Goran Lovric

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Lovric

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Goran

ORCID

0000-0002-0833-4043

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Publications 1 - 10 of 15

Synchrotron X-Ray-Based Functional and Anatomical Lung Imaging Techniques
Bayat, Sam; Dullin, Christian; Kitchen, Marcus J.; et al. (2018)
Fundamental Biomedical Technologies ~ Advanced High-Resolution Tomography in Regenerative Medicine
Simultaneous Quantitation of Cationic and Non-ionic Contrast Agents in Articular Cartilage Using Synchrotron MicroCT Imaging
Saukko, Annina E.A.; Turunen, Mikael J.; Honkanen, Miitu K.M; et al. (2019)
Scientific Reports
Early diagnosis of acute cartilage injuries enables monitoring of disease progression and improved treatment option planning to prevent post-traumatic osteoarthritis. In contrast-enhanced computed tomography (CECT), the changes in cationic agent diffusion within the tissue reflect cartilage degeneration. The diffusion in degenerated cartilage depends on proteoglycan (PG) content and water content, but each having an opposite effect on diffusion, thus compromising the diagnostic sensitivity. To overcome this limitation, we propose the simultaneous imaging of cationic (sensitive to PG and water contents) and non-ionic (sensitive to water content) agents. In this study, quantitative dual-energy CT (QDECT) imaging of two agents is reported for the first time at clinically feasible imaging time points. Furthermore, this is the first time synchrotron microCT with monochromatic X-rays is employed in cartilage CECT. Imaging was conducted at 1 and 2 h post contrast agent immersion. Intact, PG-depleted, and mechanically injured + PG-depleted cartilage samples (n = 33) were imaged in a mixture of cationic (iodine-based CA4+) and non-ionic (gadolinium-based gadoteridol) agents. Concurrent evaluation of CA4+ and gadoteridol partitions in cartilage is accomplished using QDECT. Subsequent normalization of the CA4+ partition with that of the gadoteridol affords CA4+ attenuations that significantly correlate with PG content – a key marker of OA.
Synchrotron-based phase contrast imaging of cardiovascular tissue in mice—grating interferometry or phase propagation?
Trachet, Bram; Lovric, Goran; Villanueva-Perez, Pablo; et al. (2018)
Biomedical Physics & Engineering Express
Synchrotron-based visualization and segmentation of elastic lamellae in the mouse carotid artery during quasi-static pressure inflation
Trachet, Bram; Ferraro, Mauro; Lovric, Goran; et al. (2019)
Journal of the Royal Society. Interface
The impact of drought-induced root and root hair shrinkage on root-soil contact
Duddek, Patrick; Carminati, Andrea; Koebernick, Nicolai; et al. (2022)
Plant Physiology
Although root hairs significantly increased root-soil contact, in maize, their shrinkage during soil drying is initiated at relatively high soil matric potentials (between -10 and -310 kPa).
Micrometer-resolution X-ray tomographic full-volume reconstruction of an intact post-mortem juvenile rat lung
Borisova, Elena; Lovric, Goran; Miettinen, Arttu; et al. (2021)
Histochemistry and Cell Biology
In this article, we present an X-ray tomographic imaging method that is well suited for pulmonary disease studies in animal models to resolve the full pathway from gas intake to gas exchange. Current state-of-the-art synchrotron-based tomographic phase-contrast imaging methods allow for three-dimensional microscopic imaging data to be acquired non-destructively in scan times of the order of seconds with good soft tissue contrast. However, when studying multi-scale hierarchically structured objects, such as the mammalian lung, the overall sample size typically exceeds the field of view illuminated by the X-rays in a single scan and the necessity for achieving a high spatial resolution conflicts with the need to image the whole sample. Several image stitching and calibration techniques to achieve extended high-resolution fields of view have been reported, but those approaches tend to fail when imaging non-stable samples, thus precluding tomographic measurements of large biological samples, which are prone to degradation and motion during extended scan times. In this work, we demonstrate a full-volume three-dimensional reconstruction of an intact rat lung under immediate post-mortem conditions and at an isotropic voxel size of (2.75 µm)3. We present the methodology for collecting multiple local tomographies with 360° extended field of view scans followed by locally non-rigid volumetric stitching. Applied to the lung, it allows to resolve the entire pulmonary structure from the trachea down to the parenchyma in a single dataset.
Dose optimization approach to fast X-ray microtomography of the lung alveoli
Lovric, Goran; Barré, Sébastien F.; Schittny, Johannes C.; et al. (2013)
Journal of Applied Crystallography
A basic prerequisite for in vivo X-ray imaging of the lung is the exact determination of radiation dose. Achieving resolutions of the order of micrometres may become particularly challenging owing to increased dose, which in the worst case can be lethal for the imaged animal model. A framework for linking image quality to radiation dose in order to optimize experimental parameters with respect to dose reduction is presented. The approach may find application for current and future in vivo studies to facilitate proper experiment planning and radiation risk assessment on the one hand and exploit imaging capabilities on the other.
Analysis of Heart Induced Lung Motion in High-Resolution Synchrotron Based Radiography
Ganesh, Sai Krishnan; Borisova, Elena; Schlepütz, Christian M.; et al. (2021)
American Journal of Respiratory and Critical Care Medicine
Observations of root hair patterning in soils: Insights from synchrotron-based X-ray computed microtomography
Duddek, Patrick; Papritz, Andreas; Ahmed, Mutez Ali; et al. (2024)
Plant and Soil
Background and Aims: Root hair emergence is affected by heterogeneities in water availability in the growth medium. Root hairs preferentially emerge into air, whereas their emergence into water is inhibited. Yet, these results were based either on destructive methods or on roots grown on an agar-air interface. Additionally, there is a lack of knowledge about the spatial distribution of root hairs as hairs elongate radially across the rhizosphere. Therefore, root hair growth in soils remains largely unexplored. Methods: Maize (Zea Mays L.) plants were grown in microcosms which were scanned with a synchrotron-based X-ray μ CT. The distribution of root hairs along the root epidermis and radially across the rhizosphere (i.e. as function of distance from the root epidermis) was analysed using spatial point pattern analysis. Results: While hairs emerged randomly in air-filled pores, their emergence was inhibited where the root was in contact with the soil matrix. As hairs elongated radially into the soil, they were preferentially located in the close proximity of soil particles. In maize, we rarely observed root hairs penetrating into soil aggregates. Conclusion: We conclude that in maize, root hairs grow in air-filled pores at the root-soil interface, where the flow of nutrients and water is impeded. Across the rhizosphere, hairs establish contact to the soil by growing in the proximity to soil particles. The effect of hairs on uptake processes, plant anchorage and rhizosheath formation might be limited (in maize) as they hardly penetrate into soil aggregates.
Diffractive small angle X-ray scattering imaging for anisotropic structures
Kagias, Matias; Wang, Zhentian; Birkbak, Mie Elholm; et al. (2019)
Nature Communications
Insights into the micro- and nano-architecture of materials is crucial for understanding and predicting their macroscopic behaviour. In particular, for emerging applications such as meta-materials, the micrometer scale becomes highly relevant. The micro-architecture of such materials can be tailored to exhibit specific mechanical, optical or electromagnetic behaviours. Consequently, quality control at micrometer scale must be guaranteed over extended areas. Mesoscale investigations over millimetre sized areas can be performed by scanning small angle X-ray scattering methods (SAXS). However, due to their long measurement times, real time or operando investigations are hindered. Here we present a method based on X-ray diffractive optics that enables the acquisition of SAXS signals in a single shot (few milliseconds) over extended areas. This method is applicable to a wide range of X-ray sources with varying levels of spatial coherence and monochromaticity, as demonstrated from the experimental results. This enables a scalable solution of spatially resolved SAXS.

Publications 1 - 10 of 15

Goran Lovric

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