High-resolution imaging of 2D outer membrane protein F crystals by atomic force microscopy
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Author / Producer
Date
2013
Publication Type
Book Chapter
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yes
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Abstract
In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed.
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Publication status
published
Book title
Electron Cystallography of Soluble and Membrane Proteins
Journal / series
Volume
955
Pages / Article No.
461 - 474
Publisher
Humana Press
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Edition / version
Methods
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Date collected
Date created
Subject
Atomic force microscopy; Biological membranes; High resolution; Outer membrane protein F; Stylus artifacts; Topography
Organisational unit
03870 - Müller, Daniel J. / Müller, Daniel J.