Single-Molecule AFM Study of DNA Damage by 1O2 Generated from Photoexcited C60
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Date
2020-09-17
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Journal Article
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Abstract
Light-induced oxidative damage of DNA by 1O2 generated from photoexcited C60 was observed at the single-molecule level by atomic force microscopy (AFM) imaging. Two types of DNA origami with uniform morphologies were immobilized on a mica surface and used as DNA substrates. Upon visible light irradiation (528 nm) in the presence of a C60 aqueous solution, the morphology changes of DNA origami substrates were observed by time-lapse AFM imaging at the single-molecule level by tracking a discrete DNA molecule. The origami showed nicked and flattened morphologies with relaxed features caused by the covalent cleavage of the DNA strands. The involvement of 1O2 in the on-surface DNA damage was clearly confirmed by AFM experiments in the presence of a 1O2 quencher and ESR measurements with a spin-trapping agent for 1O2. This study is the first example of single-molecule observation of oxidative damage of DNA by AFM with corresponding morphology changes in a photocontrolled and time-dependent manner by 1O2 generated catalytically from photoexcited C60. © 2020 American Chemical Society.
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published
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Journal / series
Volume
11 (18)
Pages / Article No.
7819 - 7826
Publisher
American Chemical Society
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03389 - Spencer, Nicholas (emeritus) / Spencer, Nicholas (emeritus)
08687 - Gruppe Yamakoshi
00009 - ETH-nahe Einheiten
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Funding
156097 - Synthesis and Study of Photoexcited Fullerenes (SNF)
183660 - Porphyrin-based imaging probe for the detection of oncogene promoter quadruplex DNA (SNF)
183660 - Porphyrin-based imaging probe for the detection of oncogene promoter quadruplex DNA (SNF)