Physiological stretching induces a differential extracellular matrix gene expression response in acetabular labrum cells
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Date
2022-07
Publication Type
Journal Article
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yes
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Abstract
The acetabular labrum is a fibrocartilaginous ring surrounding the acetabulum and is important for hip stability and contact pressure dissipation through a sealing function. Injury of the labrum may contribute to hip-joint degeneration and development of secondary osteoarthritis. Understanding how extracellular matrix (ECM) production and remodelling is regulated is of key importance for successful tissue restoration. The present study hypothesised that physiological stretching enhanced the metabolic activity and altered the ECM gene expression in labrum cells. Primary bovine labrum cells were physiologically stretched for up to 5 d. 24 h after the last stretch cycle, changes in metabolic activity were measured using the PrestoBlueTM HS Cell Viability Reagent and ECM gene expression was examined using the quantitative polymerase chain reaction method. Targets of interest were further investigated using immunofluorescence and enzymelinked immunosorbent assay. Metabolic activity was not affected by the stretching (0.9746 +/- 0.0614, p > 0.05). Physiological stretching upregulated decorin (DCN) (1.8548 +/- 0.4883, p = 0.002) as well as proteoglycan 4 (PRG4) (1.7714 +/- 0.6600, p = 0.029) and downregulated biglycan (BGN) (0.7018 + 0.1567, p = 0.008), cartilage oligomeric matrix protein (COMP) (0.5747 +/- 0.2650, p = 0.029), fibronectin (FN1) (0.5832 +/- 0.0996, p < 0.001) and spondin 1 (SPON1) (0.6282 +/- 0.3624, p = 0.044) gene expression. No difference in PRG4 and DCN abundance or release could be measured. The here identified mechanosensitive targets are known to play relevant roles in tissue organisation. Therefore, physiological stretching might play a role in labrum tissue homeostasis and regeneration.
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published
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Journal / series
Volume
44
Pages / Article No.
90 - 100
Publisher
AO Research Institute Davos
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Subject
Labrum; hip; tissue remodelling; regeneration; extracellular matrix organisation; mechanical; loading; stretching; cyclic tensile strain; mechanobiology; gene expression
Organisational unit
03915 - Ferguson, Stephen / Ferguson, Stephen
Notes
Funding
176023 - Electrospun Nanofibrous Scaffolds for Acetabular Labrum Restoration (SNF)