The Bistable Behaviour of Pseudomonas putida KT2440 during PHA Depolymerization under Carbon Limitation
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Date
2017-06
Publication Type
Journal Article
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yes
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Abstract
Poly(hydroxyalkanoates) (PHAs) are bacterial polyesters offering a biodegradable alternative to petrochemical plastics. The intracellular formation and degradation of PHAs is a dynamic process that strongly depends on the availability of carbon and other nutrients. Carbon excess and nitrogen limitation are considered to favor PHA accumulation, whereas carbon limitation triggers PHA depolymerization when all other essential nutrients are present in excess. We studied the population dynamics of Pseudomonas putida KT2440 at the single cell level during different physiological conditions, favoring first PHA polymerization during growth on octanoic acid, and then PHA depolymerization during carbon limitation. PHAs accumulate intracellularly in granules, and were proposed to separate preferentially together with nucleic acids, leading to two daughter cells containing approximately equal amounts of PHA. However, we could show that such P. putida KT2440 cells show bistable behavior when exposed to carbon limitation, and separate into two subpopulations: one with high and one with low PHA. This suggests an asymmetric PHA distribution during cell division under carbon limitation, which has a significant influence on our understanding of PHA mobilization.
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published
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Book title
Journal / series
Volume
4 (2)
Pages / Article No.
58
Publisher
MDPI
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Date collected
Date created
Subject
Pseudomonas putida; Flow cytometry; Polyhydroxyalkanoate; PHA mobilization; PHA depolymerization; Starvation; Carbon limitation; BODIPY 493/503
Organisational unit
03602 - Panke, Sven / Panke, Sven