Microfluidic generation of amino-functionalized hydrogel microbeads capable of on-bead bioassay
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Date
2019-08
Publication Type
Journal Article
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yes
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Abstract
Microfluidic generation of hydrogel microbeads is a highly efficient and reproducible approach to create various functional hydrogel beads. Here, we report a method to prepare crosslinked amino-functionalized polyethylene glycol (PEG) microbeads using a microfluidic channel. The microbeads generated from a microfluidic device were evaluated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and confocal laser scanning microscopy, respectively. We found that the microbeads were monodisperse and the amino groups were localized on the shell region of the microbeads. A swelling test exhibited compatibility with various solvents. A cell binding assay was successfully performed with RGD peptide-coupled amino-functionalized hydrogel microbeads. This strategy will enable the large production of the various functional microbeads, which can be used for solid phase peptide synthesis and on-bead bioassays.
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published
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Journal / series
Volume
10 (8)
Pages / Article No.
527
Publisher
MDPI
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Edition / version
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Date collected
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Subject
polyethylene glycol; hydrogel microbead; microfluidic channel; on-bead bioassay; peptide synthesis
Organisational unit
02891 - ScopeM / ScopeM