Direct radiocarbon analysis of exhaled air
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Date
2011-02-01
Publication Type
Conference Paper
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yes
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Abstract
Pharmacokinetic properties of organic substances in humans can be followed by isotopic labeling. For sub-milligram amounts, 14C becomes the preferable isotope and Accelerator Mass Spectrometry (AMS) its most powerful detection method. However, a widespread use of this method is partly impeded by laborious and time consuming sample preparation. We developed a method, which omits any preparation prior to AMS analysis, but allows direct measurement of CO2. The method uses a zeolite molecular sieve for CO2 trapping and requires a small gas interface, which is connected to a gas ion source of the AMS instrument. Based on the method, we developed a kit for CO2 analysis in exhaled air. It can be used to monitor catabolism of labeled compounds to CO2, a seldom studied route of elimination in pharmacokinetic studies. In addition, it provides an easy sampling method for breath tests as the erythromycin breath test, where the analysis with AMS would reduce the required 14C activity by ∼3 orders of magnitude. To demonstrate the potential of the kit in combination with the high sensitivity of AMS, we followed ethanol oxidation in human with a comestible: a brandy of 1964 vintage, enriched in 14C by ∼65% due to atmospheric nuclear bomb testing, could be used as labeled substance with an administered activity of ∼10 Bq14C only.
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published
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Journal / series
Volume
26 (2)
Pages / Article No.
287 - 292
Publisher
Royal Society of Chemistry
Event
JAAS 25th Anniversary Symposium
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08619 - Labor für Ionenstrahlphysik (LIP) / Laboratory of Ion Beam Physics (LIP)