Fast In Vitro Procedure to Identify Extraembryonic Differentiation Defect of Mouse Embryonic Stem Cells
OPEN ACCESS
Author / Producer
Date
2020-12-18
Publication Type
Journal Article
ETH Bibliography
yes
Citations
Altmetric
OPEN ACCESS
Data
Abstract
Mouse embryonic stem cells (mESCs) are a powerful model to study early mouse development. These blastocyst-derived cells can maintain pluripotency and differentiate into the three embryonic germ layers and an extraembryonic layer, the extraembryonic endoderm (ExEn), which shares similar molecular markers to the definitive endoderm. Here, we present a fast procedure to identify a differentiation defect of mESCs toward ExEn in vitro through the molecular and cellular characterization of embryoid bodies, followed by direct differentiation of mESCs into ExEn.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
1 (3)
Pages / Article No.
100127
Publisher
Cell Press
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Organisational unit
03983 - Ciaudo, C. (ehemalig) / Ciaudo, C. (former)
Notes
Funding
173120 - Canonical and non-canonical functions of RNA interference proteins during mouse early development (SNF)
Related publications and datasets
References: 10.1016/j.stemcr.2017.12.023