Real-time multi-analyte online monitoring of 3d cell cultures by integrated enzyme-based biosensors in hanging drop networks
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Date
2015
Publication Type
Conference Paper
ETH Bibliography
yes
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Abstract
We present the integration of enzyme-based lactate and glucose biosensors into hanging-drop networks. Hangingdrop networks are an ideal microfluidic platform for formation, cultivation, and continuous and long-term observation of 3D microtissues. The implementation of biosensors enables in-situ online monitoring of the effects of different culturing conditions and compound dosages on the microtissues. A hybrid approach including glass sensor modules embedded into a microfluidic polydimethylsiloxane (PDMS)-based chip facilitates system integration. The biosensors enable real-time recording of lactate production and glucose consumption of human colon carcinoma spheroids.
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Publication status
published
Editor
Book title
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)
Journal / series
Volume
Pages / Article No.
1625 - 1628
Publisher
IEEE
Event
18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS 2015)
Edition / version
Methods
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Date collected
Date created
Subject
Microfluidics; Hanging-drop network; Lactate; Glucose; Biosensor; 3D microtissues
Organisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas
Notes
Funding
296257 - The Body-on-a-Chip (BoC) (EC)
142440 - The Body on a Chip - Human 3-D tissue analogues in microfluidic systems (SNF)
142440 - The Body on a Chip - Human 3-D tissue analogues in microfluidic systems (SNF)