Phase Separation of Intrinsically Disordered Protein Polymers Mechanically Stiffens Fibrin Clots
OPEN ACCESS
Loading...
Author / Producer
Date
2020-12-15
Publication Type
Journal Article
ETH Bibliography
yes
Citations
Altmetric
OPEN ACCESS
Data
Abstract
Fibrin (Fb) networks self-assemble through the coagulation cascade and serve as the structural foundation of blood clots. Following severe trauma or drug therapy, reduced integrity of Fb networks can lead to formation of clots with inadequate mechanical properties. A key feature of therapeutic interventions for hemostasis is therefore the ability to restore mechanical strength to clots formed under coagulopathic conditions. Here, an intrinsically disordered protein based on an elastin-like polypeptide (ELP) sequence is described, which specifically binds Fb and modulates its mechanical properties. Hemostatic ELPs (hELPs) are designed containing N- and C-terminal peptide tags that are selectivity recognized by human transglutaminase factor XIIIa and covalently linked into fibrin networks via the natural coagulation cascade. Phase separation of hELPs above their lower critical solution temperature leads to stiffening and rescue of clot biophysical properties under simulated conditions of dilutive coagulopathy. In addition to phase-dependent stiffening, the resulting hELP-Fb networks exhibit resistance to plasmin degradation, reduced pore sizes, and accelerated gelation rate following initiation of clotting. These results demonstrate the ability of protein-based phase separation to modulate the physical and biochemical properties of blood clots and suggest protein phase separation as a new mechanism for achieving hemostasis in clinical settings.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
30 (51)
Pages / Article No.
2005245
Publisher
Wiley-VCH
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
clotting; coagulation; hydrogel mechanics; protein engineering; rheology
Organisational unit
09586 - Nash, Michael / Nash, Michael