Microfluidic cell culturing platform combining long-term, high-resolution imaging with impedance spectroscopy
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Author / Producer
Date
2015
Publication Type
Conference Paper
ETH Bibliography
yes
Citations
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Abstract
We present an integrated microfluidic system, which combines long-term cell culturing with electrical impedance spectroscopy (EIS) on a single chip. Non-adherent cells, such as yeast (S. cerevisiae), can be grown under constant media perfusion in a 2D layer under clamping pads that enable long-term time-lapse microscopy within the same focal plane. The addition of EIS provides a complementary method for continuous in-situ analysis of single cells and has been used here to monitor the growth rate of cell colonies in real time.
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Publication status
published
External links
Book title
Eurosensors 2015
Journal / series
Volume
120
Pages / Article No.
154 - 157
Publisher
Elsevier
Event
29th European Conference on Solid-State Transducers (EUROSENSORS 2015)
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Impedance spectroscopy; Microfluidics; Yeast
Organisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas