A set of common movements within GPCR-G-protein complexes from variability analysis of cryo-EM datasets
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Date
2021-06
Publication Type
Journal Article
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Abstract
G-protein coupled receptors (GPCRs) are among the most versatile signal transducers in the cell. Once activated, GPCRs sample a large conformational space and couple to G-proteins to initiate distinct signaling pathways. The dynamical behavior of GPCR-G-protein complexes is difficult characterize structurally, and it might hinder obtaining routine high-resolution density maps in single-particle reconstructions. Here, we used variability analysis on the rhodopsin-Gi-Fab16 complex cryo-EM dataset, and the results provide insights into the dynamic nature of the receptor-complex interaction. We compare the outcome of this analysis with recent results obtained on the cannabinoid-Gi- and secretin-Gs-receptor complexes. Despite differences related to the biochemical compositions of the three samples, a set of consensus movements emerges. We anticipate that systematic variability analysis on GPCR-G-protein complexes may provide useful information not only at the biological level, but also for improving the preparation of more stable samples for cryo-EM single-particle analysis.
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published
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Journal / series
Volume
213 (2)
Pages / Article No.
107699
Publisher
Elsevier
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Edition / version
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Subject
GPCR; G-protein; Cryo-EM; Variability analysis; Inherent flexibility; Membrane proteins
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Funding
190826 - Electron-beam-enabled redox 3D printing at the nanoscale – a technology for the direct, bottom-up synthesis of high-quality inorganic materials in an in-situ SEM (SNF)