Analysis of Major Histocompatibility Complex (MHC) Immunopeptidomes Using Mass Spectrometry
OPEN ACCESS
Loading...
Author / Producer
Date
2015-12
Publication Type
Review Article
ETH Bibliography
yes
OPEN ACCESS
Data
Rights / License
Abstract
The myriad of peptides presented at the cell surface by class I and class II major histocompatibility complex (MHC) molecules are referred to as the immunopeptidome and are of great importance for basic and translational science. For basic science, the immunopeptidome is a critical component for understanding the immune system; for translational science, exact knowledge of the immunopeptidome can directly fuel and guide the development of next-generation vaccines and immunotherapies against autoimmunity, infectious diseases, and cancers. In this mini-review, we summarize established isolation techniques as well as emerging mass spectrometry-based platforms (i.e. SWATH-MS) to identify and quantify MHC-associated peptides. We also highlight selected biological applications and discuss important current technical limitations that need to be solved to accelerate the development of this field.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
14 (12)
Pages / Article No.
3105 - 3117
Publisher
American Society for Biochemistry and Molecular Biology
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Organisational unit
03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
Notes
Funding
670821 - Proteomics 4D: The proteome in context (EC)