Liquid Chromatographic Isolation of Individual Amino Acids Extracted From Sediments for Radiocarbon Analysis
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Date
2020-03
Publication Type
Journal Article
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yes
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Abstract
The “building blocks of life” are found nearly ubiquitously in the environment in the form of proteins, peptides, and single amino acids. To shed light on amino acid sources, cycling, and preservation in sedimentary environments, we present a method using high-pressure liquid chromatography to separate and isolate underivatized amino acids extracted from sediments to conduct compound-specific radiocarbon analysis. This method consists of three main steps including (1) amino acid extraction by hydrofluoric and hydrochloric acids followed by desalting, (2) liquid chromatographic isolation and purification using two complementary column chemistries, and (3) post-purification and measurement by accelerator mass spectrometry. The resulting blank of this procedure is estimated to contain 2.2 ± 1.3 μgC with 0.25 ± 0.09 Fm.
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published
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Journal / series
Volume
7
Pages / Article No.
174
Publisher
Frontiers Media
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Edition / version
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Software
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Date collected
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Subject
14C; compound-specific radiocarbon analysis; demineralization; hydrolysis; desalting; protein; peptide; purification
Organisational unit
08619 - Labor für Ionenstrahlphysik (LIP) / Laboratory of Ion Beam Physics (LIP)
03868 - Eglinton, Timothy I. (emeritus) / Eglinton, Timothy I. (emeritus)