Microfluidic platform for continuous perfusion of transwell-based barrier models
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Author / Producer
Date
2022-08
Publication Type
Conference Paper
ETH Bibliography
yes
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Abstract
Here, we present a microfluidic platform for establishing continuous perfusion in transwell-based in-vitro barrier models. We developed a poly(methylmethacrylate) (PMMA)-based microfluidic device, which allows for dynamic control of culture microenvironments in both, apical and basolateral compartments of cell-covered transwell inserts. We demonstrate the use of the device for high-resolution confocal imaging of the barrier models.
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Publication status
published
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Editor
Book title
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
Journal / series
Volume
Pages / Article No.
1703 - 1704
Publisher
Curran
Event
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Barrier model; Cell culture; Microfluidics; Organ-on-a-chip; Transwell inserts
Organisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas
Notes
Funding
180541 - “Phage therapy to treat E. coli infections using in-vitro models” (SNF)