Novel Insight into Proximal DNA Domain Interactions from Temperature‐Controlled Electrospray Ionization Mass Spectrometry
METADATA ONLY
Loading...
Date
2021-07-05
Publication Type
Journal Article
ETH Bibliography
yes
Citations
Altmetric
METADATA ONLY
Data
Rights / License
Abstract
Quadruplexes are non‐canonical nucleic acid structures essential for many cellular processes. Hybrid quadruplex‐duplex oligonucleotide assemblies comprised of multiple domains are challenging to study with conventional biophysical methods due to their structural complexity. Here, we introduce a novel method based on native mass spectrometry (MS) coupled with a custom‐built temperature‐controlled nanoelectrospray ionization (TCnESI) source designed to investigate interactions between proximal DNA domains. Thermal denaturation experiments were planned to observe unfolding of multi‐stranded oligonucleotide constructs derived from biologically relevant structures and to identify unfolding intermediates. Using the TCnESI MS, we observed changes in T m and thermodynamic characteristics of proximal DNA domains depending on the number of domains, their position, and order in a single experiment.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
60 (28)
Pages / Article No.
15390 - 15398
Publisher
Wiley-VCH
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Temperature-controlled mass spectrometry; Native mass spectrometry; Thermodynamics; Oligonucleotides; G-quadruplex
Organisational unit
03430 - Zenobi, Renato / Zenobi, Renato
02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry
Notes
Funding
178765 - Soft ionization mass spectrometry for studying noncovalent interactions (SNF)
Related publications and datasets
Is supplemented by: