Resolution of Recombination Intermediates: Mechanisms and Regulation
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Date
2015
Publication Type
Conference Paper
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no
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Abstract
DNA strand break repair by homologous recombination leads to the formation of intermediates in which sister chromatids are covalently linked. The efficient processing of these joint molecules, which often contain four-way structures known as Holliday junctions, is necessary for efficient chromosome segregation during mitotic division. Because persistent chromosome bridges pose a threat to genome stability, cells ensure the complete elimination of joint molecules through three independent pathways. These involve (1) BLM-Topoisomerase IIIa-RMI1-RMI2 (BTR complex), (2) SLX1-SLX4-MUS81-EME1 (SLX-MUS complex), and (3) GEN1. The BTR pathway promotes the dissolution of double Holliday junctions, which avoids the formation of crossover products, prevents sister chromatid exchanges, and limits the potential for loss of heterozygosity. In contrast to BTR, the other two pathways resolve Holliday junctions by nucleolytic cleavage to yield crossover and non-crossover products. To avoid competition with BTR, the resolution pathways are restrained until the late stages of the cell cycle. The temporal regulation of the dissolution/resolution pathways is therefore critical for crossover avoidance while also ensuring that all covalent links between chromosomes are resolved before chromosome segregation.
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Publication status
published
Book title
21st Century Genetics: Genes at Work
Journal / series
Volume
80
Pages / Article No.
103 - 109
Publisher
Cold Spring Harbor Laboratory Press
Event
80th Cold Spring Harbor Symposium on Quantitative Biology
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09457 - Matos, Joao (ehemalig) / Matos, Joao (former)