Morteza Aramesh

Last Name

Aramesh

First Name

Morteza

ORCID

0000-0003-2071-1929

Organisational unit

09831 - Aramesh, Morteza / Aramesh, Morteza

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Publications 1 - 10 of 22

Force-Controlled Formation of Dynamic Nanopores for Single-Biomolecule Sensing and Single-Cell Secretomics
Schlotter, Tilman; Weaver, Sean; Forró, Csaba; et al. (2020)
ACS Nano
Nanopore sensing of single nucleotides has emerged as a promising single-molecule technology for DNA sequencing and proteomics. Despite the conceptual simplicity of nanopores, adoption of this technology for practical applications has been limited by a lack of pore size adjustability and an inability to perform long-term recordings in complex solutions. Here we introduce a method for fast and precise on-demand formation of a nanopore with controllable size between 2 and 20 nm through force-controlled adjustment of the nanospace formed between the opening of a microfluidic device (made of silicon nitride) and a soft polymeric substrate. The introduced nanopore system enables stable measurements at arbitrary locations. By accurately positioning the nanopore in the proximity of single neurons and continuously recording single-molecule translations over several hours, we have demonstrated this is a powerful approach for single-cell proteomics and secretomics.
Localized detection of ions and biomolecules with a force-controlled scanning nanopore microscope
Aramesh, Morteza; Forró, Csaba; Dorwling-Carter, Livie; et al. (2019)
Nature Nanotechnology
Monolayer Graphene Coupled to a Flexible Plasmonic Nanograting for Ultrasensitive Strain Monitoring
Tiefenauer, Raphael F.; Dalgaty, Thomas; Keplinger, Tobias; et al. (2018)
Small
Mechanical characterization and cytocompatibility of linoleic acid modified bone cement for percutaneous cement discoplasty
Ghandour, Salim; Hong, Linglu; Aramesh, Morteza; et al. (2024)
Journal of the Mechanical Behavior of Biomedical Materials
Minimally invasive spine treatments have been sought after for elderly patients with comorbidities suffering from advanced degenerative disc disease. Percutaneous cement discoplasty (PCD) is one such technique where cement is injected into a degenerated disc with a vacuum phenomenon to relieve patients from pain. Adjacent vertebral fractures (AVFs) are however an inherent risk, particularly for osteoporotic patients, due to the high stiffness of the used cements. While low-modulus cements have been developed for vertebroplasty through the addition of linoleic acid, there are no such variations with a high-viscosity base cement, which is likely needed for the discoplasty application. Therefore, a low-modulus polymethyl methacrylate was developed by the addition of 12%vol. linoleic acid to a high-viscosity bone cement (hv-LA-PMMA). Initial experimental validation of the cement was performed by mechanical testing under compression over a period of 24 weeks, after storage in 37 °C phosphate buffer saline (PBS) solution. Furthermore, cement extracts were used to evaluate residual monomer release and the cytotoxicity of hv-LA-PMMA using fibroblastic cells. Relative to the base commercial cement, a significant reduction of Young’s modulus and compressive strength of 36% and 42% was observed, respectively. Compression-tension fatigue tests at 5 MPa gave an average fatigue limit of 31,078 cycles. This was higher than another low-modulus cement and comparable to the fatigue properties of the disc annulus tissue. Monomer release tests showed that hv-LA-PMMA had a significantly higher release between 24 h and 7 days compared to the original bone cement, similarly to other low-modulus cements. Also, the control cement showed cytocompatibility at all time points of extract collection for 20-fold dilution, while hv-LA-PMMA only showed the same for extract collections at day 7. However, the 20-fold dilution was needed for both the control and the hv-LA-PMMA extracts to demonstrate more than 70% fibroblast viability at day 7. In conclusion, the mechanical testing showed promise in the use of linoleic acid in combination with a high-viscosity PMMA cement to achieve properties adequate to the application. Further testing and in vivo studies are however required to fully evaluate the mechanical performance and biocompatibility of hv-LA-PMMA for possible future clinical application.
Induced Formation of Plasma Membrane Protrusions with Porous Materials as Instructive Surfaces
Aramesh, Morteza; Persson, Cecilia (2024)
Methods in Molecular Biology ~ Imaging Cell Signaling
The plasma membrane is a vital component in cellular processes, and its structure has a significant impact on cellular behavior. The physical characteristics of the extracellular environment, along with the presence of surface pores, can influence the formation of membrane protrusions. Nanoporous surfaces have demonstrated their capacity to induce membrane protrusions in both adherent and non-adherent cells. This chapter presents a methodology that utilizes a nanoporous substrate with nanotopographical constraints to effectively stimulate the formation of membrane protrusions in cells.
Effect of multi-modal environmental stress on dose-dependent cytotoxicity of nanodiamonds in Saccharomyces cerevisiae cells
Prasad, Karthika; Recek, Nina; Zhou, Renwu; et al. (2019)
Sustainable Materials and Technologies
Fast and Versatile Multiscale Patterning by Combining Template-Stripping with Nanotransfer Printing
Tiefenauer, Raphael F.; Tybrandt, Klas; Aramesh, Morteza; et al. (2018)
ACS Nano
Simultaneous scanning ion conductance and atomic force microscopy with a nanopore: Effect of the aperture edge on the ion current images
Dorwling-Carter, Livie; Aramesh, Morteza; Forró, Csaba; et al. (2018)
Journal of Applied Physics
Engineering T-cell Activation for Immunotherapy by Mechanical Forces
Aramesh, Morteza; Stoycheva, Diana; Raaz, Lion; et al. (2019)
Current Opinion in Biomedical Engineering
Adoptive immunotherapy relies on the isolation, modification, activation, and transfer of antigen-specific T-cells. With constant advances in bioengineering, each individual step in T-cell activation and expansion can be further improved. In this review, we discuss the current knowledge on factors influencing the mechanical forces applied on a T-cell during activation. We provide insights from ligand presentation and surface modification and present ideas of a mechanobiological signal transduction from extracellular receptor–ligand interaction to intranuclear gene expression changes as a determining factor of a T-cell response.
Real-time viscoelastic deformability cytometry: High-throughput mechanical phenotyping of liquid and solid biopsies
Asghari, Mohammad; Ivetich, Sarah Duclos; Aslan, Mahmut Kamil; et al. (2024)
Science Advances
In principle, the measurement of mechanical property differences between cancer cells and their benign counterparts enables the detection, diagnosis, and classification of diseases. Despite the existence of various mechanophenotyping methods, the ability to perform high-throughput single-cell deformability measurements on liquid and/or solid tissue biopsies remains an unmet challenge within clinical settings. To address this issue, we present an ultrahigh-throughput viscoelastic microfluidic platform able to measure the mechanical properties of single cells at rates of up to 100,000 cells per second (and up to 10,000 cells per second in real time). To showcase the utility of the presented platform in clinical scenarios, we perform single-cell phenotyping of both liquid and solid tumor biopsies, cytoskeletal drug analysis, and identification of malignant lymphocytes in peripheral blood samples. Our viscoelastic microfluidic methodology offers opportunities for high-throughput, label-free single-cell analysis, with diverse applications in clinical diagnostics and personalized medicine.

Publications 1 - 10 of 22

Morteza Aramesh

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