Genome-wide RNAi Screening Identifies Protein Modules Required for 40S Subunit Synthesis in Human Cells
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Date
2015-12
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
Ribosome biogenesis is a highly complex process requiring many assisting factors. Studies in yeast have yielded comprehensive knowledge of the cellular machinery involved in this process. However, many aspects of ribosome synthesis are different in higher eukaryotes, and the global set of mammalian ribosome biogenesis factors remains unexplored. We used an imaging-based, genome-wide RNAi screen to find human proteins involved in 40S ribosomal subunit biogenesis. Our analysis identified ∼300 factors, many part of essential protein modules such as the small subunit (SSU) processome, the eIF3 and chaperonin complexes, and the ubiquitin-proteasome system. We demonstrate a role for the vertebrate-specific factor RBIS in ribosome synthesis, uncover a requirement for the CRL4 E3 ubiquitin ligase in nucleolar ribosome biogenesis, and reveal that intracellular glutamine synthesis supports 40S subunit production.
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Publication status
published
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Book title
Journal / series
Volume
13 (12)
Pages / Article No.
2879 - 2891
Publisher
Elsevier
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
ribosome synthesis; RNAi screening; cullin E3 ubiquitin ligase; glutamine metabolism
Organisational unit
03713 - Sauer, Uwe / Sauer, Uwe
03543 - Kutay, Ulrike / Kutay, Ulrike
02891 - ScopeM / ScopeM
Notes
Funding
144221 - Ribosome synthesis in mammalian cells (SNF)
Related publications and datasets
Is cited by: https://doi.org/10.3929/ethz-b-000682818