ekaterina I.; Rao, Longfeng; et al. (2025)
    Current Rheumatology Reviews
    Articular cartilage, a crucial component of joint structure, ensures smooth articulation and efficient load distribution within the joint. However, its integrity is compromised in various pathological conditions, such as osteoarthritis, leading to significant alterations in its structure and function. This process was significantly correlated with Extracellular Matrix (ECM) degradation, loss of collagen type II, and increased expression of matrix metalloproteinases (MMPs), particularly MMP-13. The ability of chondrocytes to invade into the ECM in pathologically altered tissue leads to cartilage repair and regeneration, and becomes the basis of chondrocyte cell therapy. Furthermore, the altered mechanical properties of the ECM in diseased cartilage, alongside the upregulation of chemotactic factors, contribute to the enhanced migratory behavior of chondrocytes. Interestingly, chondrocytes invading the ECM displayed signs of phenotypic changes, such as increased proliferation and expression of markers associated with chondrocytes' intrinsic genetic properties. The invasion of chondrocytes into the ECM is a response to cartilage damage, possibly driven by an attempt to repair the degraded ECM, and varies in chondrocytes from different sources, i.e., articular cartilage or nasal septum. Nasal chondrocytes highlight the increase of ACAN, SOX9, N-cadherin, COL2A expression and decrease of IL1B, CXCL8, and MMPs gene family expression, which could relate to their unique phenotype properties. However, this response may paradoxically contribute to the progression of cartilage pathology by disrupting the tissue architecture and promoting further degeneration. Our review highlights the endogenous genetic properties of nasal chondrocytes to invade and repair damaged cartilage, offering promising avenues for cartilage repair and regeneration.
  • Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study
    Item type: Journal Article
    Shestakova, Victoria A.; Klabukov, Ilya D.; Kolobaev, Ilya V.; et al. (2024)
    Knee Surgery & Related Research
    Background: Due to non-vascularized and aneural structure, articular cartilage has limited self-repairing capacity. The aim of this study was to investigate the revitalization of inflammatory injured articular cartilage matrices by human nasal chondrocytes (hNC). Materials and methods: Cartilage matrix was prepared by devitalization of articular cartilage samples obtained intraoperatively from an adult patient undergoing knee joint replacement. hNC were obtained from native tissues by enzymatic digestion with further expansion over two passages. The obtained nasal chondrocytes were used to seed decellularized scaffolds, which were then cultured in vitro for 7, 14, or 21 days in chondrogenic medium. Migration was observed by histologic staining with fast green, safranin-O, and hematoxylin and scanning electron microscopy. Biochemical analysis was performed to determine the glycosaminoglycan (GAG) and DNA content of the cartilage using dimethylmethylene blue and CyQuant Cell Proliferation Assay Kit. Results: We seeded healthy and inflamed cartilage with nasal chondrocytes and found that the cells actively invade mainly pathologically altered cartilage. The results of biochemical quantitative analysis showed that the amount of DNA significantly increased by day 7 and decreased by day 14, while the quantitative values of GAGs had the opposite trend. Histological staining showed that cartilage formation occurred on day 7, intercellular spaces were filled with de novo synthesized cartilage matrix with significantly low GAG content on day 14, and newly formed GAG-rich cartilage was observed on day 21. The obtained data on cartilage regeneration were confirmed by scanning electron microscopy. Conclusions: Our preliminary results showed that human nasal chondrocytes are capable of infiltrating the pathologically altered extracellular matrix of articular cartilage damaged by arthritis, thereby promoting its repair to a physiologically relevant state.
Publications 1 - 6 of 6