Genetic Impacts on the Structure and Mechanics of Cellulose Made by Bacteria
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Date
2025-09-04
Publication Type
Journal Article
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yes
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Abstract
The synthesis of cellulose pellicles by bacteria offers an enticing strategy for the biofabrication of sustainable materials and biomedical devices. To leverage this potential, bacterial strains that overproduce cellulose are identified through directed evolution technology. While cellulose overproduction is linked with a specific genetic mutation, the effect of such mutation on the intracellular protein landscape and on the structure and mechanical properties of the cellulose pellicles is not yet understood. Here, the proteome of bacteria evolved to overproduce cellulose is studied and its effect on the structure and mechanics of the resulting cellulose pellicles is investigated. Proteomic analysis reveals that the protein landscape of the evolved bacteria shows pronounced differences from that of native microorganisms. Thanks to concerted changes in the proteome, the evolved bacteria can generate cellulose pellicles with exquisite structure and improved mechanical properties for applications in textiles, packaging, and medical implants.
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published
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Journal / series
Volume
12 (33)
Pages / Article No.
Publisher
Wiley-VCH
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Subject
bacterial cellulose; directed evolution; fiber networks; mutations; proteomics
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Notes
Funding
204614 - Animate Materials enabled by Directed Evolution of Microorganisms (SNF)